Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult Rats
We tested the influence of erythropoietin (EPO), a basic cytokine in erythropoiesis regulation, on the process of motor function and cognition after focal brain ischemia induced by a local application of endothelin. Endothelin-1 (ET-1) induced short lasting strong vasoconstriction, with described im...
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| Format: | Article |
| Language: | English |
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Karolinum Press
2014-01-01
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| Series: | Prague Medical Report |
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| Online Access: | https://pmr.lf1.cuni.cz/115/1/0005/ |
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| author | Michaela Hralová Eva Plaňanská Yvona Angerová Andrea Jadwiszczoková Jana Bortelová Marcela Lippertová-Grünerová Dana Marešová |
| author_facet | Michaela Hralová Eva Plaňanská Yvona Angerová Andrea Jadwiszczoková Jana Bortelová Marcela Lippertová-Grünerová Dana Marešová |
| author_sort | Michaela Hralová |
| collection | DOAJ |
| description | We tested the influence of erythropoietin (EPO), a basic cytokine in erythropoiesis regulation, on the process of motor function and cognition after focal brain ischemia induced by a local application of endothelin. Endothelin-1 (ET-1) induced short lasting strong vasoconstriction, with described impact on the structure and on the function of neuronal cells. Neurological description of motor function and Morris water maze test (the swimming test is one of most widely used methods for studying cognitive functions in rodents) were used to study the process of learning and memory in three-month-old male albino Wistar rats (n=52). Both tests were performed one week before, and three weeks after ischemia induction (endothelin application on the cortex in the area of a. cerebri media dx.). Experimental group received i.p. injection of EPO (5,000 IU/kg body weight, 10 min before endothelin application). Control group of animals received one i.p. injection of saline at the dose of 1 ml/kg body weight at the same time. Only sham surgery was performed in the third group of animals. Rats with EPO pretreatment before the experimental lesion exhibited significantly better motor and cognitive function then those with saline injection. No significant changes in the motor and cognitive function were found in the third group of rats (sham operated controls). |
| first_indexed | 2024-12-11T09:04:00Z |
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| id | doaj.art-32b2166e1f6e424fa561fe816696695f |
| institution | Directory Open Access Journal |
| issn | 1214-6994 2336-2936 |
| language | English |
| last_indexed | 2024-12-11T09:04:00Z |
| publishDate | 2014-01-01 |
| publisher | Karolinum Press |
| record_format | Article |
| series | Prague Medical Report |
| spelling | doaj.art-32b2166e1f6e424fa561fe816696695f2022-12-22T01:13:41ZengKarolinum PressPrague Medical Report1214-69942336-29362014-01-01115151510.14712/23362936.2014.1Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult RatsMichaela Hralová0Eva Plaňanská1Yvona Angerová2Andrea Jadwiszczoková3Jana Bortelová4Marcela Lippertová-Grünerová5Dana Marešová6Institute of Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Neurology, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicDepartment of Rehabilitation Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech RepublicInstitute of Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicInstitute of Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicDepartment of Rehabilitation Medicine, First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic; Faculty of Medicine, University of Cologne, Cologne, GermanyInstitute of Physiology, First Faculty of Medicine, Charles University in Prague, Prague, Czech RepublicWe tested the influence of erythropoietin (EPO), a basic cytokine in erythropoiesis regulation, on the process of motor function and cognition after focal brain ischemia induced by a local application of endothelin. Endothelin-1 (ET-1) induced short lasting strong vasoconstriction, with described impact on the structure and on the function of neuronal cells. Neurological description of motor function and Morris water maze test (the swimming test is one of most widely used methods for studying cognitive functions in rodents) were used to study the process of learning and memory in three-month-old male albino Wistar rats (n=52). Both tests were performed one week before, and three weeks after ischemia induction (endothelin application on the cortex in the area of a. cerebri media dx.). Experimental group received i.p. injection of EPO (5,000 IU/kg body weight, 10 min before endothelin application). Control group of animals received one i.p. injection of saline at the dose of 1 ml/kg body weight at the same time. Only sham surgery was performed in the third group of animals. Rats with EPO pretreatment before the experimental lesion exhibited significantly better motor and cognitive function then those with saline injection. No significant changes in the motor and cognitive function were found in the third group of rats (sham operated controls).https://pmr.lf1.cuni.cz/115/1/0005/RatsEndothelinErythropoietinFocal brain ischemiaMotor and cognitive function |
| spellingShingle | Michaela Hralová Eva Plaňanská Yvona Angerová Andrea Jadwiszczoková Jana Bortelová Marcela Lippertová-Grünerová Dana Marešová Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult Rats Prague Medical Report Rats Endothelin Erythropoietin Focal brain ischemia Motor and cognitive function |
| title | Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult Rats |
| title_full | Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult Rats |
| title_fullStr | Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult Rats |
| title_full_unstemmed | Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult Rats |
| title_short | Effects of a Single Dose of Erythropoietin on Motor Function and Cognition after Focal Brain Ischemia in Adult Rats |
| title_sort | effects of a single dose of erythropoietin on motor function and cognition after focal brain ischemia in adult rats |
| topic | Rats Endothelin Erythropoietin Focal brain ischemia Motor and cognitive function |
| url | https://pmr.lf1.cuni.cz/115/1/0005/ |
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