The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test
IntroductionAutism spectrum disorder (ASD) is characterized by deficits in communication and social interaction, restricted interests, repetitive behaviors, and sensory alterations, with auditory hypersensitivity being one of the most commonly reported sensory–perceptual abnormalities. Several candi...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-08-01
|
| Series: | Frontiers in Behavioral Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1205507/full |
| _version_ | 1797737633583988736 |
|---|---|
| author | Ana Margarida Gonçalves Ana Margarida Gonçalves Nuno Sousa Nuno Sousa Luis Jacinto Patricia Monteiro Patricia Monteiro Patricia Monteiro |
| author_facet | Ana Margarida Gonçalves Ana Margarida Gonçalves Nuno Sousa Nuno Sousa Luis Jacinto Patricia Monteiro Patricia Monteiro Patricia Monteiro |
| author_sort | Ana Margarida Gonçalves |
| collection | DOAJ |
| description | IntroductionAutism spectrum disorder (ASD) is characterized by deficits in communication and social interaction, restricted interests, repetitive behaviors, and sensory alterations, with auditory hypersensitivity being one of the most commonly reported sensory–perceptual abnormalities. Several candidate genes for involvement in this disorder have emerged from patient studies, including SHANK3, a gene that encodes a protein (SHANK3) in the postsynaptic density of excitatory synapses. Previous work has shown that mutant mice carrying a human ASD mutation in the Shank3 gene (InsG3680) exhibit repetitive behaviors and social interaction deficits, indicating important construct and face validity for this genotype as an animal model of ASD.MethodsTo further address whether these mice also present auditory sensory–perceptual alterations, we developed a novel behavioral test in which mice can choose between different soundscapes.ResultsOur results reveal that, in comparison to wild-type mice, Shank3 mutants display a strong behavioral preference toward silent regions of the arena.DiscussionThese data suggest that Shank3- mutant mice might express an auditory hypersensitivity phenotype, further adding to the face validity of this genotype as an animal model of ASD. |
| first_indexed | 2024-03-12T13:31:16Z |
| format | Article |
| id | doaj.art-32ba25a5fe7046ea8c2e44a04faa5729 |
| institution | Directory Open Access Journal |
| issn | 1662-5153 |
| language | English |
| last_indexed | 2024-03-12T13:31:16Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Behavioral Neuroscience |
| spelling | doaj.art-32ba25a5fe7046ea8c2e44a04faa57292023-08-24T10:22:11ZengFrontiers Media S.A.Frontiers in Behavioral Neuroscience1662-51532023-08-011710.3389/fnbeh.2023.12055071205507The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral testAna Margarida Gonçalves0Ana Margarida Gonçalves1Nuno Sousa2Nuno Sousa3Luis Jacinto4Patricia Monteiro5Patricia Monteiro6Patricia Monteiro7Life and Health Sciences Research Institute, School of Medicine, University of Minho, Braga, PortugalICVS/3B’s—PT Government Associate Laboratory, Braga/Guimarães, PortugalLife and Health Sciences Research Institute, School of Medicine, University of Minho, Braga, PortugalICVS/3B’s—PT Government Associate Laboratory, Braga/Guimarães, PortugalExperimental Biology Unit, Department of Biomedicine, Faculty of Medicine, University of Porto (FMUP), Porto, PortugalLife and Health Sciences Research Institute, School of Medicine, University of Minho, Braga, PortugalICVS/3B’s—PT Government Associate Laboratory, Braga/Guimarães, PortugalExperimental Biology Unit, Department of Biomedicine, Faculty of Medicine, University of Porto (FMUP), Porto, PortugalIntroductionAutism spectrum disorder (ASD) is characterized by deficits in communication and social interaction, restricted interests, repetitive behaviors, and sensory alterations, with auditory hypersensitivity being one of the most commonly reported sensory–perceptual abnormalities. Several candidate genes for involvement in this disorder have emerged from patient studies, including SHANK3, a gene that encodes a protein (SHANK3) in the postsynaptic density of excitatory synapses. Previous work has shown that mutant mice carrying a human ASD mutation in the Shank3 gene (InsG3680) exhibit repetitive behaviors and social interaction deficits, indicating important construct and face validity for this genotype as an animal model of ASD.MethodsTo further address whether these mice also present auditory sensory–perceptual alterations, we developed a novel behavioral test in which mice can choose between different soundscapes.ResultsOur results reveal that, in comparison to wild-type mice, Shank3 mutants display a strong behavioral preference toward silent regions of the arena.DiscussionThese data suggest that Shank3- mutant mice might express an auditory hypersensitivity phenotype, further adding to the face validity of this genotype as an animal model of ASD.https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1205507/fullautism spectrum disorder (ASD)Shank3auditory hypersensitivitybehaviorsensory alterationsanimal model |
| spellingShingle | Ana Margarida Gonçalves Ana Margarida Gonçalves Nuno Sousa Nuno Sousa Luis Jacinto Patricia Monteiro Patricia Monteiro Patricia Monteiro The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test Frontiers in Behavioral Neuroscience autism spectrum disorder (ASD) Shank3 auditory hypersensitivity behavior sensory alterations animal model |
| title | The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test |
| title_full | The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test |
| title_fullStr | The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test |
| title_full_unstemmed | The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test |
| title_short | The Shank3-InsG3680(+/+) mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test |
| title_sort | shank3 insg3680 mouse model of autism spectrum disorder displays auditory avoidance in a novel behavioral test |
| topic | autism spectrum disorder (ASD) Shank3 auditory hypersensitivity behavior sensory alterations animal model |
| url | https://www.frontiersin.org/articles/10.3389/fnbeh.2023.1205507/full |
| work_keys_str_mv | AT anamargaridagoncalves theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT anamargaridagoncalves theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT nunosousa theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT nunosousa theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT luisjacinto theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT patriciamonteiro theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT patriciamonteiro theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT patriciamonteiro theshank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT anamargaridagoncalves shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT anamargaridagoncalves shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT nunosousa shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT nunosousa shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT luisjacinto shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT patriciamonteiro shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT patriciamonteiro shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest AT patriciamonteiro shank3insg3680mousemodelofautismspectrumdisorderdisplaysauditoryavoidanceinanovelbehavioraltest |