The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and Virology
Bungowannah virus is a novel porcine pestivirus identified in a disease outbreak in Australia in 2003. The aim of this study was to determine the outcome of infection of the pregnant pig with this virus. Twenty-four pregnant pigs were infected at days 35, 55, 75 or 90 of gestation. Blood, tonsillar...
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| Format: | Article |
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MDPI AG
2020-06-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/12/6/691 |
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| author | Deborah S. Finlaison Peter D. Kirkland |
| author_facet | Deborah S. Finlaison Peter D. Kirkland |
| author_sort | Deborah S. Finlaison |
| collection | DOAJ |
| description | Bungowannah virus is a novel porcine pestivirus identified in a disease outbreak in Australia in 2003. The aim of this study was to determine the outcome of infection of the pregnant pig with this virus. Twenty-four pregnant pigs were infected at days 35, 55, 75 or 90 of gestation. Blood, tonsillar and rectal swabs were collected from each pig at birth and then weekly until euthanasia or death. Tissues were sampled at necropsy. Viral load was measured by real-time reverse-transcription polymerase chain reaction (qRT-PCR) and antibody levels in serum by peroxidase-linked immunoassay. Bungowannah virus was detected in the serum and excretions of all infected pigs at birth regardless of the stage of gestation at which infection occurred. Persistent infections occurred following infection prior to the development of foetal immunocompetence. Unexpectedly some animals infected at day 55 of gestation later cleared the virus and seroconverted. Viraemia and viral shedding resolved quickest following infection in late gestation. |
| first_indexed | 2024-03-10T18:51:08Z |
| format | Article |
| id | doaj.art-32cbab0263a841c99869e95a0d12532e |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T18:51:08Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-32cbab0263a841c99869e95a0d12532e2023-11-20T05:05:01ZengMDPI AGViruses1999-49152020-06-0112669110.3390/v12060691The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and VirologyDeborah S. Finlaison0Peter D. Kirkland1Virology Laboratory, Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW 2568, AustraliaVirology Laboratory, Elizabeth Macarthur Agricultural Institute, New South Wales Department of Primary Industries, Menangle, NSW 2568, AustraliaBungowannah virus is a novel porcine pestivirus identified in a disease outbreak in Australia in 2003. The aim of this study was to determine the outcome of infection of the pregnant pig with this virus. Twenty-four pregnant pigs were infected at days 35, 55, 75 or 90 of gestation. Blood, tonsillar and rectal swabs were collected from each pig at birth and then weekly until euthanasia or death. Tissues were sampled at necropsy. Viral load was measured by real-time reverse-transcription polymerase chain reaction (qRT-PCR) and antibody levels in serum by peroxidase-linked immunoassay. Bungowannah virus was detected in the serum and excretions of all infected pigs at birth regardless of the stage of gestation at which infection occurred. Persistent infections occurred following infection prior to the development of foetal immunocompetence. Unexpectedly some animals infected at day 55 of gestation later cleared the virus and seroconverted. Viraemia and viral shedding resolved quickest following infection in late gestation.https://www.mdpi.com/1999-4915/12/6/691Bungowannah virusfoetuspestivirusporcinereal-time PCRserology |
| spellingShingle | Deborah S. Finlaison Peter D. Kirkland The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and Virology Viruses Bungowannah virus foetus pestivirus porcine real-time PCR serology |
| title | The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and Virology |
| title_full | The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and Virology |
| title_fullStr | The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and Virology |
| title_full_unstemmed | The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and Virology |
| title_short | The Outcome of Porcine Foetal Infection with Bungowannah Virus Is Dependent on the Stage of Gestation at Which Infection Occurs. Part 1: Serology and Virology |
| title_sort | outcome of porcine foetal infection with bungowannah virus is dependent on the stage of gestation at which infection occurs part 1 serology and virology |
| topic | Bungowannah virus foetus pestivirus porcine real-time PCR serology |
| url | https://www.mdpi.com/1999-4915/12/6/691 |
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