Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni
Campylobacteriosis produced by Campylobacter jejuni is the main cause of bacterial gastroenteritis worldwide and the binding of this pathogen to fucosylated glycoconjugates expressed on host cells is a determining factor for the infection. The aim of this work was to synthesize and characterize core...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Carbohydrate Polymer Technologies and Applications |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666893923001056 |
| _version_ | 1797383644544761856 |
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| author | Giovanna Sandoval Larios José Ángel Huerta Ocampo Jose Andrei Sarabia Sainz Alfonso García Galaz Ana María Guzmán-Partida Tomás Jesús Madera Santana Gabriela Ramos-Clamont Montfort |
| author_facet | Giovanna Sandoval Larios José Ángel Huerta Ocampo Jose Andrei Sarabia Sainz Alfonso García Galaz Ana María Guzmán-Partida Tomás Jesús Madera Santana Gabriela Ramos-Clamont Montfort |
| author_sort | Giovanna Sandoval Larios |
| collection | DOAJ |
| description | Campylobacteriosis produced by Campylobacter jejuni is the main cause of bacterial gastroenteritis worldwide and the binding of this pathogen to fucosylated glycoconjugates expressed on host cells is a determining factor for the infection. The aim of this work was to synthesize and characterize core-shell nanoparticles from fucoidan neoglycans as potential glycomimetic ligands for Campylobacter jejuni. Fucoidan oligosaccharides (OFuc) were obtained by mild hydrolysis of fucoidan. OFuc were separated by ultrafiltration, characterized by dynamic light scattering (DLS), infrared spectroscopy (FTIR), Fluorophore-assisted carbohydrate electrophoresis and lectin recognition. The OFuc1 were then conjugated with bovine serum albumin (BSA) by controlled glycation. The formation of fucosylated BSA (BSA-OFuc1) was confirmed by FTIR, increased surface charge, polyacrylamide gel electrophoresis, mass spectrometry and Ulex europaeus I lectin recognition. Afterwards core-shell BSA/BSA-OFuc1 nanoparticles were obtained by crosslinking. These nanoparticles presented hemispherical form with size and charge distribution of 312.1 ± 3.2 nm and -34.4 ± 0.3 mV respectively and were selectively recognized by C. jejuni by an ELISA-like lectin assay (ELLA). These results revel the potential of core-shell nanoparticles from fucoidan neoglycans as glycomimetic ligands for Campylobacter jejuni and open further opportunities to explore potential applications in the pharmaceutic industry. |
| first_indexed | 2024-03-08T21:24:03Z |
| format | Article |
| id | doaj.art-32d232c6e52d450b968cc3eb23862eb1 |
| institution | Directory Open Access Journal |
| issn | 2666-8939 |
| language | English |
| last_indexed | 2024-03-08T21:24:03Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Carbohydrate Polymer Technologies and Applications |
| spelling | doaj.art-32d232c6e52d450b968cc3eb23862eb12023-12-21T07:37:48ZengElsevierCarbohydrate Polymer Technologies and Applications2666-89392023-12-016100384Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuniGiovanna Sandoval Larios0José Ángel Huerta Ocampo1Jose Andrei Sarabia Sainz2Alfonso García Galaz3Ana María Guzmán-Partida4Tomás Jesús Madera Santana5Gabriela Ramos-Clamont Montfort6Centro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera Gustavo Enrique Astiazarán Rosas, No. 46 83304, Hermosillo, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera Gustavo Enrique Astiazarán Rosas, No. 46 83304, Hermosillo, Sonora, MexicoDepartamento de Investigación en Física, Universidad de Sonora, Hermosillo 83190, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera Gustavo Enrique Astiazarán Rosas, No. 46 83304, Hermosillo, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera Gustavo Enrique Astiazarán Rosas, No. 46 83304, Hermosillo, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Tecnología de Alimentos de Origen Vegetal, Carretera Gustavo Enrique Astiazarán Rosas, No. 46 83304, Hermosillo, Sonora, MexicoCentro de Investigación en Alimentación y Desarrollo, A.C. Coordinación de Ciencia de los Alimentos, Carretera Gustavo Enrique Astiazarán Rosas, No. 46 83304, Hermosillo, Sonora, Mexico; Corresponding author.Campylobacteriosis produced by Campylobacter jejuni is the main cause of bacterial gastroenteritis worldwide and the binding of this pathogen to fucosylated glycoconjugates expressed on host cells is a determining factor for the infection. The aim of this work was to synthesize and characterize core-shell nanoparticles from fucoidan neoglycans as potential glycomimetic ligands for Campylobacter jejuni. Fucoidan oligosaccharides (OFuc) were obtained by mild hydrolysis of fucoidan. OFuc were separated by ultrafiltration, characterized by dynamic light scattering (DLS), infrared spectroscopy (FTIR), Fluorophore-assisted carbohydrate electrophoresis and lectin recognition. The OFuc1 were then conjugated with bovine serum albumin (BSA) by controlled glycation. The formation of fucosylated BSA (BSA-OFuc1) was confirmed by FTIR, increased surface charge, polyacrylamide gel electrophoresis, mass spectrometry and Ulex europaeus I lectin recognition. Afterwards core-shell BSA/BSA-OFuc1 nanoparticles were obtained by crosslinking. These nanoparticles presented hemispherical form with size and charge distribution of 312.1 ± 3.2 nm and -34.4 ± 0.3 mV respectively and were selectively recognized by C. jejuni by an ELISA-like lectin assay (ELLA). These results revel the potential of core-shell nanoparticles from fucoidan neoglycans as glycomimetic ligands for Campylobacter jejuni and open further opportunities to explore potential applications in the pharmaceutic industry.http://www.sciencedirect.com/science/article/pii/S2666893923001056Maillard fucosylationBiorecognitionFucoidan neoglycansCampylobacter jejuniCore-shell nanoparticlesGlycomimetic ligands |
| spellingShingle | Giovanna Sandoval Larios José Ángel Huerta Ocampo Jose Andrei Sarabia Sainz Alfonso García Galaz Ana María Guzmán-Partida Tomás Jesús Madera Santana Gabriela Ramos-Clamont Montfort Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni Carbohydrate Polymer Technologies and Applications Maillard fucosylation Biorecognition Fucoidan neoglycans Campylobacter jejuni Core-shell nanoparticles Glycomimetic ligands |
| title | Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni |
| title_full | Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni |
| title_fullStr | Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni |
| title_full_unstemmed | Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni |
| title_short | Core-shell nanoparticles from fucoidan neoglycans: Synthesis, characterization with capability of glycomimetic ligands for Campylobacter jejuni |
| title_sort | core shell nanoparticles from fucoidan neoglycans synthesis characterization with capability of glycomimetic ligands for campylobacter jejuni |
| topic | Maillard fucosylation Biorecognition Fucoidan neoglycans Campylobacter jejuni Core-shell nanoparticles Glycomimetic ligands |
| url | http://www.sciencedirect.com/science/article/pii/S2666893923001056 |
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