Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens
This study examines the effect of dietary supplementation with <i>Lactobacillus acidophilus</i> (LA) on the cholesterol levels, immune response, and productive performance of laying hens. A total of 216, 40-week-old, commercial Hy-Line brown chicken layers were randomly assigned into fou...
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MDPI AG
2020-09-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/10/9/1588 |
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| author | Abdulaziz A. Alaqil Ahmed O. Abbas Hossam S. El-Beltagi Hanaa. K. Abd El-Atty Gamal M. K. Mehaisen Eman S. Moustafa |
| author_facet | Abdulaziz A. Alaqil Ahmed O. Abbas Hossam S. El-Beltagi Hanaa. K. Abd El-Atty Gamal M. K. Mehaisen Eman S. Moustafa |
| author_sort | Abdulaziz A. Alaqil |
| collection | DOAJ |
| description | This study examines the effect of dietary supplementation with <i>Lactobacillus acidophilus</i> (LA) on the cholesterol levels, immune response, and productive performance of laying hens. A total of 216, 40-week-old, commercial Hy-Line brown chicken layers were randomly assigned into four treatment groups (18 birds × three replicates per group) and fed diet supplemented with 0 (control), 1 × 10<sup>9</sup>, 21 × 10<sup>9</sup>, and 31 × 10<sup>9</sup> colony forming units (CFUs) of <i>Lactobacillus acidophilus</i> (LA) per kg of feed for six consecutive weeks. Results show that plasma triglycerides, low-density lipoprotein (LDL) and total cholesterols became lesser, while high-density lipoprotein (HDL) cholesterol became higher in LA-supplemented groups compared to the control. In addition, a significant reduction occurred in the liver and egg yolk cholesterol by LA supplementation. Moreover, the immunological parameters including antibody titer against sheep red blood cells (SRBCs), phytohemagglutinin (PHA)-wattle swelling test, and T- & B-lymphocyte proliferation were enhanced in laying hens supplemented with LA compared to the control hens. While the heterophil to lymphocyte (H/L) ratio decreased with LA supplementation, indicating low stress conditions in the treated hens. These positive effects for LA were further reflected on the productive performance of laying hens and improved egg production, egg weight, egg mass, and feed efficiency. Our findings indicate that LA probiotic could be recommended in laying hens’ diets for lowering egg yolk cholesterol with positive impacts on health and performance. |
| first_indexed | 2024-03-10T16:31:35Z |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T16:31:35Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-32d30c4450184c46851fea77949ae3b52023-11-20T12:45:06ZengMDPI AGAnimals2076-26152020-09-01109158810.3390/ani10091588Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying HensAbdulaziz A. Alaqil0Ahmed O. Abbas1Hossam S. El-Beltagi2Hanaa. K. Abd El-Atty3Gamal M. K. Mehaisen4Eman S. Moustafa5Department of Animal and Fish Production, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi ArabiaDepartment of Animal and Fish Production, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi ArabiaDepartment of Agricultural Biotechnology, College of Agricultural and Food Sciences, King Faisal University, P.O. Box 420, Al-Ahsa 31982, Saudi ArabiaDepartment of Poultry Breeding, Animal Production Research Institute, Agricultural Research Center, Dokki, Giza 12611, EgyptDepartment of Animal Production, Faculty of Agriculture, Cairo University, Gamma St., Giza 12613, EgyptDepartment of Animal Production, Faculty of Agriculture, Cairo University, Gamma St., Giza 12613, EgyptThis study examines the effect of dietary supplementation with <i>Lactobacillus acidophilus</i> (LA) on the cholesterol levels, immune response, and productive performance of laying hens. A total of 216, 40-week-old, commercial Hy-Line brown chicken layers were randomly assigned into four treatment groups (18 birds × three replicates per group) and fed diet supplemented with 0 (control), 1 × 10<sup>9</sup>, 21 × 10<sup>9</sup>, and 31 × 10<sup>9</sup> colony forming units (CFUs) of <i>Lactobacillus acidophilus</i> (LA) per kg of feed for six consecutive weeks. Results show that plasma triglycerides, low-density lipoprotein (LDL) and total cholesterols became lesser, while high-density lipoprotein (HDL) cholesterol became higher in LA-supplemented groups compared to the control. In addition, a significant reduction occurred in the liver and egg yolk cholesterol by LA supplementation. Moreover, the immunological parameters including antibody titer against sheep red blood cells (SRBCs), phytohemagglutinin (PHA)-wattle swelling test, and T- & B-lymphocyte proliferation were enhanced in laying hens supplemented with LA compared to the control hens. While the heterophil to lymphocyte (H/L) ratio decreased with LA supplementation, indicating low stress conditions in the treated hens. These positive effects for LA were further reflected on the productive performance of laying hens and improved egg production, egg weight, egg mass, and feed efficiency. Our findings indicate that LA probiotic could be recommended in laying hens’ diets for lowering egg yolk cholesterol with positive impacts on health and performance.https://www.mdpi.com/2076-2615/10/9/1588<i>Lactobacillus acidophilus</i>cholesterol contentsimmunological parameterslaying performancechickens |
| spellingShingle | Abdulaziz A. Alaqil Ahmed O. Abbas Hossam S. El-Beltagi Hanaa. K. Abd El-Atty Gamal M. K. Mehaisen Eman S. Moustafa Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens Animals <i>Lactobacillus acidophilus</i> cholesterol contents immunological parameters laying performance chickens |
| title | Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens |
| title_full | Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens |
| title_fullStr | Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens |
| title_full_unstemmed | Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens |
| title_short | Dietary Supplementation of Probiotic <i>Lactobacillus acidophilus</i> Modulates Cholesterol Levels, Immune Response, and Productive Performance of Laying Hens |
| title_sort | dietary supplementation of probiotic i lactobacillus acidophilus i modulates cholesterol levels immune response and productive performance of laying hens |
| topic | <i>Lactobacillus acidophilus</i> cholesterol contents immunological parameters laying performance chickens |
| url | https://www.mdpi.com/2076-2615/10/9/1588 |
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