Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative
In this study, boroxine derivative (K2[B3O3F4OH]) was tested as an inhibitor of horseradish peroxidase (HRP) by spectrophotometric and electrochemical methods. The activity of horseradish peroxidase was first studied under steady-state kinetic conditions by a spectrophotometric method which required...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2017-07-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/22/7/1120 |
| _version_ | 1817998366405033984 |
|---|---|
| author | Jelena Ostojić Safija Herenda Zerina Bešić Mladen Miloš Borivoj Galić |
| author_facet | Jelena Ostojić Safija Herenda Zerina Bešić Mladen Miloš Borivoj Galić |
| author_sort | Jelena Ostojić |
| collection | DOAJ |
| description | In this study, boroxine derivative (K2[B3O3F4OH]) was tested as an inhibitor of horseradish peroxidase (HRP) by spectrophotometric and electrochemical methods. The activity of horseradish peroxidase was first studied under steady-state kinetic conditions by a spectrophotometric method which required the use of guaiacol as a second substrate to measure guaiacol peroxidation. The results of this method have shown that, by changing the concentration of guaiacol as the literature suggests, a different type of inhibition is observed than when changing the concentration of hydrogen peroxide as the substrate. This suggests that guaiacol interferes with the reaction in some way. The electrochemical method involves direct electron transfer of HRP immobilized in Nafion nanocomposite films on a glassy carbon (GC) electrode, creating a sensor with an electro-catalytic response to the reduction of hydrogen peroxide. The electrochemical method simplifies kinetic assays by removing the requirement of reducing substrates. |
| first_indexed | 2024-04-14T02:52:10Z |
| format | Article |
| id | doaj.art-32b17e1637894a30984fd13583f14703 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-04-14T02:52:10Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-32b17e1637894a30984fd13583f147032022-12-22T02:16:14ZengMDPI AGMolecules1420-30492017-07-01227112010.3390/molecules22071120molecules22071120Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine DerivativeJelena Ostojić0Safija Herenda1Zerina Bešić2Mladen Miloš3Borivoj Galić4Department of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaDepartment of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaDepartment of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaFaculty of Chemistry and Technology, University of Split, Teslina 10, 21 000 Split, CroatiaDepartment of Chemistry, Faculty of Science, University of Sarajevo, Zmaja od Bosne 33-35, 71 000 Sarajevo, Bosnia and HerzegovinaIn this study, boroxine derivative (K2[B3O3F4OH]) was tested as an inhibitor of horseradish peroxidase (HRP) by spectrophotometric and electrochemical methods. The activity of horseradish peroxidase was first studied under steady-state kinetic conditions by a spectrophotometric method which required the use of guaiacol as a second substrate to measure guaiacol peroxidation. The results of this method have shown that, by changing the concentration of guaiacol as the literature suggests, a different type of inhibition is observed than when changing the concentration of hydrogen peroxide as the substrate. This suggests that guaiacol interferes with the reaction in some way. The electrochemical method involves direct electron transfer of HRP immobilized in Nafion nanocomposite films on a glassy carbon (GC) electrode, creating a sensor with an electro-catalytic response to the reduction of hydrogen peroxide. The electrochemical method simplifies kinetic assays by removing the requirement of reducing substrates.https://www.mdpi.com/1420-3049/22/7/1120boroxine derivativeperoxidaseenzyme kineticscyclic voltammetry |
| spellingShingle | Jelena Ostojić Safija Herenda Zerina Bešić Mladen Miloš Borivoj Galić Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative Molecules boroxine derivative peroxidase enzyme kinetics cyclic voltammetry |
| title | Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative |
| title_full | Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative |
| title_fullStr | Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative |
| title_full_unstemmed | Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative |
| title_short | Advantages of an Electrochemical Method Compared to the Spectrophotometric Kinetic Study of Peroxidase Inhibition by Boroxine Derivative |
| title_sort | advantages of an electrochemical method compared to the spectrophotometric kinetic study of peroxidase inhibition by boroxine derivative |
| topic | boroxine derivative peroxidase enzyme kinetics cyclic voltammetry |
| url | https://www.mdpi.com/1420-3049/22/7/1120 |
| work_keys_str_mv | AT jelenaostojic advantagesofanelectrochemicalmethodcomparedtothespectrophotometrickineticstudyofperoxidaseinhibitionbyboroxinederivative AT safijaherenda advantagesofanelectrochemicalmethodcomparedtothespectrophotometrickineticstudyofperoxidaseinhibitionbyboroxinederivative AT zerinabesic advantagesofanelectrochemicalmethodcomparedtothespectrophotometrickineticstudyofperoxidaseinhibitionbyboroxinederivative AT mladenmilos advantagesofanelectrochemicalmethodcomparedtothespectrophotometrickineticstudyofperoxidaseinhibitionbyboroxinederivative AT borivojgalic advantagesofanelectrochemicalmethodcomparedtothespectrophotometrickineticstudyofperoxidaseinhibitionbyboroxinederivative |