Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions
One of the diseases with the greatest economic impact on coffee cultivation worldwide and particularly in Peru is coffee rust (<i>Hemileia vastatrix</i>). The search for sustainable control methods as disease management strategies in coffee cultivation is necessary. The objective of this...
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MDPI AG
2023-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/5/1166 |
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| author | Luis Morales-Aranibar Francisca Elena Yucra Yucra Nivia Marisol Pilares Estrada Policarpo Quispe Flores Renatto Nicolino Motta Zevallos Juan Carlos Llanos Zegarra Uriel Palomino Trujillo Carlos Genaro Morales Aranibar Hebert Hernán Soto Gonzales Jorge González Aguilera Leandris Argentel-Martínez Sucleidi Nápoles Vinent |
| author_facet | Luis Morales-Aranibar Francisca Elena Yucra Yucra Nivia Marisol Pilares Estrada Policarpo Quispe Flores Renatto Nicolino Motta Zevallos Juan Carlos Llanos Zegarra Uriel Palomino Trujillo Carlos Genaro Morales Aranibar Hebert Hernán Soto Gonzales Jorge González Aguilera Leandris Argentel-Martínez Sucleidi Nápoles Vinent |
| author_sort | Luis Morales-Aranibar |
| collection | DOAJ |
| description | One of the diseases with the greatest economic impact on coffee cultivation worldwide and particularly in Peru is coffee rust (<i>Hemileia vastatrix</i>). The search for sustainable control methods as disease management strategies in coffee cultivation is necessary. The objective of this research was to determine the effectiveness of five biopesticides based on lemon verbena (<i>Cymbopogon citratus</i>) for the control of rust applied in laboratory and field conditions to allow the recovery of coffee (<i>Coffea arabica</i> L. var. typica) in La Convención, Cusco, Peru. Five biopesticides (oil, macerate, infusion, hydrolate and Biol) and four concentrations (0, 15, 20 and 25%) were evaluated. The biopesticides were evaluated under laboratory conditions (light and dark) at different concentrations. The design used was completely randomized in a factorial scheme. The biopesticides were incorporated into the culture medium and inoculated with 400 uredospores of rust, and the germination percentage was evaluated. Under field conditions, the biopesticides at the same concentrations were evaluated for 4 weeks after application. Under these field conditions, the incidence, severity and area under the disease progress curve (AUDPC) of selected plants with a natural degree of infection were evaluated. In the laboratory, the results showed that all biopesticides were effective in reducing the germination of rust uredospores to values <1% of germination in relation to the control, which showed values of 61% and 75% in the light and dark, respectively, independent of the concentration used, with no significant differences between them. In the field, 25% oil promoted the best response with values <1% and 0% incidence and severity, respectively, in the first two weeks after application. The AUDPC for this same treatment showed values of 7 in relation to 1595 of the control. <i>Cymbopogon citratus</i> oil is an excellent biopesticide that can be used to control coffee rust. |
| first_indexed | 2024-03-11T07:13:23Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T07:13:23Z |
| publishDate | 2023-03-01 |
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| spelling | doaj.art-32ae6ec103e14835b28de83d83c3d77b2023-11-17T08:25:03ZengMDPI AGPlants2223-77472023-03-01125116610.3390/plants12051166Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field ConditionsLuis Morales-Aranibar0Francisca Elena Yucra Yucra1Nivia Marisol Pilares Estrada2Policarpo Quispe Flores3Renatto Nicolino Motta Zevallos4Juan Carlos Llanos Zegarra5Uriel Palomino Trujillo6Carlos Genaro Morales Aranibar7Hebert Hernán Soto Gonzales8Jorge González Aguilera9Leandris Argentel-Martínez10Sucleidi Nápoles Vinent11National Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, PeruNational Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, PeruNational Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, PeruNational Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, PeruNational Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, PeruNational Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, PeruNational Intercultural University of Quillabamba, La Convenciòn, Cusco 08741, PeruJorge Basadre Grohmann National University (UNJBG), Tacna 23001, PeruNational University of Moquegua (UNAM), Ilo 18601, PeruState University of Mato Grosso do Sul (UEMS), Cassilândia 79540-000, MS, BrazilTecnológico Nacional de México/Instituto Tecnológico del Valle del Yaqui, Bácum 85270, Sonora, MexicoUniversidad de Oriente, Santiago de Cuba 90600, CubaOne of the diseases with the greatest economic impact on coffee cultivation worldwide and particularly in Peru is coffee rust (<i>Hemileia vastatrix</i>). The search for sustainable control methods as disease management strategies in coffee cultivation is necessary. The objective of this research was to determine the effectiveness of five biopesticides based on lemon verbena (<i>Cymbopogon citratus</i>) for the control of rust applied in laboratory and field conditions to allow the recovery of coffee (<i>Coffea arabica</i> L. var. typica) in La Convención, Cusco, Peru. Five biopesticides (oil, macerate, infusion, hydrolate and Biol) and four concentrations (0, 15, 20 and 25%) were evaluated. The biopesticides were evaluated under laboratory conditions (light and dark) at different concentrations. The design used was completely randomized in a factorial scheme. The biopesticides were incorporated into the culture medium and inoculated with 400 uredospores of rust, and the germination percentage was evaluated. Under field conditions, the biopesticides at the same concentrations were evaluated for 4 weeks after application. Under these field conditions, the incidence, severity and area under the disease progress curve (AUDPC) of selected plants with a natural degree of infection were evaluated. In the laboratory, the results showed that all biopesticides were effective in reducing the germination of rust uredospores to values <1% of germination in relation to the control, which showed values of 61% and 75% in the light and dark, respectively, independent of the concentration used, with no significant differences between them. In the field, 25% oil promoted the best response with values <1% and 0% incidence and severity, respectively, in the first two weeks after application. The AUDPC for this same treatment showed values of 7 in relation to 1595 of the control. <i>Cymbopogon citratus</i> oil is an excellent biopesticide that can be used to control coffee rust.https://www.mdpi.com/2223-7747/12/5/1166<i>Coffee arabica</i>lemon verbenasustainable control methodsuredospores |
| spellingShingle | Luis Morales-Aranibar Francisca Elena Yucra Yucra Nivia Marisol Pilares Estrada Policarpo Quispe Flores Renatto Nicolino Motta Zevallos Juan Carlos Llanos Zegarra Uriel Palomino Trujillo Carlos Genaro Morales Aranibar Hebert Hernán Soto Gonzales Jorge González Aguilera Leandris Argentel-Martínez Sucleidi Nápoles Vinent Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions Plants <i>Coffee arabica</i> lemon verbena sustainable control methods uredospores |
| title | Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions |
| title_full | Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions |
| title_fullStr | Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions |
| title_full_unstemmed | Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions |
| title_short | Production of New Biopesticides from <i>Cymbopogon citratus</i> for the Control of Coffee Rust (<i>Hemileia vastatrix</i>) under Laboratory and Field Conditions |
| title_sort | production of new biopesticides from i cymbopogon citratus i for the control of coffee rust i hemileia vastatrix i under laboratory and field conditions |
| topic | <i>Coffee arabica</i> lemon verbena sustainable control methods uredospores |
| url | https://www.mdpi.com/2223-7747/12/5/1166 |
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