Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts
The quality and developmental capacity of oocytes derived from in vitro maturation (IVM) remain unsatisfactory, which greatly impairs the efficiency and application of embryo technologies. The present experiment was designed to investigate the effect of the supplementation of EGF, IGF-1, and Cx37 in...
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MDPI AG
2022-04-01
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| Online Access: | https://www.mdpi.com/2073-4425/13/5/805 |
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| author | Sha Yang Yuze Yang Haisheng Hao Weihua Du Yunwei Pang Shanjiang Zhao Huiying Zou Huabin Zhu Peipei Zhang Xueming Zhao |
| author_facet | Sha Yang Yuze Yang Haisheng Hao Weihua Du Yunwei Pang Shanjiang Zhao Huiying Zou Huabin Zhu Peipei Zhang Xueming Zhao |
| author_sort | Sha Yang |
| collection | DOAJ |
| description | The quality and developmental capacity of oocytes derived from in vitro maturation (IVM) remain unsatisfactory, which greatly impairs the efficiency and application of embryo technologies. The present experiment was designed to investigate the effect of the supplementation of EGF, IGF-1, and Cx37 in an IVM medium on the maturation quality and development ability of bovine oocytes. The cytoplasmic maturation events of oocytes and the quality of in vitro fertilization (IVF) blastocysts were examined to investigate the relative mechanisms. Our results showed that the nuclear maturation and blastocyst development after the IVF of oocytes treated with 25 μg/mL Cx37 or the combination of 50 ng/mL EGF and 100 ng/mL IGF-1 were significantly increased compared to those of the control group (<i>p</i> < 0.05). Furthermore, the blastocyst rate, and blastocyst total cell number and survival rate after vitrification of the EGF+IGF-1+Cx37 group, were significantly higher than those of the control group (<i>p</i> < 0.05), but lower than those of the FSH+LH+EGF+IGF-1+Cx37 group (<i>p</i> < 0.05). The transzonal projection (TZP) intensity, glutathione (GSH) level, and mitochondrial function of the EGF+IGF-1+Cx37 group were significantly higher than that of the control group, and lower than those of the FSH+LH+EGF+IGF-1+Cx37 group, in contrast to the results of the reactive oxygen species (ROS) levels. In conclusion, our results showed that the supplementation of 50 ng/mL EGF, 100 ng/mL IGF-1, and 25 μg/mL Cx37 in the IVM of bovine oocytes significantly improved their quality and developmental ability by increasing the TZP, mitochondrial function, and GSH level. |
| first_indexed | 2024-03-10T03:50:03Z |
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| language | English |
| last_indexed | 2024-03-10T03:50:03Z |
| publishDate | 2022-04-01 |
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| series | Genes |
| spelling | doaj.art-32c1068ee01a4d8fa524d6ced6705d342023-11-23T11:09:44ZengMDPI AGGenes2073-44252022-04-0113580510.3390/genes13050805Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF BlastocystsSha Yang0Yuze Yang1Haisheng Hao2Weihua Du3Yunwei Pang4Shanjiang Zhao5Huiying Zou6Huabin Zhu7Peipei Zhang8Xueming Zhao9Institute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaBeijing General Station of Animal Husbandry, Beijing 100101, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaInstitute of Animal Sciences (IAS), Chinese Academy of Agricultural Sciences (CAAS), Beijing 100193, ChinaThe quality and developmental capacity of oocytes derived from in vitro maturation (IVM) remain unsatisfactory, which greatly impairs the efficiency and application of embryo technologies. The present experiment was designed to investigate the effect of the supplementation of EGF, IGF-1, and Cx37 in an IVM medium on the maturation quality and development ability of bovine oocytes. The cytoplasmic maturation events of oocytes and the quality of in vitro fertilization (IVF) blastocysts were examined to investigate the relative mechanisms. Our results showed that the nuclear maturation and blastocyst development after the IVF of oocytes treated with 25 μg/mL Cx37 or the combination of 50 ng/mL EGF and 100 ng/mL IGF-1 were significantly increased compared to those of the control group (<i>p</i> < 0.05). Furthermore, the blastocyst rate, and blastocyst total cell number and survival rate after vitrification of the EGF+IGF-1+Cx37 group, were significantly higher than those of the control group (<i>p</i> < 0.05), but lower than those of the FSH+LH+EGF+IGF-1+Cx37 group (<i>p</i> < 0.05). The transzonal projection (TZP) intensity, glutathione (GSH) level, and mitochondrial function of the EGF+IGF-1+Cx37 group were significantly higher than that of the control group, and lower than those of the FSH+LH+EGF+IGF-1+Cx37 group, in contrast to the results of the reactive oxygen species (ROS) levels. In conclusion, our results showed that the supplementation of 50 ng/mL EGF, 100 ng/mL IGF-1, and 25 μg/mL Cx37 in the IVM of bovine oocytes significantly improved their quality and developmental ability by increasing the TZP, mitochondrial function, and GSH level.https://www.mdpi.com/2073-4425/13/5/805IVMEGFIGFconnexin 37vitrificationbovine |
| spellingShingle | Sha Yang Yuze Yang Haisheng Hao Weihua Du Yunwei Pang Shanjiang Zhao Huiying Zou Huabin Zhu Peipei Zhang Xueming Zhao Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts Genes IVM EGF IGF connexin 37 vitrification bovine |
| title | Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts |
| title_full | Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts |
| title_fullStr | Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts |
| title_full_unstemmed | Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts |
| title_short | Supplementation of EGF, IGF-1, and Connexin 37 in IVM Medium Significantly Improved the Maturation of Bovine Oocytes and Vitrification of Their IVF Blastocysts |
| title_sort | supplementation of egf igf 1 and connexin 37 in ivm medium significantly improved the maturation of bovine oocytes and vitrification of their ivf blastocysts |
| topic | IVM EGF IGF connexin 37 vitrification bovine |
| url | https://www.mdpi.com/2073-4425/13/5/805 |
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