An in vivo study to investigate an original intramedullary bone graft harvesting technology
Abstract Background Harvesting bone graft (BG) from the intramedullary canal to treat bone defects is largely conducted using the Reamer–Irrigator–Aspirator (RIA) system. The RIA system uses irrigation fluid during harvesting, which may result in washout of osteoinductive factors. Here, we propose a...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-09-01
|
| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-023-01328-8 |
| _version_ | 1827723821323386880 |
|---|---|
| author | Markus Laubach Agathe Bessot Jacqui McGovern Siamak Saifzadeh Jonathan Gospos Daniel N. Segina Philipp Kobbe Frank Hildebrand Marie-Luise Wille Nathalie Bock Dietmar W. Hutmacher |
| author_facet | Markus Laubach Agathe Bessot Jacqui McGovern Siamak Saifzadeh Jonathan Gospos Daniel N. Segina Philipp Kobbe Frank Hildebrand Marie-Luise Wille Nathalie Bock Dietmar W. Hutmacher |
| author_sort | Markus Laubach |
| collection | DOAJ |
| description | Abstract Background Harvesting bone graft (BG) from the intramedullary canal to treat bone defects is largely conducted using the Reamer–Irrigator–Aspirator (RIA) system. The RIA system uses irrigation fluid during harvesting, which may result in washout of osteoinductive factors. Here, we propose a new harvesting technology dedicated to improving BG collection without the potential washout effect of osteoinductive factors associated with irrigation fluid. This novel technology involves the conceptual approach of first aspirating the bone marrow (BM) with a novel aspirator prototype, followed by reaming with standard reamers and collecting the bone chips with the aspirator (reaming–aspiration method, R–A method). The aim of this study was to assess the harvesting efficacy and osteoinductive profile of the BG harvested with RIA 2 system (RIA 2 group) compared to the novel harvesting concept (aspirator + R–A method, ARA group). Methods Pre-planning computed tomography (CT) imaging was conducted on 16 sheep to determine the femoral isthmus canal diameter. In this non-recovery study, sheep were divided into two groups: RIA 2 group (n = 8) and ARA group (n = 8). We measured BG weight collected from left femur and determined femoral cortical bone volume reduction in postoperative CT imaging. Growth factor and inflammatory cytokine amounts of the BGs were quantified using enzyme-linked immunosorbent assay (ELISA) methods. Results The use of the stand-alone novel aspirator in BM collection, and in harvesting BG when the aspirator is used in conjunction with sequential reaming (R–A method) was proven feasible. ELISA results showed that the collected BG contained relevant amounts of growth factors and inflammatory cytokines in both the RIA 2 and the ARA group. Conclusions Here, we present the first results of an innovative concept for harvesting intramedullary BG. It is a prototype of a novel aspirator technology that enables the stepwise harvesting of first BM and subsequent bone chips from the intramedullary canal of long bones. Both the BG collected with the RIA 2 system and the aspirator prototype had the capacity to preserve the BG’s osteoinductive microenvironment. Future in vivo studies are required to confirm the bone regenerative capacity of BG harvested with the innovative harvesting technology. |
| first_indexed | 2024-03-10T22:06:09Z |
| format | Article |
| id | doaj.art-32d79bebaca34c9ebd2ab6c4c0ba3152 |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-03-10T22:06:09Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-32d79bebaca34c9ebd2ab6c4c0ba31522023-11-19T12:47:00ZengBMCEuropean Journal of Medical Research2047-783X2023-09-0128111610.1186/s40001-023-01328-8An in vivo study to investigate an original intramedullary bone graft harvesting technologyMarkus Laubach0Agathe Bessot1Jacqui McGovern2Siamak Saifzadeh3Jonathan Gospos4Daniel N. Segina5Philipp Kobbe6Frank Hildebrand7Marie-Luise Wille8Nathalie Bock9Dietmar W. Hutmacher10Australian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of TechnologyMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of TechnologyMax Planck Queensland Centre (MPQC) for the Materials Science of Extracellular Matrices, Queensland University of TechnologyAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of TechnologyCentre for Biomedical Technologies, School of Mechanical, Medical and Process Engineering, Queensland University of TechnologyDepartment of Orthopaedics, Holmes Regional Trauma CenterDepartment of Trauma and Reconstructive Surgery, BG Klinikum BergmannstrostDepartment of Orthopaedics, Trauma and Reconstructive Surgery, RWTH Aachen University HospitalAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of TechnologyAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of TechnologyAustralian Research Council (ARC) Training Centre for Multiscale 3D Imaging, Modelling, and Manufacturing (M3D Innovation), Queensland University of TechnologyAbstract Background Harvesting bone graft (BG) from the intramedullary canal to treat bone defects is largely conducted using the Reamer–Irrigator–Aspirator (RIA) system. The RIA system uses irrigation fluid during harvesting, which may result in washout of osteoinductive factors. Here, we propose a new harvesting technology dedicated to improving BG collection without the potential washout effect of osteoinductive factors associated with irrigation fluid. This novel technology involves the conceptual approach of first aspirating the bone marrow (BM) with a novel aspirator prototype, followed by reaming with standard reamers and collecting the bone chips with the aspirator (reaming–aspiration method, R–A method). The aim of this study was to assess the harvesting efficacy and osteoinductive profile of the BG harvested with RIA 2 system (RIA 2 group) compared to the novel harvesting concept (aspirator + R–A method, ARA group). Methods Pre-planning computed tomography (CT) imaging was conducted on 16 sheep to determine the femoral isthmus canal diameter. In this non-recovery study, sheep were divided into two groups: RIA 2 group (n = 8) and ARA group (n = 8). We measured BG weight collected from left femur and determined femoral cortical bone volume reduction in postoperative CT imaging. Growth factor and inflammatory cytokine amounts of the BGs were quantified using enzyme-linked immunosorbent assay (ELISA) methods. Results The use of the stand-alone novel aspirator in BM collection, and in harvesting BG when the aspirator is used in conjunction with sequential reaming (R–A method) was proven feasible. ELISA results showed that the collected BG contained relevant amounts of growth factors and inflammatory cytokines in both the RIA 2 and the ARA group. Conclusions Here, we present the first results of an innovative concept for harvesting intramedullary BG. It is a prototype of a novel aspirator technology that enables the stepwise harvesting of first BM and subsequent bone chips from the intramedullary canal of long bones. Both the BG collected with the RIA 2 system and the aspirator prototype had the capacity to preserve the BG’s osteoinductive microenvironment. Future in vivo studies are required to confirm the bone regenerative capacity of BG harvested with the innovative harvesting technology.https://doi.org/10.1186/s40001-023-01328-8Bone graftHarvestingGrowth factorsCytokines |
| spellingShingle | Markus Laubach Agathe Bessot Jacqui McGovern Siamak Saifzadeh Jonathan Gospos Daniel N. Segina Philipp Kobbe Frank Hildebrand Marie-Luise Wille Nathalie Bock Dietmar W. Hutmacher An in vivo study to investigate an original intramedullary bone graft harvesting technology European Journal of Medical Research Bone graft Harvesting Growth factors Cytokines |
| title | An in vivo study to investigate an original intramedullary bone graft harvesting technology |
| title_full | An in vivo study to investigate an original intramedullary bone graft harvesting technology |
| title_fullStr | An in vivo study to investigate an original intramedullary bone graft harvesting technology |
| title_full_unstemmed | An in vivo study to investigate an original intramedullary bone graft harvesting technology |
| title_short | An in vivo study to investigate an original intramedullary bone graft harvesting technology |
| title_sort | in vivo study to investigate an original intramedullary bone graft harvesting technology |
| topic | Bone graft Harvesting Growth factors Cytokines |
| url | https://doi.org/10.1186/s40001-023-01328-8 |
| work_keys_str_mv | AT markuslaubach aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT agathebessot aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT jacquimcgovern aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT siamaksaifzadeh aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT jonathangospos aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT danielnsegina aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT philippkobbe aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT frankhildebrand aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT marieluisewille aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT nathaliebock aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT dietmarwhutmacher aninvivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT markuslaubach invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT agathebessot invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT jacquimcgovern invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT siamaksaifzadeh invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT jonathangospos invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT danielnsegina invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT philippkobbe invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT frankhildebrand invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT marieluisewille invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT nathaliebock invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology AT dietmarwhutmacher invivostudytoinvestigateanoriginalintramedullarybonegraftharvestingtechnology |