Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i>
C-type lectins (CTLs) are a family of carbohydrate-binding proteins that mediate multiple biological events, including adhesion between cells, the turnover of serum glycoproteins, and innate immune system reactions to prospective invaders. Here, we describe the cDNA cloning of lectin from the bivalv...
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MDPI AG
2023-01-01
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| 丛编: | Marine Drugs |
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| 在线阅读: | https://www.mdpi.com/1660-3397/21/2/55 |
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| author | Tatyana O. Mizgina Sergey N. Baldaev Galina N. Likhatskaya Valentina I. Molchanova Maxim S. Kokoulin Alina P. Filshtein Eugene A. Rogozhin Irina V. Chikalovets Marina P. Isaeva Oleg V. Chernikov |
| author_facet | Tatyana O. Mizgina Sergey N. Baldaev Galina N. Likhatskaya Valentina I. Molchanova Maxim S. Kokoulin Alina P. Filshtein Eugene A. Rogozhin Irina V. Chikalovets Marina P. Isaeva Oleg V. Chernikov |
| author_sort | Tatyana O. Mizgina |
| collection | DOAJ |
| description | C-type lectins (CTLs) are a family of carbohydrate-binding proteins that mediate multiple biological events, including adhesion between cells, the turnover of serum glycoproteins, and innate immune system reactions to prospective invaders. Here, we describe the cDNA cloning of lectin from the bivalve <i>Glycymeris yessoensis</i> (GYL), which encodes 161 amino acids and the C-type carbohydrate recognition domain (CRD) with EPN and WND motifs. The deduced amino acid sequence showed similarity to other CTLs. GYL is a glycoprotein containing two N-glycosylation sites per subunit. N-glycans are made up of xylose, mannose, D-glucosamine, 3-O-methylated galactose, D-quinovoses, and 3-O-methylated 6-deoxy-D-glucose. The potential CRD tertiary structure of the GYL adopted CTL-typical long-form double-loop structure and included three disulfide bridges at the bases of the loops. Additionally, when confirming the GYL sequence, eight isoforms of this lectin were identified. This fact indicates the presence of a multigene family of GYL-like C-type lectins in the bivalve <i>G. yessoensis</i>. Using the glycan microarray approach, natural carbohydrate ligands were established, and the glycotope for GYL was reconstructed as “Galβ1–4GlcNAcβ obligatory containing an additional fragment”, like a sulfate group or a methyl group of fucose or N-acetylgalactosamine residues. |
| first_indexed | 2024-03-11T08:31:16Z |
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| id | doaj.art-32d45f84effb4072a94ce529f04f6069 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T08:31:16Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-32d45f84effb4072a94ce529f04f60692023-11-16T21:47:12ZengMDPI AGMarine Drugs1660-33972023-01-012125510.3390/md21020055Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i>Tatyana O. Mizgina0Sergey N. Baldaev1Galina N. Likhatskaya2Valentina I. Molchanova3Maxim S. Kokoulin4Alina P. Filshtein5Eugene A. Rogozhin6Irina V. Chikalovets7Marina P. Isaeva8Oleg V. Chernikov9G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow 117997, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaG.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch of Russian Academy of Sciences, Vladivostok 690022, RussiaC-type lectins (CTLs) are a family of carbohydrate-binding proteins that mediate multiple biological events, including adhesion between cells, the turnover of serum glycoproteins, and innate immune system reactions to prospective invaders. Here, we describe the cDNA cloning of lectin from the bivalve <i>Glycymeris yessoensis</i> (GYL), which encodes 161 amino acids and the C-type carbohydrate recognition domain (CRD) with EPN and WND motifs. The deduced amino acid sequence showed similarity to other CTLs. GYL is a glycoprotein containing two N-glycosylation sites per subunit. N-glycans are made up of xylose, mannose, D-glucosamine, 3-O-methylated galactose, D-quinovoses, and 3-O-methylated 6-deoxy-D-glucose. The potential CRD tertiary structure of the GYL adopted CTL-typical long-form double-loop structure and included three disulfide bridges at the bases of the loops. Additionally, when confirming the GYL sequence, eight isoforms of this lectin were identified. This fact indicates the presence of a multigene family of GYL-like C-type lectins in the bivalve <i>G. yessoensis</i>. Using the glycan microarray approach, natural carbohydrate ligands were established, and the glycotope for GYL was reconstructed as “Galβ1–4GlcNAcβ obligatory containing an additional fragment”, like a sulfate group or a methyl group of fucose or N-acetylgalactosamine residues.https://www.mdpi.com/1660-3397/21/2/55bivalve lectinshemolymphC-type lectinsglycosylationmultigenic family |
| spellingShingle | Tatyana O. Mizgina Sergey N. Baldaev Galina N. Likhatskaya Valentina I. Molchanova Maxim S. Kokoulin Alina P. Filshtein Eugene A. Rogozhin Irina V. Chikalovets Marina P. Isaeva Oleg V. Chernikov Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i> Marine Drugs bivalve lectins hemolymph C-type lectins glycosylation multigenic family |
| title | Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i> |
| title_full | Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i> |
| title_fullStr | Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i> |
| title_full_unstemmed | Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i> |
| title_short | Molecular Cloning and Characteristics of a Lectin from the Bivalve <i>Glycymeris yessoensis</i> |
| title_sort | molecular cloning and characteristics of a lectin from the bivalve i glycymeris yessoensis i |
| topic | bivalve lectins hemolymph C-type lectins glycosylation multigenic family |
| url | https://www.mdpi.com/1660-3397/21/2/55 |
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