Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies
Background: Lipopolysaccharide (LPS) administration is one of the most commonly used methods for inducing inflammation in animal models. Several animal studies have investigated the effects of acute and chronic peripheral administration of LPS on cognitive impairment. However, no previous study has...
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| Format: | Article |
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IMR Press
2022-03-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://www.imrpress.com/journal/JIN/21/2/10.31083/j.jin2102049 |
| _version_ | 1828278503904116736 |
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| author | Noor Ahmed Alzahrani Khulud Abdullah Bahaidrah Rasha Abdulrashed Mansouri Hadeil Muhanna Alsufiani Badrah Saeed Alghamdi |
| author_facet | Noor Ahmed Alzahrani Khulud Abdullah Bahaidrah Rasha Abdulrashed Mansouri Hadeil Muhanna Alsufiani Badrah Saeed Alghamdi |
| author_sort | Noor Ahmed Alzahrani |
| collection | DOAJ |
| description | Background: Lipopolysaccharide (LPS) administration is one of the most commonly used methods for inducing inflammation in animal models. Several animal studies have investigated the effects of acute and chronic peripheral administration of LPS on cognitive impairment. However, no previous study has compared the effects of different doses of chronically administered LPS on recognition memory performance. Aim: Here, we aimed to investigate the optimal dose of chronically administered LPS for the induction of recognition memory impairment in mice. Materials and methods: LPS at different doses (0.25, 0.50 and 0.75 mg/kg) was administered to SWR/J mice daily for 7 days. On day 9, the open field, novel object recognition and novel arm discrimination behavioral tests were performed. Additionally, prefrontal cortical histological examination was conducted. Results: Compared with the control group, mice injected with 0.75 mg/kg LPS notably showed no object preference (familiar vs. novel), a reduction in the discrimination index, and spatial recognition impairment. Administration of the 0.25 and 0.50 mg/kg doses of LPS showed a preference for the novel object compared with the familiar object, had no significant impact on the discrimination index, and caused spatial recognition impairment. These behavioral results are in line with the histological examination of the prefrontal cortex, which revealed that the 0.75 mg/kg dose produced the most histological damage. Conclusions: Our findings suggest that for chronic peripheral administration of LPS, 0.75 mg/kg is the optimal dose for inducing neuroinflammation-associated recognition memory deficits. |
| first_indexed | 2024-04-13T07:32:14Z |
| format | Article |
| id | doaj.art-32bbc322458e4c5b9d8e359c2e6632ea |
| institution | Directory Open Access Journal |
| issn | 0219-6352 |
| language | English |
| last_indexed | 2024-04-13T07:32:14Z |
| publishDate | 2022-03-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-32bbc322458e4c5b9d8e359c2e6632ea2022-12-22T02:56:19ZengIMR PressJournal of Integrative Neuroscience0219-63522022-03-0121204910.31083/j.jin2102049S0219-6352(22)00309-6Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studiesNoor Ahmed Alzahrani0Khulud Abdullah Bahaidrah1Rasha Abdulrashed Mansouri2Hadeil Muhanna Alsufiani3Badrah Saeed Alghamdi4Department of Biochemistry, Faculty of Sciences, King Abdulaziz University, 21589 Jeddah, Saudi ArabiaDepartment of Biochemistry, Faculty of Sciences, King Abdulaziz University, 21589 Jeddah, Saudi ArabiaDepartment of Biochemistry, Faculty of Sciences, King Abdulaziz University, 21589 Jeddah, Saudi ArabiaDepartment of Biochemistry, Faculty of Sciences, King Abdulaziz University, 21589 Jeddah, Saudi ArabiaDepartment of Physiology, Neuroscience Unit, Faculty of Medicine, King Abdulaziz University, Jeddah, Saudi ArabiaBackground: Lipopolysaccharide (LPS) administration is one of the most commonly used methods for inducing inflammation in animal models. Several animal studies have investigated the effects of acute and chronic peripheral administration of LPS on cognitive impairment. However, no previous study has compared the effects of different doses of chronically administered LPS on recognition memory performance. Aim: Here, we aimed to investigate the optimal dose of chronically administered LPS for the induction of recognition memory impairment in mice. Materials and methods: LPS at different doses (0.25, 0.50 and 0.75 mg/kg) was administered to SWR/J mice daily for 7 days. On day 9, the open field, novel object recognition and novel arm discrimination behavioral tests were performed. Additionally, prefrontal cortical histological examination was conducted. Results: Compared with the control group, mice injected with 0.75 mg/kg LPS notably showed no object preference (familiar vs. novel), a reduction in the discrimination index, and spatial recognition impairment. Administration of the 0.25 and 0.50 mg/kg doses of LPS showed a preference for the novel object compared with the familiar object, had no significant impact on the discrimination index, and caused spatial recognition impairment. These behavioral results are in line with the histological examination of the prefrontal cortex, which revealed that the 0.75 mg/kg dose produced the most histological damage. Conclusions: Our findings suggest that for chronic peripheral administration of LPS, 0.75 mg/kg is the optimal dose for inducing neuroinflammation-associated recognition memory deficits.https://www.imrpress.com/journal/JIN/21/2/10.31083/j.jin2102049lipopolysaccharideneuroinflammationrecognition memoryprefrontal cortexchronicalzheimer diseaseperipheral administrationrecognition memory lossnovel arm discrimination taskacute |
| spellingShingle | Noor Ahmed Alzahrani Khulud Abdullah Bahaidrah Rasha Abdulrashed Mansouri Hadeil Muhanna Alsufiani Badrah Saeed Alghamdi Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies Journal of Integrative Neuroscience lipopolysaccharide neuroinflammation recognition memory prefrontal cortex chronic alzheimer disease peripheral administration recognition memory loss novel arm discrimination task acute |
| title | Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies |
| title_full | Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies |
| title_fullStr | Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies |
| title_full_unstemmed | Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies |
| title_short | Investigation of the optimal dose for experimental lipopolysaccharide-induced recognition memory impairment: behavioral and histological studies |
| title_sort | investigation of the optimal dose for experimental lipopolysaccharide induced recognition memory impairment behavioral and histological studies |
| topic | lipopolysaccharide neuroinflammation recognition memory prefrontal cortex chronic alzheimer disease peripheral administration recognition memory loss novel arm discrimination task acute |
| url | https://www.imrpress.com/journal/JIN/21/2/10.31083/j.jin2102049 |
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