Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice.
How pathogenic bacteria adapt and evolve in the complex and variable environment of the host remains a largely unresolved question. Here we have used whole genome sequencing of Salmonella enterica serovar Typhimurium LT2 populations serially passaged in mice to identify mutations that adapt bacteria...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3723669?pdf=render |
| _version_ | 1818597625200377856 |
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| author | Sanna Koskiniemi Henry S Gibbons Linus Sandegren Naeem Anwar Gary Ouellette Stacey Broomall Mark Karavis Paul McGregor Alvin Liem Ed Fochler Lauren McNew Carolyn Nicole Rosenzweig Mikael Rhen Evan W Skowronski Dan I Andersson |
| author_facet | Sanna Koskiniemi Henry S Gibbons Linus Sandegren Naeem Anwar Gary Ouellette Stacey Broomall Mark Karavis Paul McGregor Alvin Liem Ed Fochler Lauren McNew Carolyn Nicole Rosenzweig Mikael Rhen Evan W Skowronski Dan I Andersson |
| author_sort | Sanna Koskiniemi |
| collection | DOAJ |
| description | How pathogenic bacteria adapt and evolve in the complex and variable environment of the host remains a largely unresolved question. Here we have used whole genome sequencing of Salmonella enterica serovar Typhimurium LT2 populations serially passaged in mice to identify mutations that adapt bacteria to systemic growth in mice. We found unique pathoadaptive mutations in two global regulators, phoQ and stpA, which increase the competitive indexes of the bacteria 3- to 5-fold. Also, all mouse-adapted lineages had changed the orientation of the hin invertable element, resulting in production of a FliC type of flagellum. Competition experiments in mice with locked flagellum mutants showed that strains expressing the FliC type of flagellum had a 5-fold increase in competitive index as compared to those expressing FljB type flagellum. Combination of the flagellum cassette inversion with the stpA mutation increased competitive indexes up to 20-fold. These experiments show that Salmonella can rapidly adapt to a mouse environment by acquiring a few mutations of moderate individual effect that when combined confer substantial increases in growth. |
| first_indexed | 2024-12-16T11:50:47Z |
| format | Article |
| id | doaj.art-32d286fab7f649d5b340ff9c0ec1e094 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T11:50:47Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-32d286fab7f649d5b340ff9c0ec1e0942022-12-21T22:32:42ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e7014710.1371/journal.pone.0070147Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice.Sanna KoskiniemiHenry S GibbonsLinus SandegrenNaeem AnwarGary OuelletteStacey BroomallMark KaravisPaul McGregorAlvin LiemEd FochlerLauren McNewCarolyn Nicole RosenzweigMikael RhenEvan W SkowronskiDan I AnderssonHow pathogenic bacteria adapt and evolve in the complex and variable environment of the host remains a largely unresolved question. Here we have used whole genome sequencing of Salmonella enterica serovar Typhimurium LT2 populations serially passaged in mice to identify mutations that adapt bacteria to systemic growth in mice. We found unique pathoadaptive mutations in two global regulators, phoQ and stpA, which increase the competitive indexes of the bacteria 3- to 5-fold. Also, all mouse-adapted lineages had changed the orientation of the hin invertable element, resulting in production of a FliC type of flagellum. Competition experiments in mice with locked flagellum mutants showed that strains expressing the FliC type of flagellum had a 5-fold increase in competitive index as compared to those expressing FljB type flagellum. Combination of the flagellum cassette inversion with the stpA mutation increased competitive indexes up to 20-fold. These experiments show that Salmonella can rapidly adapt to a mouse environment by acquiring a few mutations of moderate individual effect that when combined confer substantial increases in growth.http://europepmc.org/articles/PMC3723669?pdf=render |
| spellingShingle | Sanna Koskiniemi Henry S Gibbons Linus Sandegren Naeem Anwar Gary Ouellette Stacey Broomall Mark Karavis Paul McGregor Alvin Liem Ed Fochler Lauren McNew Carolyn Nicole Rosenzweig Mikael Rhen Evan W Skowronski Dan I Andersson Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice. PLoS ONE |
| title | Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice. |
| title_full | Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice. |
| title_fullStr | Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice. |
| title_full_unstemmed | Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice. |
| title_short | Pathoadaptive mutations in Salmonella enterica isolated after serial passage in mice. |
| title_sort | pathoadaptive mutations in salmonella enterica isolated after serial passage in mice |
| url | http://europepmc.org/articles/PMC3723669?pdf=render |
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