The impact of stress on the transcriptomic signature of iNKT1 cells
Invariant natural killer T (iNKT) cells develop in thymus before emigrating and settling peripheral tissues and organs. In contrast to regular naïve T cells, most iNKT cells do not continuously recirculate but are rather sessile and can adopt phenotypically as well as functionally to their tissue en...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580821002570 |
| _version_ | 1819004082689409024 |
|---|---|
| author | Georgia Papadogianni Inga Ravens Ahmed Hassan Oliver Dittrich-Breiholz Günter Bernhardt Hristo Georgiev |
| author_facet | Georgia Papadogianni Inga Ravens Ahmed Hassan Oliver Dittrich-Breiholz Günter Bernhardt Hristo Georgiev |
| author_sort | Georgia Papadogianni |
| collection | DOAJ |
| description | Invariant natural killer T (iNKT) cells develop in thymus before emigrating and settling peripheral tissues and organs. In contrast to regular naïve T cells, most iNKT cells do not continuously recirculate but are rather sessile and can adopt phenotypically as well as functionally to their tissue environment. To explore this in more detail, we focused on the most widely distributed CD4+iNKT1 cells and compared the transcriptome of cells isolated from liver and spleen. Whereas there are only very few genuine differences in the transcriptomes of CD4+iNKT1 cells of these two organs, the mode of cell isolation left clear marks in the transcriptomic signature. In contrast to liver cell isolated in the cold, cells prepared by enzymatic tissue digestion upregulated quickly a series of genes known to respond to stress. Therefore, to avoid erroneous conclusions, a comparison of expression profiles must take into consideration the history of cell preparation. |
| first_indexed | 2024-12-20T23:31:15Z |
| format | Article |
| id | doaj.art-32a7c5dcb434415db41314df195beb28 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-20T23:31:15Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-32a7c5dcb434415db41314df195beb282022-12-21T19:23:18ZengElsevierBiochemistry and Biophysics Reports2405-58082021-12-0128101163The impact of stress on the transcriptomic signature of iNKT1 cellsGeorgia Papadogianni0Inga Ravens1Ahmed Hassan2Oliver Dittrich-Breiholz3Günter Bernhardt4Hristo Georgiev5Institute of Immunology, Hannover Medical School, 30625, Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625, Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625, Hannover, GermanyResearch Core Unit Genomics, Hannover Medical School, 30625, Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625, Hannover, GermanyInstitute of Immunology, Hannover Medical School, 30625, Hannover, Germany; Corresponding author.Invariant natural killer T (iNKT) cells develop in thymus before emigrating and settling peripheral tissues and organs. In contrast to regular naïve T cells, most iNKT cells do not continuously recirculate but are rather sessile and can adopt phenotypically as well as functionally to their tissue environment. To explore this in more detail, we focused on the most widely distributed CD4+iNKT1 cells and compared the transcriptome of cells isolated from liver and spleen. Whereas there are only very few genuine differences in the transcriptomes of CD4+iNKT1 cells of these two organs, the mode of cell isolation left clear marks in the transcriptomic signature. In contrast to liver cell isolated in the cold, cells prepared by enzymatic tissue digestion upregulated quickly a series of genes known to respond to stress. Therefore, to avoid erroneous conclusions, a comparison of expression profiles must take into consideration the history of cell preparation.http://www.sciencedirect.com/science/article/pii/S2405580821002570iNKT cellsStress responseCell isolationTranscriptomeP2X7 |
| spellingShingle | Georgia Papadogianni Inga Ravens Ahmed Hassan Oliver Dittrich-Breiholz Günter Bernhardt Hristo Georgiev The impact of stress on the transcriptomic signature of iNKT1 cells Biochemistry and Biophysics Reports iNKT cells Stress response Cell isolation Transcriptome P2X7 |
| title | The impact of stress on the transcriptomic signature of iNKT1 cells |
| title_full | The impact of stress on the transcriptomic signature of iNKT1 cells |
| title_fullStr | The impact of stress on the transcriptomic signature of iNKT1 cells |
| title_full_unstemmed | The impact of stress on the transcriptomic signature of iNKT1 cells |
| title_short | The impact of stress on the transcriptomic signature of iNKT1 cells |
| title_sort | impact of stress on the transcriptomic signature of inkt1 cells |
| topic | iNKT cells Stress response Cell isolation Transcriptome P2X7 |
| url | http://www.sciencedirect.com/science/article/pii/S2405580821002570 |
| work_keys_str_mv | AT georgiapapadogianni theimpactofstressonthetranscriptomicsignatureofinkt1cells AT ingaravens theimpactofstressonthetranscriptomicsignatureofinkt1cells AT ahmedhassan theimpactofstressonthetranscriptomicsignatureofinkt1cells AT oliverdittrichbreiholz theimpactofstressonthetranscriptomicsignatureofinkt1cells AT gunterbernhardt theimpactofstressonthetranscriptomicsignatureofinkt1cells AT hristogeorgiev theimpactofstressonthetranscriptomicsignatureofinkt1cells AT georgiapapadogianni impactofstressonthetranscriptomicsignatureofinkt1cells AT ingaravens impactofstressonthetranscriptomicsignatureofinkt1cells AT ahmedhassan impactofstressonthetranscriptomicsignatureofinkt1cells AT oliverdittrichbreiholz impactofstressonthetranscriptomicsignatureofinkt1cells AT gunterbernhardt impactofstressonthetranscriptomicsignatureofinkt1cells AT hristogeorgiev impactofstressonthetranscriptomicsignatureofinkt1cells |