Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compounds
Planarians are promising model animals and are increasing in importance for ecotoxicological studies. However, there is scarce to no information regarding polycyclic aromatic hydrocarbons (PAHs) specific toxicity towards planarians at the biochemical level. We aimed to uncover how vital physiologica...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Environmental Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666765722000370 |
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| author | Fátima C.P. Simão Andreia C.M. Rodrigues Carlos Gravato Amadeu M.V.M. Soares João L.T. Pestana |
| author_facet | Fátima C.P. Simão Andreia C.M. Rodrigues Carlos Gravato Amadeu M.V.M. Soares João L.T. Pestana |
| author_sort | Fátima C.P. Simão |
| collection | DOAJ |
| description | Planarians are promising model animals and are increasing in importance for ecotoxicological studies. However, there is scarce to no information regarding polycyclic aromatic hydrocarbons (PAHs) specific toxicity towards planarians at the biochemical level. We aimed to uncover how vital physiological functions, such as, neurophysiology, detoxification and oxidative stress status, and cellular energy allocation are affected on the freshwater planarian Girardia tigrina by low and high molecular weight PAHs – phenanthrene, pyrene and benzo[a]pyrene (B[a]P). Phenanthrene affected planarian antioxidant defences and detoxification, increasing glutathione levels and activity of glutathione-S-transferase (GST), the latter in a dose-dependent way (from 75 to 300 µg L−1). Phenanthrene also decreased carbohydrates in planarians exposed to 150 and 300 µg L−1. Pyrene caused higher oxygen consumption and decreased energy budget in planarians exposed to 75 and 150 µg L−1. B[a]P exposure decreased G. tigrina GST activity in a dose-dependent way. Lower lipid peroxidation levels were measured in planarians exposed to 2.5 and 75 µg L−1 of pyrene and at 37.5, 75 and 150 µg L−1 of B[a]P. The present data indicate that each of these PAHs seems to elicit distinct biochemical responses in planarians, possibly pointing to different biochemical interactions and/or metabolisation of each compound, with B[a]P causing the most severe effects. |
| first_indexed | 2024-04-13T21:39:39Z |
| format | Article |
| id | doaj.art-32ad8e696be54578875c5c64e38f17fc |
| institution | Directory Open Access Journal |
| issn | 2666-7657 |
| language | English |
| last_indexed | 2024-04-13T21:39:39Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environmental Advances |
| spelling | doaj.art-32ad8e696be54578875c5c64e38f17fc2022-12-22T02:28:49ZengElsevierEnvironmental Advances2666-76572022-07-018100201Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compoundsFátima C.P. Simão0Andreia C.M. Rodrigues1Carlos Gravato2Amadeu M.V.M. Soares3João L.T. Pestana4CESAM – Centro de Estudos do Ambiente e do Mar, Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalCESAM – Centro de Estudos do Ambiente e do Mar, Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal; Corresponding author.Faculdade de Ciências & CESAM, Universidade de Lisboa, 1749-016 Campo Grande, Lisboa, PortugalCESAM – Centro de Estudos do Ambiente e do Mar, Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalCESAM – Centro de Estudos do Ambiente e do Mar, Departamento de Biologia, Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, PortugalPlanarians are promising model animals and are increasing in importance for ecotoxicological studies. However, there is scarce to no information regarding polycyclic aromatic hydrocarbons (PAHs) specific toxicity towards planarians at the biochemical level. We aimed to uncover how vital physiological functions, such as, neurophysiology, detoxification and oxidative stress status, and cellular energy allocation are affected on the freshwater planarian Girardia tigrina by low and high molecular weight PAHs – phenanthrene, pyrene and benzo[a]pyrene (B[a]P). Phenanthrene affected planarian antioxidant defences and detoxification, increasing glutathione levels and activity of glutathione-S-transferase (GST), the latter in a dose-dependent way (from 75 to 300 µg L−1). Phenanthrene also decreased carbohydrates in planarians exposed to 150 and 300 µg L−1. Pyrene caused higher oxygen consumption and decreased energy budget in planarians exposed to 75 and 150 µg L−1. B[a]P exposure decreased G. tigrina GST activity in a dose-dependent way. Lower lipid peroxidation levels were measured in planarians exposed to 2.5 and 75 µg L−1 of pyrene and at 37.5, 75 and 150 µg L−1 of B[a]P. The present data indicate that each of these PAHs seems to elicit distinct biochemical responses in planarians, possibly pointing to different biochemical interactions and/or metabolisation of each compound, with B[a]P causing the most severe effects.http://www.sciencedirect.com/science/article/pii/S2666765722000370Epibenthic freshwater planariansPolycyclic aromatic hydrocarbonsOxidative stress statusEnergy budgetLipid peroxidation |
| spellingShingle | Fátima C.P. Simão Andreia C.M. Rodrigues Carlos Gravato Amadeu M.V.M. Soares João L.T. Pestana Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compounds Environmental Advances Epibenthic freshwater planarians Polycyclic aromatic hydrocarbons Oxidative stress status Energy budget Lipid peroxidation |
| title | Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compounds |
| title_full | Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compounds |
| title_fullStr | Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compounds |
| title_full_unstemmed | Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compounds |
| title_short | Oxidative status of planarians is differently affected by PAHs: 3-5 Benzene ring compounds |
| title_sort | oxidative status of planarians is differently affected by pahs 3 5 benzene ring compounds |
| topic | Epibenthic freshwater planarians Polycyclic aromatic hydrocarbons Oxidative stress status Energy budget Lipid peroxidation |
| url | http://www.sciencedirect.com/science/article/pii/S2666765722000370 |
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