The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco
Late embryogenesis abundant (LEA) proteins accumulate in the late stage of plant seed development, and are upregulated in most plants during drought, cold, heat, or salinity stress. LEA proteins can be classified by amino-acid sequence into seven groups. Dehydrins belong to LEA protein group II. In...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2019-06-01
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| Series: | Crop Journal |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514119300054 |
| _version_ | 1818430922677026816 |
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| author | Yang Liu Daxing Li Qiping Song Tianpeng Zhang Dequan Li Xinghong Yang |
| author_facet | Yang Liu Daxing Li Qiping Song Tianpeng Zhang Dequan Li Xinghong Yang |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Late embryogenesis abundant (LEA) proteins accumulate in the late stage of plant seed development, and are upregulated in most plants during drought, cold, heat, or salinity stress. LEA proteins can be classified by amino-acid sequence into seven groups. Dehydrins belong to LEA protein group II. In previous studies, the maize KS type dehydrin ZmDHN13 increased the tolerance of transgenic tobacco to oxidative stress. In the present study, ZmDHN13 was identified under copper stress conditions, and the protein was then characterized using transgenic yeast and tobacco plants to investigate its functions. ZmDHN13 bound Cu2+. Its overexpression in transgenic tobacco conferred tolerance to copper stress by binding metals and reducing the accumulation of reactive oxygen species (ROS). Three conserved domains displayed a cooperative effect under copper stress conditions. Keywords: Maize, LEA proteins, Dehydrins, ZmDHN13, Copper stress |
| first_indexed | 2024-12-14T15:41:07Z |
| format | Article |
| id | doaj.art-32a77b0359ff4e548c77a7933e622205 |
| institution | Directory Open Access Journal |
| issn | 2214-5141 |
| language | English |
| last_indexed | 2024-12-14T15:41:07Z |
| publishDate | 2019-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj.art-32a77b0359ff4e548c77a7933e6222052022-12-21T22:55:37ZengKeAi Communications Co., Ltd.Crop Journal2214-51412019-06-0173403410The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobaccoYang Liu0Daxing Li1Qiping Song2Tianpeng Zhang3Dequan Li4Xinghong Yang5Corresponding authors.; State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, ChinaState Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, ChinaCorresponding authors.; State Key Laboratory of Crop Biology/Shandong Key Laboratory of Crop Biology/College of Life Sciences, Shandong Agricultural University, Tai'an 271018, Shandong, ChinaLate embryogenesis abundant (LEA) proteins accumulate in the late stage of plant seed development, and are upregulated in most plants during drought, cold, heat, or salinity stress. LEA proteins can be classified by amino-acid sequence into seven groups. Dehydrins belong to LEA protein group II. In previous studies, the maize KS type dehydrin ZmDHN13 increased the tolerance of transgenic tobacco to oxidative stress. In the present study, ZmDHN13 was identified under copper stress conditions, and the protein was then characterized using transgenic yeast and tobacco plants to investigate its functions. ZmDHN13 bound Cu2+. Its overexpression in transgenic tobacco conferred tolerance to copper stress by binding metals and reducing the accumulation of reactive oxygen species (ROS). Three conserved domains displayed a cooperative effect under copper stress conditions. Keywords: Maize, LEA proteins, Dehydrins, ZmDHN13, Copper stresshttp://www.sciencedirect.com/science/article/pii/S2214514119300054 |
| spellingShingle | Yang Liu Daxing Li Qiping Song Tianpeng Zhang Dequan Li Xinghong Yang The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco Crop Journal |
| title | The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco |
| title_full | The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco |
| title_fullStr | The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco |
| title_full_unstemmed | The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco |
| title_short | The maize late embryogenesis abundant protein ZmDHN13 positively regulates copper tolerance in transgenic yeast and tobacco |
| title_sort | maize late embryogenesis abundant protein zmdhn13 positively regulates copper tolerance in transgenic yeast and tobacco |
| url | http://www.sciencedirect.com/science/article/pii/S2214514119300054 |
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