Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's disease
The triple transgenic mouse (3xTgAD), harboring human APPSwe, PS1M146V and TauP301L genes, develops age-dependent forebrain intraneuronal Aβ and tau as well as extraneuronal plaques. We evaluated brainstem AD-like pathology using 6E10, AT8, and Alz50 antibodies and unbiased stereology in young and o...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2009-09-01
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| Series: | Neurobiology of Disease |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0969996109001399 |
| _version_ | 1818673349027430400 |
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| author | Cassia R. Overk Christy M. Kelley Elliott J. Mufson |
| author_facet | Cassia R. Overk Christy M. Kelley Elliott J. Mufson |
| author_sort | Cassia R. Overk |
| collection | DOAJ |
| description | The triple transgenic mouse (3xTgAD), harboring human APPSwe, PS1M146V and TauP301L genes, develops age-dependent forebrain intraneuronal Aβ and tau as well as extraneuronal plaques. We evaluated brainstem AD-like pathology using 6E10, AT8, and Alz50 antibodies and unbiased stereology in young and old 3xTgAD mice. Intraneuronal Aβ occurred in the tectum, periaqueductal gray, substantia nigra, red nucleus, tegmentum and mesencephalic V nucleus at all ages. Aβ-positive neuron numbers significantly decreased in the superior colliculus and substantia nigra while AT8-positive superior colliculus, red nucleus, principal sensory V, vestibular nuclei, and tegmental neurons significantly increased between 2 and 12 months. Alz50-positive neuron numbers increased only in the inferior colliculus between these ages. Dual labeling revealed a few Aβ- and tau-positive neurons. Plaques occurred only in the pons of female 3xTgAD mice starting at 9 months. 3xTgAD mice provide a platform to define in vivo mechanisms of Aβ and tau brainstem pathology. |
| first_indexed | 2024-12-17T07:54:22Z |
| format | Article |
| id | doaj.art-32dfba0fd40c4dcdaa09e2bc29e66e55 |
| institution | Directory Open Access Journal |
| issn | 1095-953X |
| language | English |
| last_indexed | 2024-12-17T07:54:22Z |
| publishDate | 2009-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neurobiology of Disease |
| spelling | doaj.art-32dfba0fd40c4dcdaa09e2bc29e66e552022-12-21T21:57:46ZengElsevierNeurobiology of Disease1095-953X2009-09-01353415425Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's diseaseCassia R. Overk0Christy M. Kelley1Elliott J. Mufson2Department of Neurological Sciences, Rush University Medical Center, 1735 W. Harrison Street, Suite 300, Chicago, IL 60612, USADepartment of Neurological Sciences, Rush University Medical Center, 1735 W. Harrison Street, Suite 300, Chicago, IL 60612, USACorresponding author. Fax: +1 312 563 3571.; Department of Neurological Sciences, Rush University Medical Center, 1735 W. Harrison Street, Suite 300, Chicago, IL 60612, USAThe triple transgenic mouse (3xTgAD), harboring human APPSwe, PS1M146V and TauP301L genes, develops age-dependent forebrain intraneuronal Aβ and tau as well as extraneuronal plaques. We evaluated brainstem AD-like pathology using 6E10, AT8, and Alz50 antibodies and unbiased stereology in young and old 3xTgAD mice. Intraneuronal Aβ occurred in the tectum, periaqueductal gray, substantia nigra, red nucleus, tegmentum and mesencephalic V nucleus at all ages. Aβ-positive neuron numbers significantly decreased in the superior colliculus and substantia nigra while AT8-positive superior colliculus, red nucleus, principal sensory V, vestibular nuclei, and tegmental neurons significantly increased between 2 and 12 months. Alz50-positive neuron numbers increased only in the inferior colliculus between these ages. Dual labeling revealed a few Aβ- and tau-positive neurons. Plaques occurred only in the pons of female 3xTgAD mice starting at 9 months. 3xTgAD mice provide a platform to define in vivo mechanisms of Aβ and tau brainstem pathology.http://www.sciencedirect.com/science/article/pii/S0969996109001399Alzheimer's diseaseAmyloidBrainstemPathologyStereologyTau |
| spellingShingle | Cassia R. Overk Christy M. Kelley Elliott J. Mufson Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's disease Neurobiology of Disease Alzheimer's disease Amyloid Brainstem Pathology Stereology Tau |
| title | Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's disease |
| title_full | Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's disease |
| title_fullStr | Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's disease |
| title_full_unstemmed | Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's disease |
| title_short | Brainstem Alzheimer's-like pathology in the triple transgenic mouse model of Alzheimer's disease |
| title_sort | brainstem alzheimer s like pathology in the triple transgenic mouse model of alzheimer s disease |
| topic | Alzheimer's disease Amyloid Brainstem Pathology Stereology Tau |
| url | http://www.sciencedirect.com/science/article/pii/S0969996109001399 |
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