Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo Study
Hepatitis B virus (HBV) infection remains a major global health problem. It is therefore imperative to develop drugs for anti-hepatitis B with high-efficiency and low toxicity. Attracted by the observations and evidence that the symptoms of some patients from the Southern Fujian, China, suffering fr...
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| Format: | Article |
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MDPI AG
2021-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/4/195 |
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| author | Fei Tang Guanghua Huang Liping Lin Hong Yin Lili Shao Ruian Xu Xiuling Cui |
| author_facet | Fei Tang Guanghua Huang Liping Lin Hong Yin Lili Shao Ruian Xu Xiuling Cui |
| author_sort | Fei Tang |
| collection | DOAJ |
| description | Hepatitis B virus (HBV) infection remains a major global health problem. It is therefore imperative to develop drugs for anti-hepatitis B with high-efficiency and low toxicity. Attracted by the observations and evidence that the symptoms of some patients from the Southern Fujian, China, suffering from hepatitis B were alleviated after daily eating an edible marine mollusk, <i>Thais clavigera</i> (Küster 1860) (TCK). Water-soluble polysaccharide from TCK (TCKP1) was isolated and characterized. The anti-HBV activity of TCKP1 and its regulatory pathway were investigated on both HepG2.2.15 cell line and HBV transgenic mice. The data obtained from in vitro studies showed that TCKP1 significantly enhanced the production of IFN-α, and reduced the level of HBV antigens and HBV DNA in the supernatants of HepG2.2.15 cells in a dose-dependent manner with low cytotoxicity. The result of the study on the HBV transgenic mice further revealed that TCKP1 significantly decreased the level of transaminases, HBsAg, HBeAg, and HBV DNA in the serum, as well as HBsAg, HBeAg, HBV DNA, and HBV RNA in the liver of HBV transgenic (HBV-Tg) mice. Furthermore, TCKP1 exhibited equivalent inhibitory effect with the positive control tenofovir alafenamide (TAF) on the markers above except for HBV DNA even in low dosage in a mouse model. However, the TCKP1 high-dose group displayed stronger inhibition of transaminases and liver HBsAg, HBeAg, and HBV RNA when compared with those of TAF. Meanwhile, inflammation of the liver was, by pathological observation, relieved in a dose-dependent manner after being treated with TCKP1. In addition, elevated levels of interleukin-12 (IL-12) and interferon γ (IFN-γ), and reduced level of interleukin-4 (IL-4) in the serum were observed, indicating that the anti-HBV effect of TCKP1 was achieved by potentiating immunocyte function and regulating the balance of Th1/Th2 cytokines. |
| first_indexed | 2024-03-10T12:47:11Z |
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| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T12:47:11Z |
| publishDate | 2021-03-01 |
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| series | Marine Drugs |
| spelling | doaj.art-32c5c9219ca8443b87e35745b5f58c312023-11-21T13:21:42ZengMDPI AGMarine Drugs1660-33972021-03-0119419510.3390/md19040195Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo StudyFei Tang0Guanghua Huang1Liping Lin2Hong Yin3Lili Shao4Ruian Xu5Xiuling Cui6Engineering Research Center of Molecular Medicine, Ministry of Education, School of Medicine, Huaqiao University, Xiamen 361021, ChinaEngineering Research Center of Molecular Medicine, Ministry of Education, School of Medicine, Huaqiao University, Xiamen 361021, ChinaEngineering Research Center of Molecular Medicine, Ministry of Education, School of Medicine, Huaqiao University, Xiamen 361021, ChinaEngineering Research Center of Molecular Medicine, Ministry of Education, School of Medicine, Huaqiao University, Xiamen 361021, ChinaEngineering Research Center of Molecular Medicine, Ministry of Education, School of Medicine, Huaqiao University, Xiamen 361021, ChinaEngineering Research Center of Molecular Medicine, Ministry of Education, School of Medicine, Huaqiao University, Xiamen 361021, ChinaEngineering Research Center of Molecular Medicine, Ministry of Education, School of Medicine, Huaqiao University, Xiamen 361021, ChinaHepatitis B virus (HBV) infection remains a major global health problem. It is therefore imperative to develop drugs for anti-hepatitis B with high-efficiency and low toxicity. Attracted by the observations and evidence that the symptoms of some patients from the Southern Fujian, China, suffering from hepatitis B were alleviated after daily eating an edible marine mollusk, <i>Thais clavigera</i> (Küster 1860) (TCK). Water-soluble polysaccharide from TCK (TCKP1) was isolated and characterized. The anti-HBV activity of TCKP1 and its regulatory pathway were investigated on both HepG2.2.15 cell line and HBV transgenic mice. The data obtained from in vitro studies showed that TCKP1 significantly enhanced the production of IFN-α, and reduced the level of HBV antigens and HBV DNA in the supernatants of HepG2.2.15 cells in a dose-dependent manner with low cytotoxicity. The result of the study on the HBV transgenic mice further revealed that TCKP1 significantly decreased the level of transaminases, HBsAg, HBeAg, and HBV DNA in the serum, as well as HBsAg, HBeAg, HBV DNA, and HBV RNA in the liver of HBV transgenic (HBV-Tg) mice. Furthermore, TCKP1 exhibited equivalent inhibitory effect with the positive control tenofovir alafenamide (TAF) on the markers above except for HBV DNA even in low dosage in a mouse model. However, the TCKP1 high-dose group displayed stronger inhibition of transaminases and liver HBsAg, HBeAg, and HBV RNA when compared with those of TAF. Meanwhile, inflammation of the liver was, by pathological observation, relieved in a dose-dependent manner after being treated with TCKP1. In addition, elevated levels of interleukin-12 (IL-12) and interferon γ (IFN-γ), and reduced level of interleukin-4 (IL-4) in the serum were observed, indicating that the anti-HBV effect of TCKP1 was achieved by potentiating immunocyte function and regulating the balance of Th1/Th2 cytokines.https://www.mdpi.com/1660-3397/19/4/195<i>Thais clavigera</i> (Küster)polysaccharidesanti-HBV activitiesin vitroin vivo |
| spellingShingle | Fei Tang Guanghua Huang Liping Lin Hong Yin Lili Shao Ruian Xu Xiuling Cui Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo Study Marine Drugs <i>Thais clavigera</i> (Küster) polysaccharides anti-HBV activities in vitro in vivo |
| title | Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo Study |
| title_full | Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo Study |
| title_fullStr | Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo Study |
| title_full_unstemmed | Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo Study |
| title_short | Anti-HBV Activities of Polysaccharides from <i>Thais clavigera</i> (Küster) by In Vitro and In Vivo Study |
| title_sort | anti hbv activities of polysaccharides from i thais clavigera i kuster by in vitro and in vivo study |
| topic | <i>Thais clavigera</i> (Küster) polysaccharides anti-HBV activities in vitro in vivo |
| url | https://www.mdpi.com/1660-3397/19/4/195 |
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