Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot
Abstract Background Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic c...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04383-9 |
| _version_ | 1827723606809903104 |
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| author | Bo Wang Ya-Hui Wang Yuan-Jie Deng Quan-Hong Yao Ai-Sheng Xiong |
| author_facet | Bo Wang Ya-Hui Wang Yuan-Jie Deng Quan-Hong Yao Ai-Sheng Xiong |
| author_sort | Bo Wang |
| collection | DOAJ |
| description | Abstract Background Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear. Results The accumulation of betanin in leaves can significantly improve its antioxidant capacity and induce a decrease of chlorophyll content. Transcriptome and metabolomics analysis showed that 14.0% of genes and 33.1% of metabolites were significantly different, and metabolic pathways related to photosynthesis and tyrosine metabolism were markedly altered. Combined analysis showed that phenylpropane biosynthesis pathway significantly enriched the differentially expressed genes and significantly altered metabolites. Conclusions Results showed that the metabolic status was significantly altered between transgenic and non-transgenic carrots, especially the photosynthesis and tyrosine metabolism. The extra consumption of tyrosine and accumulation of betanin might be the leading causes. |
| first_indexed | 2024-03-10T22:03:09Z |
| format | Article |
| id | doaj.art-32db56de6c7047749d77ec6beeefa376 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-10T22:03:09Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-32db56de6c7047749d77ec6beeefa3762023-11-19T12:53:08ZengBMCBMC Plant Biology1471-22292023-08-0123111310.1186/s12870-023-04383-9Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrotBo Wang0Ya-Hui Wang1Yuan-Jie Deng2Quan-Hong Yao3Ai-Sheng Xiong4State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural UniversityState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural UniversityShanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural ScienceState Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, College of Horticulture, Nanjing Agricultural UniversityAbstract Background Betalain is a natural pigment with important nutritional value and broad application prospects. Previously, we produced betanin biosynthesis transgenic carrots via expressing optimized genes CYP76AD1S, cDOPA5GTS and DODA1S. Betanin can accumulate throughout the whole transgenic carrots. But the effects of betanin accumulation on the metabolism of transgenic plants and whether it produces unexpected effects are still unclear. Results The accumulation of betanin in leaves can significantly improve its antioxidant capacity and induce a decrease of chlorophyll content. Transcriptome and metabolomics analysis showed that 14.0% of genes and 33.1% of metabolites were significantly different, and metabolic pathways related to photosynthesis and tyrosine metabolism were markedly altered. Combined analysis showed that phenylpropane biosynthesis pathway significantly enriched the differentially expressed genes and significantly altered metabolites. Conclusions Results showed that the metabolic status was significantly altered between transgenic and non-transgenic carrots, especially the photosynthesis and tyrosine metabolism. The extra consumption of tyrosine and accumulation of betanin might be the leading causes.https://doi.org/10.1186/s12870-023-04383-9Genetically modified carrotBetaninUnintended effectsPhotosynthesisTranscriptomeMetabolomics |
| spellingShingle | Bo Wang Ya-Hui Wang Yuan-Jie Deng Quan-Hong Yao Ai-Sheng Xiong Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot BMC Plant Biology Genetically modified carrot Betanin Unintended effects Photosynthesis Transcriptome Metabolomics |
| title | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
| title_full | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
| title_fullStr | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
| title_full_unstemmed | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
| title_short | Effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
| title_sort | effect of betanin synthesis on photosynthesis and tyrosine metabolism in transgenic carrot |
| topic | Genetically modified carrot Betanin Unintended effects Photosynthesis Transcriptome Metabolomics |
| url | https://doi.org/10.1186/s12870-023-04383-9 |
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