Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2
Rabbit Haemorrhagic Disease Virus 2 (RHDV2, recently named <i>Lagovirus europaeus</i>/GI.2) was first reported in France in 2010 and has spread globally since then, replacing most of the circulating former RHDV (genotype GI.1) in many countries. The detection and differentiation of both...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/11/3/401 |
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| author | Alba Fresco-Taboada Mercedes Montón Istar Tapia Elena Soria Juan Bárcena Cécile Guillou-Cloarec Ghislaine Le Gall-Reculé Esther Blanco Paloma Rueda |
| author_facet | Alba Fresco-Taboada Mercedes Montón Istar Tapia Elena Soria Juan Bárcena Cécile Guillou-Cloarec Ghislaine Le Gall-Reculé Esther Blanco Paloma Rueda |
| author_sort | Alba Fresco-Taboada |
| collection | DOAJ |
| description | Rabbit Haemorrhagic Disease Virus 2 (RHDV2, recently named <i>Lagovirus europaeus</i>/GI.2) was first reported in France in 2010 and has spread globally since then, replacing most of the circulating former RHDV (genotype GI.1) in many countries. The detection and differentiation of both genotypes is of crucial importance for the surveillance of the disease. In this article, a duplex lateral flow assay (LFA) for antigen detection is described and evaluated, providing the first description of a quick and easy-to-use test that allows for the simultaneous detection and differentiation of RHDV genotypes GI.1 and GI.2. A panel of GI.1- or GI.2-infected and non-infected rabbit liver samples and liver exudates (136 samples) was analysed, obtaining a total sensitivity of 94.4% and specificity of 100%. These data confirm that the developed duplex LFA can be used as a reliable diagnostic test for RHD surveillance, especially in farms and the field. |
| first_indexed | 2024-03-09T13:49:53Z |
| format | Article |
| id | doaj.art-32acaf4961744c5ba393a5409da75087 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-09T13:49:53Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-32acaf4961744c5ba393a5409da750872023-11-30T20:51:40ZengMDPI AGBiology2079-77372022-03-0111340110.3390/biology11030401Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2Alba Fresco-Taboada0Mercedes Montón1Istar Tapia2Elena Soria3Juan Bárcena4Cécile Guillou-Cloarec5Ghislaine Le Gall-Reculé6Esther Blanco7Paloma Rueda8Eurofins Ingenasa S.A., 28037 Madrid, SpainEurofins Ingenasa S.A., 28037 Madrid, SpainEurofins Ingenasa S.A., 28037 Madrid, SpainEurofins Ingenasa S.A., 28037 Madrid, SpainCentro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, SpainFrench Agency for Food, Environmental and Occupational Health & Safety (Anses), Avian and Rabbit Virology, Immunology and Parasitology Unit, Ploufragan-Plouzané-Niort Laboratory, 22440 Ploufragan, FranceFrench Agency for Food, Environmental and Occupational Health & Safety (Anses), Avian and Rabbit Virology, Immunology and Parasitology Unit, Ploufragan-Plouzané-Niort Laboratory, 22440 Ploufragan, FranceCentro de Investigación en Sanidad Animal (CISA-INIA/CSIC), Valdeolmos, 28130 Madrid, SpainEurofins Ingenasa S.A., 28037 Madrid, SpainRabbit Haemorrhagic Disease Virus 2 (RHDV2, recently named <i>Lagovirus europaeus</i>/GI.2) was first reported in France in 2010 and has spread globally since then, replacing most of the circulating former RHDV (genotype GI.1) in many countries. The detection and differentiation of both genotypes is of crucial importance for the surveillance of the disease. In this article, a duplex lateral flow assay (LFA) for antigen detection is described and evaluated, providing the first description of a quick and easy-to-use test that allows for the simultaneous detection and differentiation of RHDV genotypes GI.1 and GI.2. A panel of GI.1- or GI.2-infected and non-infected rabbit liver samples and liver exudates (136 samples) was analysed, obtaining a total sensitivity of 94.4% and specificity of 100%. These data confirm that the developed duplex LFA can be used as a reliable diagnostic test for RHD surveillance, especially in farms and the field.https://www.mdpi.com/2079-7737/11/3/401Rabbit haemorrhagic disease viruslateral flow assayantigen detection |
| spellingShingle | Alba Fresco-Taboada Mercedes Montón Istar Tapia Elena Soria Juan Bárcena Cécile Guillou-Cloarec Ghislaine Le Gall-Reculé Esther Blanco Paloma Rueda Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2 Biology Rabbit haemorrhagic disease virus lateral flow assay antigen detection |
| title | Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2 |
| title_full | Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2 |
| title_fullStr | Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2 |
| title_full_unstemmed | Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2 |
| title_short | Development and Evaluation of a Duplex Lateral Flow Assay for the Detection and Differentiation between Rabbit Haemorrhagic Disease Virus <i>Lagovirus europaeus</i>/GI.1 and /GI.2 |
| title_sort | development and evaluation of a duplex lateral flow assay for the detection and differentiation between rabbit haemorrhagic disease virus i lagovirus europaeus i gi 1 and gi 2 |
| topic | Rabbit haemorrhagic disease virus lateral flow assay antigen detection |
| url | https://www.mdpi.com/2079-7737/11/3/401 |
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