Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis
Leishmania is the unicellular parasite transmitted by phlebotomine sand fly bite. It exists in two different forms; extracellular promastigotes, occurring in the gut of sand flies, and intracellular, round-shaped amastigotes residing mainly in vertebrate macrophages. As amastigotes originating from...
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1022448/full |
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| author | Lenka Pacakova Karel Harant Karel Harant Petr Volf Tereza Lestinova |
| author_facet | Lenka Pacakova Karel Harant Karel Harant Petr Volf Tereza Lestinova |
| author_sort | Lenka Pacakova |
| collection | DOAJ |
| description | Leishmania is the unicellular parasite transmitted by phlebotomine sand fly bite. It exists in two different forms; extracellular promastigotes, occurring in the gut of sand flies, and intracellular, round-shaped amastigotes residing mainly in vertebrate macrophages. As amastigotes originating from infected animals are often present in insufficient quality and quantity, two alternative types of amastigotes were introduced for laboratory experiments: axenic amastigotes and amastigotes from macrophages infected in vitro. Nevertheless, there is very little information about the degree of similarity/difference among these three types of amastigotes on proteomic level, whose comparison is crucial for assessing the suitability of using alternative types of amastigotes in experiments. In this study, L. mexicana amastigotes obtained from lesion of infected BALB/c mice were proteomically compared with alternatively cultivated amastigotes (axenic and macrophage-derived ones). Amastigotes of all three types were isolated, individually treated and analysed by LC-MS/MS proteomic analysis with quantification using TMT10-plex isobaric labeling. Significant differences were observed in the abundance of metabolic enzymes, virulence factors and proteins involved in translation and condensation of DNA. The most pronounced differences were observed between axenic amastigotes and lesion-derived amastigotes, macrophage-derived amastigotes were mostly intermediate between axenic and lesion-derived ones. |
| first_indexed | 2024-04-11T17:30:55Z |
| format | Article |
| id | doaj.art-32babd7070994cf09c8e44616a6e72f0 |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T17:30:55Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-32babd7070994cf09c8e44616a6e72f02022-12-22T04:12:02ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-11-011210.3389/fcimb.2022.10224481022448Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysisLenka Pacakova0Karel Harant1Karel Harant2Petr Volf3Tereza Lestinova4Department of Parasitology, Charles University, Prague, CzechiaBiotechnology and Biomedicine Centre of the Academy of Sciences and Charles University (BIOCEV), Vestec u Prahy, CzechiaProteomics Core Facility, Faculty of Science, Charles University, Prague, CzechiaDepartment of Parasitology, Charles University, Prague, CzechiaDepartment of Parasitology, Charles University, Prague, CzechiaLeishmania is the unicellular parasite transmitted by phlebotomine sand fly bite. It exists in two different forms; extracellular promastigotes, occurring in the gut of sand flies, and intracellular, round-shaped amastigotes residing mainly in vertebrate macrophages. As amastigotes originating from infected animals are often present in insufficient quality and quantity, two alternative types of amastigotes were introduced for laboratory experiments: axenic amastigotes and amastigotes from macrophages infected in vitro. Nevertheless, there is very little information about the degree of similarity/difference among these three types of amastigotes on proteomic level, whose comparison is crucial for assessing the suitability of using alternative types of amastigotes in experiments. In this study, L. mexicana amastigotes obtained from lesion of infected BALB/c mice were proteomically compared with alternatively cultivated amastigotes (axenic and macrophage-derived ones). Amastigotes of all three types were isolated, individually treated and analysed by LC-MS/MS proteomic analysis with quantification using TMT10-plex isobaric labeling. Significant differences were observed in the abundance of metabolic enzymes, virulence factors and proteins involved in translation and condensation of DNA. The most pronounced differences were observed between axenic amastigotes and lesion-derived amastigotes, macrophage-derived amastigotes were mostly intermediate between axenic and lesion-derived ones.https://www.frontiersin.org/articles/10.3389/fcimb.2022.1022448/fullproteomeLeishmania (L) mexicanaamastigoteaxenicmacrophagelesion |
| spellingShingle | Lenka Pacakova Karel Harant Karel Harant Petr Volf Tereza Lestinova Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis Frontiers in Cellular and Infection Microbiology proteome Leishmania (L) mexicana amastigote axenic macrophage lesion |
| title | Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis |
| title_full | Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis |
| title_fullStr | Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis |
| title_full_unstemmed | Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis |
| title_short | Three types of Leishmania mexicana amastigotes: Proteome comparison by quantitative proteomic analysis |
| title_sort | three types of leishmania mexicana amastigotes proteome comparison by quantitative proteomic analysis |
| topic | proteome Leishmania (L) mexicana amastigote axenic macrophage lesion |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.1022448/full |
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