Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> Nymphs
In this study, we evaluated the effect of the climatic season and infection by <i>Trypanosoma cruzi</i>, etiological agent of Chagas disease, on the molting capacity of the triatomine vector <i>Mepraia spinolai</i> endemic to Chile. We used wild-caught first-to-fourth instar...
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MDPI AG
2023-03-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/3/272 |
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| author | Juan Botzotz Gabriel Méndez-Valdés Sylvia Ortiz Angélica López Carezza Botto-Mahan Aldo Solari |
| author_facet | Juan Botzotz Gabriel Méndez-Valdés Sylvia Ortiz Angélica López Carezza Botto-Mahan Aldo Solari |
| author_sort | Juan Botzotz |
| collection | DOAJ |
| description | In this study, we evaluated the effect of the climatic season and infection by <i>Trypanosoma cruzi</i>, etiological agent of Chagas disease, on the molting capacity of the triatomine vector <i>Mepraia spinolai</i> endemic to Chile. We used wild-caught first-to-fourth instar nymphs during cooling (fall and winter) and warming (spring) periods. After capturing, nymphs were fed at the laboratory, and maintained under optimal rearing conditions. Feeding was repeated 40 days later. We followed-up the molting events on 709 nymphs, recording one, two or the absence of molts after two feeding opportunities. Within the same climatic period, only infected second- and fourth-instar nymphs from the warming period showed a larger proportion of double molting compared to uninfected nymphs. Regarding the climatic period, infected and uninfected first- and fourth-instar nymphs exhibited a larger proportion of double molting in the warming and cooling periods, respectively. The pattern of non-molting nymph occurrence suggests they probably reach diapause by environmental stochasticity. The effect of the climatic period and <i>T. cruzi</i> infection on the development of <i>M. spinolai</i> is an instar-dependent phenomenon, highlighting the occurrence of finely synchronized processes at different moments of the life cycle of such an hemimetabolous insect as triatomines. |
| first_indexed | 2024-03-11T06:22:51Z |
| format | Article |
| id | doaj.art-32ccce2bbdf846ea8277fda3480a2dad |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T06:22:51Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-32ccce2bbdf846ea8277fda3480a2dad2023-11-17T11:46:18ZengMDPI AGInsects2075-44502023-03-0114327210.3390/insects14030272Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> NymphsJuan Botzotz0Gabriel Méndez-Valdés1Sylvia Ortiz2Angélica López3Carezza Botto-Mahan4Aldo Solari5Programa de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380000, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380000, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380000, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380000, ChileDepartamento de Ciencias Ecológicas, Facultad de Ciencias, Universidad de Chile, Santiago 7800003, ChilePrograma de Biología Celular y Molecular, Instituto de Ciencias Biomédicas, Facultad de Medicina, Universidad de Chile, Santiago 8380000, ChileIn this study, we evaluated the effect of the climatic season and infection by <i>Trypanosoma cruzi</i>, etiological agent of Chagas disease, on the molting capacity of the triatomine vector <i>Mepraia spinolai</i> endemic to Chile. We used wild-caught first-to-fourth instar nymphs during cooling (fall and winter) and warming (spring) periods. After capturing, nymphs were fed at the laboratory, and maintained under optimal rearing conditions. Feeding was repeated 40 days later. We followed-up the molting events on 709 nymphs, recording one, two or the absence of molts after two feeding opportunities. Within the same climatic period, only infected second- and fourth-instar nymphs from the warming period showed a larger proportion of double molting compared to uninfected nymphs. Regarding the climatic period, infected and uninfected first- and fourth-instar nymphs exhibited a larger proportion of double molting in the warming and cooling periods, respectively. The pattern of non-molting nymph occurrence suggests they probably reach diapause by environmental stochasticity. The effect of the climatic period and <i>T. cruzi</i> infection on the development of <i>M. spinolai</i> is an instar-dependent phenomenon, highlighting the occurrence of finely synchronized processes at different moments of the life cycle of such an hemimetabolous insect as triatomines.https://www.mdpi.com/2075-4450/14/3/272<i>Mepraia spinolai</i><i>Trypanosoma cruzi</i>instar-dependent moltingsylvatic kissing bugChagas disease vectormolting efficiency |
| spellingShingle | Juan Botzotz Gabriel Méndez-Valdés Sylvia Ortiz Angélica López Carezza Botto-Mahan Aldo Solari Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> Nymphs Insects <i>Mepraia spinolai</i> <i>Trypanosoma cruzi</i> instar-dependent molting sylvatic kissing bug Chagas disease vector molting efficiency |
| title | Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> Nymphs |
| title_full | Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> Nymphs |
| title_fullStr | Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> Nymphs |
| title_full_unstemmed | Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> Nymphs |
| title_short | Natural <i>Trypanosoma cruzi</i> Infection and Climatic Season Influence the Developmental Capacity in Field-Caught <i>Mepraia spinolai</i> Nymphs |
| title_sort | natural i trypanosoma cruzi i infection and climatic season influence the developmental capacity in field caught i mepraia spinolai i nymphs |
| topic | <i>Mepraia spinolai</i> <i>Trypanosoma cruzi</i> instar-dependent molting sylvatic kissing bug Chagas disease vector molting efficiency |
| url | https://www.mdpi.com/2075-4450/14/3/272 |
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