miRNA polymorphisms and risk of premature coronary artery disease
Objective: Several microRNA (miRNA) polymorphisms have been associated with susceptibility to specific health disorders, including cardiovascular diseases. The aim of the present study was to investigate whether four well-studied miRNA polymorphisms in non-Caucasian populations, namely miR146a G>...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-07-01
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| Series: | Hellenic Journal of Cardiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1109966620300312 |
| _version_ | 1818885049649463296 |
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| author | Konstantinos Agiannitopoulos Pinelopi Samara Miranta Papadopoulou Astradeni Efthymiadou Eirini Papadopoulou Georgios N. Tsaousis George Mertzanos Dimitrios Babalis Klea Lamnissou |
| author_facet | Konstantinos Agiannitopoulos Pinelopi Samara Miranta Papadopoulou Astradeni Efthymiadou Eirini Papadopoulou Georgios N. Tsaousis George Mertzanos Dimitrios Babalis Klea Lamnissou |
| author_sort | Konstantinos Agiannitopoulos |
| collection | DOAJ |
| description | Objective: Several microRNA (miRNA) polymorphisms have been associated with susceptibility to specific health disorders, including cardiovascular diseases. The aim of the present study was to investigate whether four well-studied miRNA polymorphisms in non-Caucasian populations, namely miR146a G>C (rs2910164), miR149 C>T (rs2292832), miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), contribute to the risk for the development of premature Coronary Artery Disease (CAD) in the Greek population. Methods: We used a case-control study to examine these associations in 400 individuals: 200 CAD patients [including a subgroup of myocardial infraction (MI) patients] and 200 healthy controls, all of Greek origin. MiRNA polymorphisms were genotyped using three different assays: Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP), High resolution Melting (HRM) and Sanger sequencing. Results: Two of these polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444) were found to be strongly associated with increased risk for CAD (p=0.0388 and p=0.0013, respectively) and for MI (p=0.0281 and p=0.0273, respectively). Furthermore, miR146C-miR149C-miR196T-miR499G allele combination appeared to be significantly related to CAD (p=0.0185) and MI (p=0.0337) prevalence. Conclusions: Our results suggest that at least two of the studied polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), as well as the miR146C-miR149C-miR196T-miR499G allele combination could represent useful biomarkers of CAD and/or MI susceptibility in the Greek population. These special genetic characteristics, in combination with environmental factors and personal habits, might contribute to CAD and/or MI prevalence. |
| first_indexed | 2024-12-19T15:59:16Z |
| format | Article |
| id | doaj.art-32a9235e2789465f9ea0e5630ba2752f |
| institution | Directory Open Access Journal |
| issn | 1109-9666 |
| language | English |
| last_indexed | 2024-12-19T15:59:16Z |
| publishDate | 2021-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Hellenic Journal of Cardiology |
| spelling | doaj.art-32a9235e2789465f9ea0e5630ba2752f2022-12-21T20:14:58ZengElsevierHellenic Journal of Cardiology1109-96662021-07-01624278284miRNA polymorphisms and risk of premature coronary artery diseaseKonstantinos Agiannitopoulos0Pinelopi Samara1Miranta Papadopoulou2Astradeni Efthymiadou3Eirini Papadopoulou4Georgios N. Tsaousis5George Mertzanos6Dimitrios Babalis7Klea Lamnissou8Division of Genetics & Biotechnology, Department of Biology, National & Kapodistrian University of Athens, Athens, Greece; Corresponding author. Konstantinos Agiannitopoulos, Division of Genetics & Biotechnology, Department of Biology, National & Kapodistrian University of Athens, Panepistimiopolis, Illisia, Athens, 15784, Greece. Tel: +302107274636, Fax: +302107274318.Division of Genetics & Biotechnology, Department of Biology, National & Kapodistrian University of Athens, Athens, GreeceDivision of Genetics & Biotechnology, Department of Biology, National & Kapodistrian University of Athens, Athens, GreeceDivision of Genetics & Biotechnology, Department of Biology, National & Kapodistrian University of Athens, Athens, GreeceGenekor M.S.A, Athens, GreeceGenekor M.S.A, Athens, GreeceDepartment of Cardiology, “KAT” General Hospital, Athens, GreeceDepartment of Cardiology, “KAT” General Hospital, Athens, GreeceDivision of Genetics & Biotechnology, Department of Biology, National & Kapodistrian University of Athens, Athens, GreeceObjective: Several microRNA (miRNA) polymorphisms have been associated with susceptibility to specific health disorders, including cardiovascular diseases. The aim of the present study was to investigate whether four well-studied miRNA polymorphisms in non-Caucasian populations, namely miR146a G>C (rs2910164), miR149 C>T (rs2292832), miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), contribute to the risk for the development of premature Coronary Artery Disease (CAD) in the Greek population. Methods: We used a case-control study to examine these associations in 400 individuals: 200 CAD patients [including a subgroup of myocardial infraction (MI) patients] and 200 healthy controls, all of Greek origin. MiRNA polymorphisms were genotyped using three different assays: Polymerase chain reaction - restriction fragment length polymorphism (PCR-RFLP), High resolution Melting (HRM) and Sanger sequencing. Results: Two of these polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444) were found to be strongly associated with increased risk for CAD (p=0.0388 and p=0.0013, respectively) and for MI (p=0.0281 and p=0.0273, respectively). Furthermore, miR146C-miR149C-miR196T-miR499G allele combination appeared to be significantly related to CAD (p=0.0185) and MI (p=0.0337) prevalence. Conclusions: Our results suggest that at least two of the studied polymorphisms, miR196a2 C>T (rs11614913) and miR499 A>G (rs3746444), as well as the miR146C-miR149C-miR196T-miR499G allele combination could represent useful biomarkers of CAD and/or MI susceptibility in the Greek population. These special genetic characteristics, in combination with environmental factors and personal habits, might contribute to CAD and/or MI prevalence.http://www.sciencedirect.com/science/article/pii/S1109966620300312Coronary Artery Disease (CAD)Myocardial Infarction (MI)microRNAs (miRNAs)polymorphismsrisk factor |
| spellingShingle | Konstantinos Agiannitopoulos Pinelopi Samara Miranta Papadopoulou Astradeni Efthymiadou Eirini Papadopoulou Georgios N. Tsaousis George Mertzanos Dimitrios Babalis Klea Lamnissou miRNA polymorphisms and risk of premature coronary artery disease Hellenic Journal of Cardiology Coronary Artery Disease (CAD) Myocardial Infarction (MI) microRNAs (miRNAs) polymorphisms risk factor |
| title | miRNA polymorphisms and risk of premature coronary artery disease |
| title_full | miRNA polymorphisms and risk of premature coronary artery disease |
| title_fullStr | miRNA polymorphisms and risk of premature coronary artery disease |
| title_full_unstemmed | miRNA polymorphisms and risk of premature coronary artery disease |
| title_short | miRNA polymorphisms and risk of premature coronary artery disease |
| title_sort | mirna polymorphisms and risk of premature coronary artery disease |
| topic | Coronary Artery Disease (CAD) Myocardial Infarction (MI) microRNAs (miRNAs) polymorphisms risk factor |
| url | http://www.sciencedirect.com/science/article/pii/S1109966620300312 |
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