The microbiome of the middle meatus in healthy adults.
Rhinitis and rhinosinusitis are multifactorial disease processes in which bacteria may play a role either in infection or stimulation of the inflammatory process. Rhinosinusitis has been historically studied with culture-based techniques, which have implicated several common pathogens in disease sta...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3875580?pdf=render |
| _version_ | 1819161219702980608 |
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| author | Vijay R Ramakrishnan Leah M Feazel Sarah A Gitomer Diana Ir Charles E Robertson Daniel N Frank |
| author_facet | Vijay R Ramakrishnan Leah M Feazel Sarah A Gitomer Diana Ir Charles E Robertson Daniel N Frank |
| author_sort | Vijay R Ramakrishnan |
| collection | DOAJ |
| description | Rhinitis and rhinosinusitis are multifactorial disease processes in which bacteria may play a role either in infection or stimulation of the inflammatory process. Rhinosinusitis has been historically studied with culture-based techniques, which have implicated several common pathogens in disease states. More recently, the NIH Human Microbiome Project has examined the microbiome at a number of accessible body sites, and demonstrated differences among healthy and diseased patients. Recent DNA-based sinus studies have suggested that healthy sinuses are not sterile, as was previously believed, but the normal sinonasal microbiome has yet to be thoroughly examined. Middle meatus swab specimens were collected from 28 consecutive patients presenting with no signs or symptoms of rhinosinusitis. Bacterial colonization was assessed in these specimens using quantitative PCR and 16S rRNA pyrosequencing. All subjects were positive for bacterial colonization of the middle meatus. Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acnes were the most prevalent and abundant microorganisms detected. Rich and diverse bacterial assemblages are present in the sinonasal cavity in the normal state, including opportunistic pathogens typically found in the nasopharynx. This work helps establish a baseline for understanding how the sinonasal microbiome may impact diseases of the upper airways. |
| first_indexed | 2024-12-22T17:08:52Z |
| format | Article |
| id | doaj.art-32b0a2eb1c054bbb84903a34560ebe71 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T17:08:52Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-32b0a2eb1c054bbb84903a34560ebe712022-12-21T18:19:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-01812e8550710.1371/journal.pone.0085507The microbiome of the middle meatus in healthy adults.Vijay R RamakrishnanLeah M FeazelSarah A GitomerDiana IrCharles E RobertsonDaniel N FrankRhinitis and rhinosinusitis are multifactorial disease processes in which bacteria may play a role either in infection or stimulation of the inflammatory process. Rhinosinusitis has been historically studied with culture-based techniques, which have implicated several common pathogens in disease states. More recently, the NIH Human Microbiome Project has examined the microbiome at a number of accessible body sites, and demonstrated differences among healthy and diseased patients. Recent DNA-based sinus studies have suggested that healthy sinuses are not sterile, as was previously believed, but the normal sinonasal microbiome has yet to be thoroughly examined. Middle meatus swab specimens were collected from 28 consecutive patients presenting with no signs or symptoms of rhinosinusitis. Bacterial colonization was assessed in these specimens using quantitative PCR and 16S rRNA pyrosequencing. All subjects were positive for bacterial colonization of the middle meatus. Staphylococcus aureus, Staphylococcus epidermidis and Propionibacterium acnes were the most prevalent and abundant microorganisms detected. Rich and diverse bacterial assemblages are present in the sinonasal cavity in the normal state, including opportunistic pathogens typically found in the nasopharynx. This work helps establish a baseline for understanding how the sinonasal microbiome may impact diseases of the upper airways.http://europepmc.org/articles/PMC3875580?pdf=render |
| spellingShingle | Vijay R Ramakrishnan Leah M Feazel Sarah A Gitomer Diana Ir Charles E Robertson Daniel N Frank The microbiome of the middle meatus in healthy adults. PLoS ONE |
| title | The microbiome of the middle meatus in healthy adults. |
| title_full | The microbiome of the middle meatus in healthy adults. |
| title_fullStr | The microbiome of the middle meatus in healthy adults. |
| title_full_unstemmed | The microbiome of the middle meatus in healthy adults. |
| title_short | The microbiome of the middle meatus in healthy adults. |
| title_sort | microbiome of the middle meatus in healthy adults |
| url | http://europepmc.org/articles/PMC3875580?pdf=render |
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