Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide
This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be...
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| Format: | Article |
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Czech Academy of Agricultural Sciences
2012-06-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
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| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201202-0001_pseudo-embryogenic-structures-in-anther-and-isolated-microspore-cultures-in-vitro-a-cautionary-guide.php |
| _version_ | 1828008704499253248 |
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| author | Ugur BAL Mehran E. SHARIATPANAHI Antonio J. CASTRO Delphine EMERY Christophe CLÉMENT Maryam DEHESTANI-ARDAKANI Khadijeh MOZAFFARI Alisher TOURAEV |
| author_facet | Ugur BAL Mehran E. SHARIATPANAHI Antonio J. CASTRO Delphine EMERY Christophe CLÉMENT Maryam DEHESTANI-ARDAKANI Khadijeh MOZAFFARI Alisher TOURAEV |
| author_sort | Ugur BAL |
| collection | DOAJ |
| description | This review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be named as pseudo-embryogenic structures. The introduction of pseudo-embryogenic structures and their origins could be helpful to distinguish them from true microspore-derived structures. Prompted by certain environmental cues, somatic cells existing as a contamination in immature pollen (microspores) cultures can lead to the formation of 'pseudo-embryos' commonly known as embryoids. The pseudo-embryogenic structures may be classified in the following groups: (i) pseudo-star-like structures; pseudo-multicellular structures; (ii) pseudo-embryos with pseudo-suspensors; (iii) contaminating bacteria appearing as callus colonies; (iv) calli and embryos of somatic origin; (v) giant tetrad-like structures; (vi) anther wall cells. The exact origin of these structures is discussed in this paper, and some recommendations are proposed in order to avoid misinterpretation. |
| first_indexed | 2024-04-10T08:29:13Z |
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| id | doaj.art-32d833d44779471f9464240d213b74ab |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:13Z |
| publishDate | 2012-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-32d833d44779471f9464240d213b74ab2023-02-23T03:30:15ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252012-06-01482516010.17221/176/2011-CJGPBcjg-201202-0001Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guideUgur BAL0Mehran E. SHARIATPANAHI1Antonio J. CASTRO2Delphine EMERY3Christophe CLÉMENT4Maryam DEHESTANI-ARDAKANI5Khadijeh MOZAFFARI6Alisher TOURAEV7Max F. Perutz Laboratories, Vienna University, Vienna, AustriaMax F. Perutz Laboratories, Vienna University, Vienna, AustriaSexual Plant Reproduction Laboratory, Spanish Council for Scientific Research (CSIC), Granada, SpainUniversity of Reims, UFR Sciences Exactes et Naturelles, Lab of Plant Stress Defense and Reproduction, Reims Cedex, France$3University of Reims, UFR Sciences Exactes et Naturelles, Lab of Plant Stress Defense and Reproduction, Reims Cedex, France$3Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, IranDepartment of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran, Karaj, IranMax F. Perutz Laboratories, Vienna University, Vienna, AustriaThis review describes sources of structures of non-microspore origin observed in anther and microspore cultures. Various characteristics of these structures may cause a wrong diagnosis of these structures as embryos or cell/tissue clusters of microspore origin. Here we suggest such structures to be named as pseudo-embryogenic structures. The introduction of pseudo-embryogenic structures and their origins could be helpful to distinguish them from true microspore-derived structures. Prompted by certain environmental cues, somatic cells existing as a contamination in immature pollen (microspores) cultures can lead to the formation of 'pseudo-embryos' commonly known as embryoids. The pseudo-embryogenic structures may be classified in the following groups: (i) pseudo-star-like structures; pseudo-multicellular structures; (ii) pseudo-embryos with pseudo-suspensors; (iii) contaminating bacteria appearing as callus colonies; (iv) calli and embryos of somatic origin; (v) giant tetrad-like structures; (vi) anther wall cells. The exact origin of these structures is discussed in this paper, and some recommendations are proposed in order to avoid misinterpretation.https://cjgpb.agriculturejournals.cz/artkey/cjg-201202-0001_pseudo-embryogenic-structures-in-anther-and-isolated-microspore-cultures-in-vitro-a-cautionary-guide.phpanther culturemicrospore embryogenesispseudo-embryogenic structures |
| spellingShingle | Ugur BAL Mehran E. SHARIATPANAHI Antonio J. CASTRO Delphine EMERY Christophe CLÉMENT Maryam DEHESTANI-ARDAKANI Khadijeh MOZAFFARI Alisher TOURAEV Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide Czech Journal of Genetics and Plant Breeding anther culture microspore embryogenesis pseudo-embryogenic structures |
| title | Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide |
| title_full | Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide |
| title_fullStr | Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide |
| title_full_unstemmed | Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide |
| title_short | Pseudo-embryogenic structures in anther and isolated microspore cultures in vitro: a cautionary guide |
| title_sort | pseudo embryogenic structures in anther and isolated microspore cultures in vitro a cautionary guide |
| topic | anther culture microspore embryogenesis pseudo-embryogenic structures |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201202-0001_pseudo-embryogenic-structures-in-anther-and-isolated-microspore-cultures-in-vitro-a-cautionary-guide.php |
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