Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production
The data presented in this article relates to the manuscript entitled ‘Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production’, published in the Journal Metabolic Engineering [1]. In the article here, we present...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-04-01
|
| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340920301116 |
| _version_ | 1818153318327779328 |
|---|---|
| author | James D. Budge Tanya J. Knight Jane Povey Joanne Roobol Ian R. Brown Gurdeep Singh Andrew Dean Sarah Turner Colin M. Jaques Robert J. Young Andrew J. Racher C Mark Smales |
| author_facet | James D. Budge Tanya J. Knight Jane Povey Joanne Roobol Ian R. Brown Gurdeep Singh Andrew Dean Sarah Turner Colin M. Jaques Robert J. Young Andrew J. Racher C Mark Smales |
| author_sort | James D. Budge |
| collection | DOAJ |
| description | The data presented in this article relates to the manuscript entitled ‘Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production’, published in the Journal Metabolic Engineering [1]. In the article here, we present data examining the overexpression of the lipid metabolism modifying genes SCD1 and SREBF1 in CHO cells by densitometry of western blots and by using mass spectrometry to investigate the impact on specific lipid species. We also present immunofluorescence data at the protein level upon SCD1 and SREBF1 overexpression. The growth profile data during batch culture of control CHO cells and CHO cells engineered to overexpress SCD1 and SREBF1 during batch culture are also reported. Finally, we report data on the yields of model secretory recombinant proteins produced from control, SCD1 or SREBF1 engineered cells using a transient expression systems. Keywords: Chinese hamster ovary (CHO) cells, Lipid metabolism engineering, Cell line engineering, Difficult to express protein, Recombinant protein, Endoplasmic reticulum, Transcription factor engineering |
| first_indexed | 2024-12-11T14:08:42Z |
| format | Article |
| id | doaj.art-32d14f1acc3b4dd5998f753bc5772db3 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-11T14:08:42Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-32d14f1acc3b4dd5998f753bc5772db32022-12-22T01:03:32ZengElsevierData in Brief2352-34092020-04-0129Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein productionJames D. Budge0Tanya J. Knight1Jane Povey2Joanne Roobol3Ian R. Brown4Gurdeep Singh5Andrew Dean6Sarah Turner7Colin M. Jaques8Robert J. Young9Andrew J. Racher10C Mark Smales11Industrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UKIndustrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UKIndustrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UKIndustrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UKIndustrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UKIndustrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UKLonza Biologics, 228 Bath Road, Slough, SL1 4DX, UKLonza Biologics, 228 Bath Road, Slough, SL1 4DX, UKLonza Biologics, 228 Bath Road, Slough, SL1 4DX, UKCell Engineering Group, Lonza Biologics, Granta Park, Cambridge, CB21 6GS, UKLonza Biologics, 228 Bath Road, Slough, SL1 4DX, UKIndustrial Biotechnology Centre, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK; Corresponding author.The data presented in this article relates to the manuscript entitled ‘Engineering of Chinese hamster ovary cell lipid metabolism results in an expanded ER and enhanced recombinant biotherapeutic protein production’, published in the Journal Metabolic Engineering [1]. In the article here, we present data examining the overexpression of the lipid metabolism modifying genes SCD1 and SREBF1 in CHO cells by densitometry of western blots and by using mass spectrometry to investigate the impact on specific lipid species. We also present immunofluorescence data at the protein level upon SCD1 and SREBF1 overexpression. The growth profile data during batch culture of control CHO cells and CHO cells engineered to overexpress SCD1 and SREBF1 during batch culture are also reported. Finally, we report data on the yields of model secretory recombinant proteins produced from control, SCD1 or SREBF1 engineered cells using a transient expression systems. Keywords: Chinese hamster ovary (CHO) cells, Lipid metabolism engineering, Cell line engineering, Difficult to express protein, Recombinant protein, Endoplasmic reticulum, Transcription factor engineeringhttp://www.sciencedirect.com/science/article/pii/S2352340920301116 |
| spellingShingle | James D. Budge Tanya J. Knight Jane Povey Joanne Roobol Ian R. Brown Gurdeep Singh Andrew Dean Sarah Turner Colin M. Jaques Robert J. Young Andrew J. Racher C Mark Smales Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production Data in Brief |
| title | Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production |
| title_full | Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production |
| title_fullStr | Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production |
| title_full_unstemmed | Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production |
| title_short | Data for engineering lipid metabolism of Chinese hamster ovary (CHO) cells for enhanced recombinant protein production |
| title_sort | data for engineering lipid metabolism of chinese hamster ovary cho cells for enhanced recombinant protein production |
| url | http://www.sciencedirect.com/science/article/pii/S2352340920301116 |
| work_keys_str_mv | AT jamesdbudge dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT tanyajknight dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT janepovey dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT joanneroobol dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT ianrbrown dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT gurdeepsingh dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT andrewdean dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT sarahturner dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT colinmjaques dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT robertjyoung dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT andrewjracher dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction AT cmarksmales dataforengineeringlipidmetabolismofchinesehamsterovarychocellsforenhancedrecombinantproteinproduction |