Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate
Radiopharmaceuticals based on 90Y are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a 90Sr/90Y generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated...
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VINCA Institute of Nuclear Sciences
2012-01-01
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| Series: | Nuclear Technology and Radiation Protection |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/1451-3994/2012/1451-39941203260P.pdf |
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| author | Petrović Đorđe Ž. Nikolić Nadežda S. Stanković Dragana T. Đokić Divna Đ. |
| author_facet | Petrović Đorđe Ž. Nikolić Nadežda S. Stanković Dragana T. Đokić Divna Đ. |
| author_sort | Petrović Đorđe Ž. |
| collection | DOAJ |
| description | Radiopharmaceuticals based on 90Y are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a 90Sr/90Y generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA0,Tyr3] octreotate and the preparation of [90Y-DOTA0,Tyr3] octreotate (90Y-DOTATATE) for peptide receptore radionuclide therapy. 90Sr/90Y generator was based on the electrochemical separation of 90Y from 90Sr in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionuclidic purity of the 90Y solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mg DOTATATE) with 90YCl3 was performed at 95°C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for 90Y. The achieved efficiency of the 90Sr/90Y generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with 90Y exhibited a high efficiency, too: there was less than 1% of 90Y3+in the 90Y-DOTATATE. |
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| issn | 1451-3994 |
| language | English |
| last_indexed | 2024-04-14T00:41:22Z |
| publishDate | 2012-01-01 |
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| series | Nuclear Technology and Radiation Protection |
| spelling | doaj.art-32ac7d980d2d4621859753f820e6aa7a2022-12-22T02:22:11ZengVINCA Institute of Nuclear SciencesNuclear Technology and Radiation Protection1451-39942012-01-0127326026810.2298/NTRP1203260PElectrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotatePetrović Đorđe Ž.Nikolić Nadežda S.Stanković Dragana T.Đokić Divna Đ.Radiopharmaceuticals based on 90Y are widely used in the treatment of malignant deseases. In order to meet the requirements for their future application, a 90Sr/90Y generator was developed and 90Y eluted from this locally produced generator was used for the radiolabelling of the DOTA-conjugated somatostatin analog [DOTA0,Tyr3] octreotate and the preparation of [90Y-DOTA0,Tyr3] octreotate (90Y-DOTATATE) for peptide receptore radionuclide therapy. 90Sr/90Y generator was based on the electrochemical separation of 90Y from 90Sr in a two-cycle electrolysis procedure. Three electrode cells were used to perform both electrolyses. In both cycles, working electrodes were kept on constant potential. The pH of the solution was adjusted to 2.7 of the value before the electrolyses. The radionuclidic purity of the 90Y solution was analysed by ITLC and extraction paper chromatography. The labelling of peptide (100 mg DOTATATE) with 90YCl3 was performed at 95°C for 30 minutes. Radiochemical purity was determined by HPLC and chromatographic separation, using a solid SepPak C-18 column. Results obtained confirmed the efficiency of our electrochemical separation technique and quality control methods for 90Y. The achieved efficiency of the 90Sr/90Y generator above 96% of the theoretical value represents a good basis for the further development of this generator. The labelling of the DOTATATE with 90Y exhibited a high efficiency, too: there was less than 1% of 90Y3+in the 90Y-DOTATATE.http://www.doiserbia.nb.rs/img/doi/1451-3994/2012/1451-39941203260P.pdfradionuclide therapy90Y90Sr/90Y generatorradiolabelling90Y-DOTATATE |
| spellingShingle | Petrović Đorđe Ž. Nikolić Nadežda S. Stanković Dragana T. Đokić Divna Đ. Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate Nuclear Technology and Radiation Protection radionuclide therapy 90Y 90Sr/90Y generator radiolabelling 90Y-DOTATATE |
| title | Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate |
| title_full | Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate |
| title_fullStr | Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate |
| title_full_unstemmed | Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate |
| title_short | Electrochemical separation of 90-yttrium in the electrochemical 90Sr/90Y generator and its use for radiolabelling of DOTA-conjugated somatostatin analog [DOTA0, Tyr3] octreotate |
| title_sort | electrochemical separation of 90 yttrium in the electrochemical 90sr 90y generator and its use for radiolabelling of dota conjugated somatostatin analog dota0 tyr3 octreotate |
| topic | radionuclide therapy 90Y 90Sr/90Y generator radiolabelling 90Y-DOTATATE |
| url | http://www.doiserbia.nb.rs/img/doi/1451-3994/2012/1451-39941203260P.pdf |
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