Rapid detection and differentiation of mycobacterial species using a multiplex PCR system
Introduction The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods The purpose of this study was to evaluate...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Medicina Tropical (SBMT)
2013-07-01
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| Series: | Revista da Sociedade Brasileira de Medicina Tropical |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000400447&lng=en&tlng=en |
| _version_ | 1818242870551773184 |
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| author | Andrea Santos Lima Rafael Silva Duarte Lilian Maria Lapa Montenegro Haiana Charifker Schindler |
| author_facet | Andrea Santos Lima Rafael Silva Duarte Lilian Maria Lapa Montenegro Haiana Charifker Schindler |
| author_sort | Andrea Santos Lima |
| collection | DOAJ |
| description | Introduction The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobacterial strains as an auxiliary tool in the differential diagnosis of tuberculosis and diseases caused by nontuberculous mycobacteria (NTM) Results Forty mycobacterial strains isolated from pulmonary and extrapulmonary origin specimens from 37 patients diagnosed with tuberculosis were processed. Using phenotypic and biochemical characteristics of the 40 mycobacteria isolated in LJ medium, 57.5% (n=23) were characterized as the Mycobacterium tuberculosis complex (MTBC) and 20% (n=8) as nontuberculous mycobacteria (NTM), with 22.5% (n=9) of the results being inconclusive. When the results of the phenotypic and biochemical tests in 30 strains of mycobacteria were compared with the results of the multiplex PCR, there was 100% concordance in the identification of the MTBC and NTM species, respectively. A total of 32.5% (n=13) of the samples in multiplex PCR exhibited a molecular pattern consistent with NTM, thus disagreeing with the final diagnosis from the attending physician. Conclusions Multiplex PCR can be used as a differential method for determining TB infections caused by NTM a valuable tool in reducing the time necessary to make clinical diagnoses and begin treatment. It is also useful for identifying species that were previously not identifiable using conventional biochemical and phenotypic techniques. |
| first_indexed | 2024-12-12T13:52:06Z |
| format | Article |
| id | doaj.art-32d91a0a807e403f9d6c0c748aa68b2c |
| institution | Directory Open Access Journal |
| issn | 1678-9849 |
| language | English |
| last_indexed | 2024-12-12T13:52:06Z |
| publishDate | 2013-07-01 |
| publisher | Sociedade Brasileira de Medicina Tropical (SBMT) |
| record_format | Article |
| series | Revista da Sociedade Brasileira de Medicina Tropical |
| spelling | doaj.art-32d91a0a807e403f9d6c0c748aa68b2c2022-12-22T00:22:32ZengSociedade Brasileira de Medicina Tropical (SBMT)Revista da Sociedade Brasileira de Medicina Tropical1678-98492013-07-0146444745210.1590/0037-8682-0097-2013S0037-86822013000400447Rapid detection and differentiation of mycobacterial species using a multiplex PCR systemAndrea Santos LimaRafael Silva DuarteLilian Maria Lapa MontenegroHaiana Charifker SchindlerIntroduction The early diagnosis of mycobacterial infections is a critical step for initiating treatment and curing the patient. Molecular analytical methods have led to considerable improvements in the speed and accuracy of mycobacteria detection. Methods The purpose of this study was to evaluate a multiplex polymerase chain reaction system using mycobacterial strains as an auxiliary tool in the differential diagnosis of tuberculosis and diseases caused by nontuberculous mycobacteria (NTM) Results Forty mycobacterial strains isolated from pulmonary and extrapulmonary origin specimens from 37 patients diagnosed with tuberculosis were processed. Using phenotypic and biochemical characteristics of the 40 mycobacteria isolated in LJ medium, 57.5% (n=23) were characterized as the Mycobacterium tuberculosis complex (MTBC) and 20% (n=8) as nontuberculous mycobacteria (NTM), with 22.5% (n=9) of the results being inconclusive. When the results of the phenotypic and biochemical tests in 30 strains of mycobacteria were compared with the results of the multiplex PCR, there was 100% concordance in the identification of the MTBC and NTM species, respectively. A total of 32.5% (n=13) of the samples in multiplex PCR exhibited a molecular pattern consistent with NTM, thus disagreeing with the final diagnosis from the attending physician. Conclusions Multiplex PCR can be used as a differential method for determining TB infections caused by NTM a valuable tool in reducing the time necessary to make clinical diagnoses and begin treatment. It is also useful for identifying species that were previously not identifiable using conventional biochemical and phenotypic techniques.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000400447&lng=en&tlng=enMycobacterium tuberculosis complexNontuberculous mycobacteriaDiagnosisPolymerase chain reactionMultiplex |
| spellingShingle | Andrea Santos Lima Rafael Silva Duarte Lilian Maria Lapa Montenegro Haiana Charifker Schindler Rapid detection and differentiation of mycobacterial species using a multiplex PCR system Revista da Sociedade Brasileira de Medicina Tropical Mycobacterium tuberculosis complex Nontuberculous mycobacteria Diagnosis Polymerase chain reaction Multiplex |
| title | Rapid detection and differentiation of mycobacterial species using a multiplex PCR system |
| title_full | Rapid detection and differentiation of mycobacterial species using a multiplex PCR system |
| title_fullStr | Rapid detection and differentiation of mycobacterial species using a multiplex PCR system |
| title_full_unstemmed | Rapid detection and differentiation of mycobacterial species using a multiplex PCR system |
| title_short | Rapid detection and differentiation of mycobacterial species using a multiplex PCR system |
| title_sort | rapid detection and differentiation of mycobacterial species using a multiplex pcr system |
| topic | Mycobacterium tuberculosis complex Nontuberculous mycobacteria Diagnosis Polymerase chain reaction Multiplex |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822013000400447&lng=en&tlng=en |
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