The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized Rats
INTRODUCTION: Aim of this study was to determine the second phase changes that may occur after a splenectomy; when probiotics, with proven therapeutic effects of intestinal mucosal immunity and systemic immunity, are utilized. METHODS: Total of 56 female Sprague-Dawley rats were used and divided int...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Van Yuzuncu Yil University, School of Medicine
2020-09-01
|
| Series: | Van Tıp Dergisi |
| Subjects: | |
| Online Access: | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-92979 |
| _version_ | 1797421310071013376 |
|---|---|
| author | Mutlu Sahin Erkan Ozturk Mehmet Fatih Can Ismail Hakki Ozerhan Gokhan Yagci Belma Aslim |
| author_facet | Mutlu Sahin Erkan Ozturk Mehmet Fatih Can Ismail Hakki Ozerhan Gokhan Yagci Belma Aslim |
| author_sort | Mutlu Sahin |
| collection | DOAJ |
| description | INTRODUCTION: Aim of this study was to determine the second phase changes that may occur after a splenectomy; when probiotics, with proven therapeutic effects of intestinal mucosal immunity and systemic immunity, are utilized. METHODS: Total of 56 female Sprague-Dawley rats were used and divided into eight groups. Low exopolysaccharide (EPS) producing Lactobacillus delbrueckii subspecies bulgaricus A13 strain, or high EPS producing Lactobacillus delbrueckii subspecies bulgaricus B3 strain, was orally applied to the rats pre-operatively or post-operatively. Splenectomy was performed on the 8th day of the study and cardiac blood sampling and small bowel resection were performed on the 16th day. RESULTS: In terms of systemic immunity assay whilst there was no significant difference found between IL-6 and IL-10, a significant difference was detected between the groups in TNF-α, TGF-β, IL-4, IL-12 and IL-12 / IL-4 statistical comparisons (p <0.05).
A13 strain was observed to further increase TGF-β levels after a splenectomy, while further decreasing IL-4 levels. Also, A13 strain application in the splenectomized groups reduced TNF-α levels. TNF-α levels decreased in B3 strain treated groups; TGF-β elevation was found to be higher, and this strain reduced IL-12, IL-4 and IL-10. B3 strain was found to have a more depressing effect on IL-12 and TNF-α, than A13, in splenectomized groups.
DISCUSSION AND CONCLUSION: It has been determined that splenectomy leads to immunosuppression; that probiotics –especially the high EPS producing- have an indirect anti-inflammatory effect with cytokine regulation; and that with the cytokine profiles they exhibit, they sytematically support mucosal immunity. |
| first_indexed | 2024-03-09T07:15:02Z |
| format | Article |
| id | doaj.art-32bf0374e498434fa455cddd587f58d2 |
| institution | Directory Open Access Journal |
| issn | 2587-0351 |
| language | English |
| last_indexed | 2024-03-09T07:15:02Z |
| publishDate | 2020-09-01 |
| publisher | Van Yuzuncu Yil University, School of Medicine |
| record_format | Article |
| series | Van Tıp Dergisi |
| spelling | doaj.art-32bf0374e498434fa455cddd587f58d22023-12-03T08:39:30ZengVan Yuzuncu Yil University, School of MedicineVan Tıp Dergisi2587-03512020-09-0127438839610.5505/vtd.2020.92979VTD-92979The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized RatsMutlu Sahin0Erkan Ozturk1Mehmet Fatih Can2Ismail Hakki Ozerhan3Gokhan Yagci4Belma Aslim5Kecıoren Education And Research Hospital, General Surgery Service, AnkaraGulhane Military Medical Academy, AnkaraLokman Hekim University, Department of General Surgery, AnkaraLokman Hekim University, Department of General Surgery, AnkaraGulhane Military Medical Academy, AnkaraGazi University Faculty of Arts and Sciences, AnkaraINTRODUCTION: Aim of this study was to determine the second phase changes that may occur after a splenectomy; when probiotics, with proven therapeutic effects of intestinal mucosal immunity and systemic immunity, are utilized. METHODS: Total of 56 female Sprague-Dawley rats were used and divided into eight groups. Low exopolysaccharide (EPS) producing Lactobacillus delbrueckii subspecies bulgaricus A13 strain, or high EPS producing Lactobacillus delbrueckii subspecies bulgaricus B3 strain, was orally applied to the rats pre-operatively or post-operatively. Splenectomy was performed on the 8th day of the study and cardiac blood sampling and small bowel resection were performed on the 16th day. RESULTS: In terms of systemic immunity assay whilst there was no significant difference found between IL-6 and IL-10, a significant difference was detected between the groups in TNF-α, TGF-β, IL-4, IL-12 and IL-12 / IL-4 statistical comparisons (p <0.05). A13 strain was observed to further increase TGF-β levels after a splenectomy, while further decreasing IL-4 levels. Also, A13 strain application in the splenectomized groups reduced TNF-α levels. TNF-α levels decreased in B3 strain treated groups; TGF-β elevation was found to be higher, and this strain reduced IL-12, IL-4 and IL-10. B3 strain was found to have a more depressing effect on IL-12 and TNF-α, than A13, in splenectomized groups. DISCUSSION AND CONCLUSION: It has been determined that splenectomy leads to immunosuppression; that probiotics –especially the high EPS producing- have an indirect anti-inflammatory effect with cytokine regulation; and that with the cytokine profiles they exhibit, they sytematically support mucosal immunity.https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-92979splenectomyprobioticslactobacillus delbrueckiiimmunityimmunity mucosal |
| spellingShingle | Mutlu Sahin Erkan Ozturk Mehmet Fatih Can Ismail Hakki Ozerhan Gokhan Yagci Belma Aslim The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized Rats Van Tıp Dergisi splenectomy probiotics lactobacillus delbrueckii immunity immunity mucosal |
| title | The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized Rats |
| title_full | The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized Rats |
| title_fullStr | The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized Rats |
| title_full_unstemmed | The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized Rats |
| title_short | The Effects of Probiotics, on the Systemic and Intestinal Mucosal Immunity, of Splenectomized Rats |
| title_sort | effects of probiotics on the systemic and intestinal mucosal immunity of splenectomized rats |
| topic | splenectomy probiotics lactobacillus delbrueckii immunity immunity mucosal |
| url | https://jag.journalagent.com/z4/download_fulltext.asp?pdir=vtd&un=VTD-92979 |
| work_keys_str_mv | AT mutlusahin theeffectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT erkanozturk theeffectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT mehmetfatihcan theeffectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT ismailhakkiozerhan theeffectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT gokhanyagci theeffectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT belmaaslim theeffectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT mutlusahin effectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT erkanozturk effectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT mehmetfatihcan effectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT ismailhakkiozerhan effectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT gokhanyagci effectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats AT belmaaslim effectsofprobioticsonthesystemicandintestinalmucosalimmunityofsplenectomizedrats |