Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population genetics
The white stork, Ciconia ciconia, is a model species for studies of bird migration and behavior, but previously published genetic markers are not informative enough to perform individual–based genetic studies. Following discovery using next generation sequencing, 11 polymorphic markers were selected...
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| Format: | Article |
| Language: | English |
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Museu de Ciències Naturals de Barcelona
2016-02-01
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| Series: | Animal Biodiversity and Conservation |
| Subjects: | |
| Online Access: | http://abc.museucienciesjournals.cat/files/ABC_39-1_pp_11-16.pdf |
| _version_ | 1811265255326613504 |
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| author | Feldman Turjeman, S. Centeno–Cuadros, A. Nathan, R. |
| author_facet | Feldman Turjeman, S. Centeno–Cuadros, A. Nathan, R. |
| author_sort | Feldman Turjeman, S. |
| collection | DOAJ |
| description | The white stork, Ciconia ciconia, is a model species for studies of bird migration and behavior, but previously published genetic markers are not informative enough to perform individual–based genetic studies. Following discovery using next generation sequencing, 11 polymorphic markers were selected and tested in samples from two study sites. The number of alleles per locus ranged from 2–10 with an average of 5.3. The mean observed and expected heterozygosities were 0.519 and 0.565 respectively. PID was adequately sensitive for population– and individual–based genetics studies. There was no significant evidence of allelic drop–out, null alleles, or other errors; one sample site deviated from Hardy–Weinberg equilibrium for two loci, but no loci deviated in both samples, suggesting utility of these markers. These markers can be used to answer a range of ecological questions including those related to genetic diversity, degree of natal philopatry, and genetic mating strategies. |
| first_indexed | 2024-04-12T20:19:05Z |
| format | Article |
| id | doaj.art-32cac6236d744d4f8ce23be173b3ccad |
| institution | Directory Open Access Journal |
| issn | 1578-665X |
| language | English |
| last_indexed | 2024-04-12T20:19:05Z |
| publishDate | 2016-02-01 |
| publisher | Museu de Ciències Naturals de Barcelona |
| record_format | Article |
| series | Animal Biodiversity and Conservation |
| spelling | doaj.art-32cac6236d744d4f8ce23be173b3ccad2022-12-22T03:18:02ZengMuseu de Ciències Naturals de BarcelonaAnimal Biodiversity and Conservation1578-665X2016-02-013911116Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population geneticsFeldman Turjeman, S.0Centeno–Cuadros, A.1Nathan, R. 2Hebrew Univ., Jerusalem, IsraelHebrew Univ., Jerusalem, IsraelHebrew Univ., Jerusalem, IsraelThe white stork, Ciconia ciconia, is a model species for studies of bird migration and behavior, but previously published genetic markers are not informative enough to perform individual–based genetic studies. Following discovery using next generation sequencing, 11 polymorphic markers were selected and tested in samples from two study sites. The number of alleles per locus ranged from 2–10 with an average of 5.3. The mean observed and expected heterozygosities were 0.519 and 0.565 respectively. PID was adequately sensitive for population– and individual–based genetics studies. There was no significant evidence of allelic drop–out, null alleles, or other errors; one sample site deviated from Hardy–Weinberg equilibrium for two loci, but no loci deviated in both samples, suggesting utility of these markers. These markers can be used to answer a range of ecological questions including those related to genetic diversity, degree of natal philopatry, and genetic mating strategies.http://abc.museucienciesjournals.cat/files/ABC_39-1_pp_11-16.pdfGenetic markersShort tandem repeatsRelatednessProbability of identityPolymorphismGenetic diversity |
| spellingShingle | Feldman Turjeman, S. Centeno–Cuadros, A. Nathan, R. Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population genetics Animal Biodiversity and Conservation Genetic markers Short tandem repeats Relatedness Probability of identity Polymorphism Genetic diversity |
| title | Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population genetics |
| title_full | Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population genetics |
| title_fullStr | Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population genetics |
| title_full_unstemmed | Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population genetics |
| title_short | Isolation and characterization of novel polymorphic microsatellite markers for the white stork, Ciconia ciconia : applications in individual–based and population genetics |
| title_sort | isolation and characterization of novel polymorphic microsatellite markers for the white stork ciconia ciconia applications in individual based and population genetics |
| topic | Genetic markers Short tandem repeats Relatedness Probability of identity Polymorphism Genetic diversity |
| url | http://abc.museucienciesjournals.cat/files/ABC_39-1_pp_11-16.pdf |
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