Polyploidy induction in Phlox paniculata L. under in vitro conditions
The objective of this work was to find an effective method of polyploidy induction using chemomutagens, colchicine and oryzalin, in diploid cultivar of Phlox paniculata ‘Fujiyama’ (syn. Mt. Fuji, Fuji). Ploidy level was determined by the flow cytometry method (FCM). Two methods of treating the expla...
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| Format: | Article |
| Language: | English |
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Mendel University Press
2010-01-01
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| Series: | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| Subjects: | |
| Online Access: | https://acta.mendelu.cz/58/1/0101/ |
| _version_ | 1818541229229473792 |
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| author | Pavel Matiska Hana Vejsadová |
| author_facet | Pavel Matiska Hana Vejsadová |
| author_sort | Pavel Matiska |
| collection | DOAJ |
| description | The objective of this work was to find an effective method of polyploidy induction using chemomutagens, colchicine and oryzalin, in diploid cultivar of Phlox paniculata ‘Fujiyama’ (syn. Mt. Fuji, Fuji). Ploidy level was determined by the flow cytometry method (FCM). Two methods of treating the explants (in vitro regenerated shoots) were tested; chemomutagen infiltration from nutrient media (“the infiltration method”) and dipping of the explants in a chemical mutagen solution (“the dip method”). The highest values of tetraploid (5%), mixoploid (1.67%) frequency and polyploidization efficiency (1.25) were found in explants treated with 0.2% colchicine for 24 h in the dip method. Concentrations of 10 µM oryzalin and 0.2% colchicine for 14 d were the most effective for obtaining tetraploids in the infiltration method. The results will be exploited to other P. paniculata cultivars for breeding of this species. |
| first_indexed | 2024-12-11T22:05:53Z |
| format | Article |
| id | doaj.art-32d95b437c6e49aea8be3a051945862f |
| institution | Directory Open Access Journal |
| issn | 1211-8516 2464-8310 |
| language | English |
| last_indexed | 2024-12-11T22:05:53Z |
| publishDate | 2010-01-01 |
| publisher | Mendel University Press |
| record_format | Article |
| series | Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
| spelling | doaj.art-32d95b437c6e49aea8be3a051945862f2022-12-22T00:48:57ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102010-01-0158110110610.11118/actaun201058010101Polyploidy induction in Phlox paniculata L. under in vitro conditionsPavel Matiska0Hana Vejsadová1Česká zemědělská univerzita v Praze, Fakulta agrobiologie, potravinových a přírodních zdrojů, Kamýcká 129, 165 21 Praha 6, Česká republikaVýzkumný ústav Silva Taroucy pro krajinu a okrasné zahradnictví, v.v.i., Květnové nám. 391, 252 43 Průhonice, Česká republikaThe objective of this work was to find an effective method of polyploidy induction using chemomutagens, colchicine and oryzalin, in diploid cultivar of Phlox paniculata ‘Fujiyama’ (syn. Mt. Fuji, Fuji). Ploidy level was determined by the flow cytometry method (FCM). Two methods of treating the explants (in vitro regenerated shoots) were tested; chemomutagen infiltration from nutrient media (“the infiltration method”) and dipping of the explants in a chemical mutagen solution (“the dip method”). The highest values of tetraploid (5%), mixoploid (1.67%) frequency and polyploidization efficiency (1.25) were found in explants treated with 0.2% colchicine for 24 h in the dip method. Concentrations of 10 µM oryzalin and 0.2% colchicine for 14 d were the most effective for obtaining tetraploids in the infiltration method. The results will be exploited to other P. paniculata cultivars for breeding of this species.https://acta.mendelu.cz/58/1/0101/Phlox<i>in vitro</i> culturecolchicineoryzalinpolyploidizationflow cytometry method (FCM) |
| spellingShingle | Pavel Matiska Hana Vejsadová Polyploidy induction in Phlox paniculata L. under in vitro conditions Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis Phlox <i>in vitro</i> culture colchicine oryzalin polyploidization flow cytometry method (FCM) |
| title | Polyploidy induction in Phlox paniculata L. under in vitro conditions |
| title_full | Polyploidy induction in Phlox paniculata L. under in vitro conditions |
| title_fullStr | Polyploidy induction in Phlox paniculata L. under in vitro conditions |
| title_full_unstemmed | Polyploidy induction in Phlox paniculata L. under in vitro conditions |
| title_short | Polyploidy induction in Phlox paniculata L. under in vitro conditions |
| title_sort | polyploidy induction in phlox paniculata l under in vitro conditions |
| topic | Phlox <i>in vitro</i> culture colchicine oryzalin polyploidization flow cytometry method (FCM) |
| url | https://acta.mendelu.cz/58/1/0101/ |
| work_keys_str_mv | AT pavelmatiska polyploidyinductioninphloxpaniculatalunderinvitroconditions AT hanavejsadova polyploidyinductioninphloxpaniculatalunderinvitroconditions |