Isolation and Purifi cation of Chitinase Bacillus sp. D2 Isolated from Potato Rhizosfer
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| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada, Yogyakarta
2015-11-01
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| Series: | Indonesian Journal of Biotechnology |
| Online Access: | http://journal.ugm.ac.id/ijbiotech/article/view/7851 |
| _version_ | 1819154776897617920 |
|---|---|
| author | Sebastian Margino Chatarina Behar Widya Asmara |
| author_facet | Sebastian Margino Chatarina Behar Widya Asmara |
| author_sort | Sebastian Margino |
| collection | DOAJ |
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table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} --> <!--[endif] --> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">Potato Cyst Nematodes (</span><em><span style="font-size: 9.5pt; font-family: "BookAntiqua-Italic","serif";" lang="EN-GB">Globodera rostochiensis) </span></em><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">is one of the important potato’s pests and caused economic</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">looses up to 70% in the several centrals of potato plantations in Indonesia. Potato Cyst Nematodes (PCN) shell</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">component of egg shell containing chitin (inner layer) and vitelline/protein (outer layer), so the purpose of</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">research was to fi nd out of chitin degrading bacteria for controlling of egg’s PCN by cutting of their life cycle.</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">The results showed that </span><em><span style="font-size: 9.5pt; font-family: "BookAntiqua-Italic","serif";" lang="EN-GB">Bacillus </span></em><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">sp. D2 isolated from potato rhizosphere could produce extra cellular chitinase</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">in the medium containing of 0.20% colloidal chitin and fermented for 72 hours. Result of chitinase purifi cation</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">using ammonium sulphate precipitation and </span><em><span style="font-size: 9.5pt; font-family: "BookAntiqua-Italic","serif";" lang="EN-GB">DEAE-Cellulose </span></em><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">ion-exchange chromatography showed a specifi c</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">activity 2691,052 U/mg and analyzing using SDS-PAGE 12.5% resulted in molecular weight 30 kDa. The</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">apparent K</span><span style="font-size: 5.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">m </span><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">and V</span><span style="font-size: 5.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">max </span><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">of chitinase towards colloidal chitin were 2 mg/ml and 2.2 μg/h, respectively.</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB"> </span></p> |
| first_indexed | 2024-12-22T15:26:28Z |
| format | Article |
| id | doaj.art-32bbefda9f8e4068922611aaf68f7b70 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-12-22T15:26:28Z |
| publishDate | 2015-11-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-32bbefda9f8e4068922611aaf68f7b702022-12-21T18:21:28ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412015-11-011716567Isolation and Purifi cation of Chitinase Bacillus sp. 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--> <!--[endif] --><!--[if gte mso 10]> <mce:style><! /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-qformat:yes; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:11.0pt; font-family:"Calibri","sans-serif"; mso-ascii-font-family:Calibri; mso-ascii-theme-font:minor-latin; mso-fareast-font-family:"Times New Roman"; mso-fareast-theme-font:minor-fareast; mso-hansi-font-family:Calibri; mso-hansi-theme-font:minor-latin; mso-bidi-font-family:"Times New Roman"; mso-bidi-theme-font:minor-bidi;} --> <!--[endif] --> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">Potato Cyst Nematodes (</span><em><span style="font-size: 9.5pt; font-family: "BookAntiqua-Italic","serif";" lang="EN-GB">Globodera rostochiensis) </span></em><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">is one of the important potato’s pests and caused economic</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">looses up to 70% in the several centrals of potato plantations in Indonesia. Potato Cyst Nematodes (PCN) shell</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">component of egg shell containing chitin (inner layer) and vitelline/protein (outer layer), so the purpose of</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">research was to fi nd out of chitin degrading bacteria for controlling of egg’s PCN by cutting of their life cycle.</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">The results showed that </span><em><span style="font-size: 9.5pt; font-family: "BookAntiqua-Italic","serif";" lang="EN-GB">Bacillus </span></em><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">sp. D2 isolated from potato rhizosphere could produce extra cellular chitinase</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">in the medium containing of 0.20% colloidal chitin and fermented for 72 hours. Result of chitinase purifi cation</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">using ammonium sulphate precipitation and </span><em><span style="font-size: 9.5pt; font-family: "BookAntiqua-Italic","serif";" lang="EN-GB">DEAE-Cellulose </span></em><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">ion-exchange chromatography showed a specifi c</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">activity 2691,052 U/mg and analyzing using SDS-PAGE 12.5% resulted in molecular weight 30 kDa. The</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">apparent K</span><span style="font-size: 5.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">m </span><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">and V</span><span style="font-size: 5.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">max </span><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB">of chitinase towards colloidal chitin were 2 mg/ml and 2.2 μg/h, respectively.</span></p> <p class="MsoNormal"><span style="font-size: 9.5pt; font-family: "BookAntiqua","serif";" lang="EN-GB"> </span></p>http://journal.ugm.ac.id/ijbiotech/article/view/7851 |
| spellingShingle | Sebastian Margino Chatarina Behar Widya Asmara Isolation and Purifi cation of Chitinase Bacillus sp. D2 Isolated from Potato Rhizosfer Indonesian Journal of Biotechnology |
| title | Isolation and Purifi cation of Chitinase Bacillus sp. D2 Isolated from Potato Rhizosfer |
| title_full | Isolation and Purifi cation of Chitinase Bacillus sp. D2 Isolated from Potato Rhizosfer |
| title_fullStr | Isolation and Purifi cation of Chitinase Bacillus sp. D2 Isolated from Potato Rhizosfer |
| title_full_unstemmed | Isolation and Purifi cation of Chitinase Bacillus sp. D2 Isolated from Potato Rhizosfer |
| title_short | Isolation and Purifi cation of Chitinase Bacillus sp. D2 Isolated from Potato Rhizosfer |
| title_sort | isolation and purifi cation of chitinase bacillus sp d2 isolated from potato rhizosfer |
| url | http://journal.ugm.ac.id/ijbiotech/article/view/7851 |
| work_keys_str_mv | AT sebastianmargino isolationandpurificationofchitinasebacillusspd2isolatedfrompotatorhizosfer AT chatarinabehar isolationandpurificationofchitinasebacillusspd2isolatedfrompotatorhizosfer AT widyaasmara isolationandpurificationofchitinasebacillusspd2isolatedfrompotatorhizosfer |