The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice
Salt stress is a critical limiting factor for rice growth and production. Although numerous salt-tolerant genes have been identified, the mechanism underlying salt stress tolerance in rice remains unclear. This study reports the need for an uncharacterized WRKY transcription factor OsWRKY54 for rice...
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/19/11999 |
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| author | Jingjing Huang Fuhang Liu Dong Chao Boning Xin Kui Liu Shuling Cao Xingxiang Chen Liyun Peng Baolei Zhang Shan Fu Jixing Xia |
| author_facet | Jingjing Huang Fuhang Liu Dong Chao Boning Xin Kui Liu Shuling Cao Xingxiang Chen Liyun Peng Baolei Zhang Shan Fu Jixing Xia |
| author_sort | Jingjing Huang |
| collection | DOAJ |
| description | Salt stress is a critical limiting factor for rice growth and production. Although numerous salt-tolerant genes have been identified, the mechanism underlying salt stress tolerance in rice remains unclear. This study reports the need for an uncharacterized WRKY transcription factor OsWRKY54 for rice salt-tolerance. Salt stress resulted in a rapid induction of <i>OsWRKY54</i> expression in roots. Immunostaining analysis showed that it was mainly expressed in the stele. The loss of <i>OsWRKY54</i> resulted in greater Na accumulation in shoots and enhanced sensitivity of rice plants to salt stress. The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as <i>OsNHX4</i> and <i>OsHKT1;5.</i> Furthermore, OsWRKY54 was found to regulate <i>OsHKT1;5</i> expression by directly binding to the W-box motif in its promoter. Thus, these results indicated that <i>OsWRKY54</i> was a critical regulatory factor in salt tolerance in rice. |
| first_indexed | 2024-03-09T21:36:06Z |
| format | Article |
| id | doaj.art-32c8ccfc10154c46b84b64b99e66adea |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T21:36:06Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-32c8ccfc10154c46b84b64b99e66adea2023-11-23T20:42:55ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-10-0123191199910.3390/ijms231911999The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in RiceJingjing Huang0Fuhang Liu1Dong Chao2Boning Xin3Kui Liu4Shuling Cao5Xingxiang Chen6Liyun Peng7Baolei Zhang8Shan Fu9Jixing Xia10State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, ChinaSalt stress is a critical limiting factor for rice growth and production. Although numerous salt-tolerant genes have been identified, the mechanism underlying salt stress tolerance in rice remains unclear. This study reports the need for an uncharacterized WRKY transcription factor OsWRKY54 for rice salt-tolerance. Salt stress resulted in a rapid induction of <i>OsWRKY54</i> expression in roots. Immunostaining analysis showed that it was mainly expressed in the stele. The loss of <i>OsWRKY54</i> resulted in greater Na accumulation in shoots and enhanced sensitivity of rice plants to salt stress. The real-time quantitative PCR (qRT-PCR) and transcriptome analysis revealed that OsWRKY54 regulated the expression of some essential genes related to salt tolerance, such as <i>OsNHX4</i> and <i>OsHKT1;5.</i> Furthermore, OsWRKY54 was found to regulate <i>OsHKT1;5</i> expression by directly binding to the W-box motif in its promoter. Thus, these results indicated that <i>OsWRKY54</i> was a critical regulatory factor in salt tolerance in rice.https://www.mdpi.com/1422-0067/23/19/11999salinityriceWRKYtranscription factorRNA-seq |
| spellingShingle | Jingjing Huang Fuhang Liu Dong Chao Boning Xin Kui Liu Shuling Cao Xingxiang Chen Liyun Peng Baolei Zhang Shan Fu Jixing Xia The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice International Journal of Molecular Sciences salinity rice WRKY transcription factor RNA-seq |
| title | The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice |
| title_full | The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice |
| title_fullStr | The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice |
| title_full_unstemmed | The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice |
| title_short | The WRKY Transcription Factor OsWRKY54 Is Involved in Salt Tolerance in Rice |
| title_sort | wrky transcription factor oswrky54 is involved in salt tolerance in rice |
| topic | salinity rice WRKY transcription factor RNA-seq |
| url | https://www.mdpi.com/1422-0067/23/19/11999 |
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