Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode
Galactooligosaccharides obtained via β-galactosidase transgalactosylation have health-promoting properties and are widely recognized as effective prebiotics. Trehalose-based galactooligosaccharides could be introduced into food and pharmaceutical industries similarly to trehalose. In light of this,...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-07-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/8/1619 |
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| author | Martina Belkova Tatiana Janegova Eva Hrabarova Jozef Nahalka |
| author_facet | Martina Belkova Tatiana Janegova Eva Hrabarova Jozef Nahalka |
| author_sort | Martina Belkova |
| collection | DOAJ |
| description | Galactooligosaccharides obtained via β-galactosidase transgalactosylation have health-promoting properties and are widely recognized as effective prebiotics. Trehalose-based galactooligosaccharides could be introduced into food and pharmaceutical industries similarly to trehalose. In light of this, new technological approaches are needed. Recently, in vivo enzyme immobilizations for recombinant proteins have been introduced, and physiological aggregation into active inclusion bodies (aIBs) has emerged as one such method of in vivo immobilization. To prepare LacZ β-galactosidase in the form of aIBs, we used a short 10 amino acid aggregation-prone tag. These native protein particles were simply washed from the cell lysate and applied in trehalose galactosylation in a recycled batch mode. In this study, aIBs entrapped in alginate beads, encapsulated in alginate/cellulose sulfate/poly(methylene-co-guanidine) capsules and magnetized were compared with free aIBs. Alginate/cellulose sulfate/PMCG capsules showed more suitable properties and applicability for biotransformation of trehalose at its high concentration (25%, <i>w</i>/<i>v</i>) and elevated temperature (50 °C). |
| first_indexed | 2024-03-10T23:48:15Z |
| format | Article |
| id | doaj.art-32c8a84a9c7c4430a7fafed22c65de38 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T23:48:15Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-32c8a84a9c7c4430a7fafed22c65de382023-11-19T01:53:06ZengMDPI AGLife2075-17292023-07-01138161910.3390/life13081619Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch ModeMartina Belkova0Tatiana Janegova1Eva Hrabarova2Jozef Nahalka3Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dubravska Cesta 9, SK-84538 Bratislava, SlovakiaInstitute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dubravska Cesta 9, SK-84538 Bratislava, SlovakiaInstitute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dubravska Cesta 9, SK-84538 Bratislava, SlovakiaInstitute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dubravska Cesta 9, SK-84538 Bratislava, SlovakiaGalactooligosaccharides obtained via β-galactosidase transgalactosylation have health-promoting properties and are widely recognized as effective prebiotics. Trehalose-based galactooligosaccharides could be introduced into food and pharmaceutical industries similarly to trehalose. In light of this, new technological approaches are needed. Recently, in vivo enzyme immobilizations for recombinant proteins have been introduced, and physiological aggregation into active inclusion bodies (aIBs) has emerged as one such method of in vivo immobilization. To prepare LacZ β-galactosidase in the form of aIBs, we used a short 10 amino acid aggregation-prone tag. These native protein particles were simply washed from the cell lysate and applied in trehalose galactosylation in a recycled batch mode. In this study, aIBs entrapped in alginate beads, encapsulated in alginate/cellulose sulfate/poly(methylene-co-guanidine) capsules and magnetized were compared with free aIBs. Alginate/cellulose sulfate/PMCG capsules showed more suitable properties and applicability for biotransformation of trehalose at its high concentration (25%, <i>w</i>/<i>v</i>) and elevated temperature (50 °C).https://www.mdpi.com/2075-1729/13/8/1619β-galactosidasegalactooligosaccharidesLacZin vivo enzyme immobilizationactive inclusion bodiesalginate beads |
| spellingShingle | Martina Belkova Tatiana Janegova Eva Hrabarova Jozef Nahalka Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode Life β-galactosidase galactooligosaccharides LacZ in vivo enzyme immobilization active inclusion bodies alginate beads |
| title | Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode |
| title_full | Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode |
| title_fullStr | Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode |
| title_full_unstemmed | Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode |
| title_short | Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode |
| title_sort | physiologically aggregated lacz applied in trehalose galactosylation in a recycled batch mode |
| topic | β-galactosidase galactooligosaccharides LacZ in vivo enzyme immobilization active inclusion bodies alginate beads |
| url | https://www.mdpi.com/2075-1729/13/8/1619 |
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