A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro
In this paper we describe the results obtained with a novel method to prepare depositions of asbestos fibres for toxicological tests in vitro. The technique is based on a micro-dispenser, working as an inkjet printer, able to deposit micro-sized droplets from a suspension of fibres in a liquid mediu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1116463/full |
| _version_ | 1811164288739442688 |
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| author | Giancarlo Della Ventura Giancarlo Della Ventura Giancarlo Della Ventura Ahmad Rabiee Augusto Marcelli Augusto Marcelli Salvatore Macis Annalisa D’Arco Gianluca Iezzi Francesco Radica Federico Lucci |
| author_facet | Giancarlo Della Ventura Giancarlo Della Ventura Giancarlo Della Ventura Ahmad Rabiee Augusto Marcelli Augusto Marcelli Salvatore Macis Annalisa D’Arco Gianluca Iezzi Francesco Radica Federico Lucci |
| author_sort | Giancarlo Della Ventura |
| collection | DOAJ |
| description | In this paper we describe the results obtained with a novel method to prepare depositions of asbestos fibres for toxicological tests in vitro. The technique is based on a micro-dispenser, working as an inkjet printer, able to deposit micro-sized droplets from a suspension of fibres in a liquid medium; we used here a highly evaporating liquid (ethanol) to reduce the experimental time, however other solvents could be used. Both the amount and spatial distribution of fibres on the substrate can be controlled by adjusting the parameters of the micro-dispenser such as deposition area, deposition time, uniformity and volume of the deposited liquid. Statistical analysis of images obtained by optical and scanning electron microscopy shows that this technique produces an extremely homogeneous distribution of fibers. Specifically, the number of deposited single fibres is maximized (up to 20 times), a feature that is essential when performing viability tests where agglomerated or untangled fibrous particles need to be avoided. |
| first_indexed | 2024-04-10T15:19:09Z |
| format | Article |
| id | doaj.art-32b9ef5f25ca4bdc9a27b247213ed2ed |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-10T15:19:09Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-32b9ef5f25ca4bdc9a27b247213ed2ed2023-02-14T16:42:13ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-02-011110.3389/fchem.2023.11164631116463A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitroGiancarlo Della Ventura0Giancarlo Della Ventura1Giancarlo Della Ventura2Ahmad Rabiee3Augusto Marcelli4Augusto Marcelli5Salvatore Macis6Annalisa D’Arco7Gianluca Iezzi8Francesco Radica9Federico Lucci10Department of Science, Roma Tre University, Rome, ItalyINFN, National Institute of Nuclear Physics, Frascati, ItalyINGV, National Institute of Geophysics and Volcanology, Rome, ItalyDepartment of Science, Roma Tre University, Rome, ItalyINFN, National Institute of Nuclear Physics, Frascati, ItalyRICMASS, Rome International Center for Materials Science Superstripes, Rome, ItalyDepartment of Physics, Sapienza University of Rome, Rome, ItalyDepartment of Physics, Sapienza University of Rome, Rome, ItalyDepartment of Engineering and Geology, University of Chieti, Rome, ItalyDepartment of Engineering and Geology, University of Chieti, Rome, ItalyDepartment of Earth and Geoenvironmental Sciences, University of Bari, Bari, ItalyIn this paper we describe the results obtained with a novel method to prepare depositions of asbestos fibres for toxicological tests in vitro. The technique is based on a micro-dispenser, working as an inkjet printer, able to deposit micro-sized droplets from a suspension of fibres in a liquid medium; we used here a highly evaporating liquid (ethanol) to reduce the experimental time, however other solvents could be used. Both the amount and spatial distribution of fibres on the substrate can be controlled by adjusting the parameters of the micro-dispenser such as deposition area, deposition time, uniformity and volume of the deposited liquid. Statistical analysis of images obtained by optical and scanning electron microscopy shows that this technique produces an extremely homogeneous distribution of fibers. Specifically, the number of deposited single fibres is maximized (up to 20 times), a feature that is essential when performing viability tests where agglomerated or untangled fibrous particles need to be avoided.https://www.frontiersin.org/articles/10.3389/fchem.2023.1116463/fullasbestos fibresdepositiontoxicological experimentsmicrodrop methodoptical and SEM imagesimage processing |
| spellingShingle | Giancarlo Della Ventura Giancarlo Della Ventura Giancarlo Della Ventura Ahmad Rabiee Augusto Marcelli Augusto Marcelli Salvatore Macis Annalisa D’Arco Gianluca Iezzi Francesco Radica Federico Lucci A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro Frontiers in Chemistry asbestos fibres deposition toxicological experiments microdrop method optical and SEM images image processing |
| title | A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro |
| title_full | A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro |
| title_fullStr | A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro |
| title_full_unstemmed | A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro |
| title_short | A new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro |
| title_sort | new approach to deposit homogeneous samples of asbestos fibres for toxicological tests in vitro |
| topic | asbestos fibres deposition toxicological experiments microdrop method optical and SEM images image processing |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1116463/full |
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