Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes
Strontium (Sr) is a trace element found mainly in bone, and it performs a dual action by promoting bone formation and inhibiting bone resorption. Sr has been used to evaluate the gastrointestinal calcium (Ca) absorption capacity of dairy cows due to the similar physicochemical properties of the two...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-04-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/13/8/1301 |
| _version_ | 1797606693569298432 |
|---|---|
| author | Fangyuan Zeng Lan Li Jiaqi Yang Siqi Liu Yang Yuan Chenxu Zhao Jianguo Wang |
| author_facet | Fangyuan Zeng Lan Li Jiaqi Yang Siqi Liu Yang Yuan Chenxu Zhao Jianguo Wang |
| author_sort | Fangyuan Zeng |
| collection | DOAJ |
| description | Strontium (Sr) is a trace element found mainly in bone, and it performs a dual action by promoting bone formation and inhibiting bone resorption. Sr has been used to evaluate the gastrointestinal calcium (Ca) absorption capacity of dairy cows due to the similar physicochemical properties of the two elements. However, the possible effects of Sr on dairy cows remain unclear. This study aimed to explore the potential regulatory mechanism of Sr in bovine chondrocytes by performing transcriptomic and proteomic analyses. A total of 111 genes (52 up-regulated and 59 down-regulated) were identified as significantly altered (1.2-fold change and <i>p</i> < 0.05) between control and Sr-treated groups. Moreover, LC-MS-based proteomic analysis detected 286 changed proteins (159 up-regulated and 127 down-regulated) between the control and Sr-treated groups (1.2-fold change and <i>p</i> < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations of a combination analysis of the transcriptomic and proteomic data revealed that the genes were predominantly involved in chondrocyte proliferation and differentiation, fat metabolism, the inflammation process, and immune responses. Overall, our data reveal a potential regulatory mechanism of strontium in bovine chondrocytes, thus providing further insights into the functions and application of Sr in ruminants. |
| first_indexed | 2024-03-11T05:18:41Z |
| format | Article |
| id | doaj.art-32b1caa0897043ee93a3aff0fc8706fa |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T05:18:41Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-32b1caa0897043ee93a3aff0fc8706fa2023-11-17T17:59:50ZengMDPI AGAnimals2076-26152023-04-01138130110.3390/ani13081301Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine ChondrocytesFangyuan Zeng0Lan Li1Jiaqi Yang2Siqi Liu3Yang Yuan4Chenxu Zhao5Jianguo Wang6College of Veterinary Medicine, Northwest A&F University, Xianyang 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Xianyang 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Xianyang 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Xianyang 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Xianyang 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Xianyang 712100, ChinaCollege of Veterinary Medicine, Northwest A&F University, Xianyang 712100, ChinaStrontium (Sr) is a trace element found mainly in bone, and it performs a dual action by promoting bone formation and inhibiting bone resorption. Sr has been used to evaluate the gastrointestinal calcium (Ca) absorption capacity of dairy cows due to the similar physicochemical properties of the two elements. However, the possible effects of Sr on dairy cows remain unclear. This study aimed to explore the potential regulatory mechanism of Sr in bovine chondrocytes by performing transcriptomic and proteomic analyses. A total of 111 genes (52 up-regulated and 59 down-regulated) were identified as significantly altered (1.2-fold change and <i>p</i> < 0.05) between control and Sr-treated groups. Moreover, LC-MS-based proteomic analysis detected 286 changed proteins (159 up-regulated and 127 down-regulated) between the control and Sr-treated groups (1.2-fold change and <i>p</i> < 0.05). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations of a combination analysis of the transcriptomic and proteomic data revealed that the genes were predominantly involved in chondrocyte proliferation and differentiation, fat metabolism, the inflammation process, and immune responses. Overall, our data reveal a potential regulatory mechanism of strontium in bovine chondrocytes, thus providing further insights into the functions and application of Sr in ruminants.https://www.mdpi.com/2076-2615/13/8/1301strontiumbovine chondrocytetranscriptomicproteomics |
| spellingShingle | Fangyuan Zeng Lan Li Jiaqi Yang Siqi Liu Yang Yuan Chenxu Zhao Jianguo Wang Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes Animals strontium bovine chondrocyte transcriptomic proteomics |
| title | Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes |
| title_full | Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes |
| title_fullStr | Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes |
| title_full_unstemmed | Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes |
| title_short | Transcriptomic and Proteomic Analyses Reveal New Insights into Regulatory Mechanisms of Strontium in Bovine Chondrocytes |
| title_sort | transcriptomic and proteomic analyses reveal new insights into regulatory mechanisms of strontium in bovine chondrocytes |
| topic | strontium bovine chondrocyte transcriptomic proteomics |
| url | https://www.mdpi.com/2076-2615/13/8/1301 |
| work_keys_str_mv | AT fangyuanzeng transcriptomicandproteomicanalysesrevealnewinsightsintoregulatorymechanismsofstrontiuminbovinechondrocytes AT lanli transcriptomicandproteomicanalysesrevealnewinsightsintoregulatorymechanismsofstrontiuminbovinechondrocytes AT jiaqiyang transcriptomicandproteomicanalysesrevealnewinsightsintoregulatorymechanismsofstrontiuminbovinechondrocytes AT siqiliu transcriptomicandproteomicanalysesrevealnewinsightsintoregulatorymechanismsofstrontiuminbovinechondrocytes AT yangyuan transcriptomicandproteomicanalysesrevealnewinsightsintoregulatorymechanismsofstrontiuminbovinechondrocytes AT chenxuzhao transcriptomicandproteomicanalysesrevealnewinsightsintoregulatorymechanismsofstrontiuminbovinechondrocytes AT jianguowang transcriptomicandproteomicanalysesrevealnewinsightsintoregulatorymechanismsofstrontiuminbovinechondrocytes |