In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models
The extracellular microenvironment regulates cell decisions through the accurate presentation at the cell surface of a complex array of biochemical and biophysical signals that are mediated by the structure and composition of the extracellular matrix (ECM). On the one hand, the cells actively remode...
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| Format: | Article |
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Frontiers Media S.A.
2023-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1197075/full |
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| author | F. Urciuolo F. Urciuolo F. Urciuolo G. Imparato P. A. Netti P. A. Netti P. A. Netti |
| author_facet | F. Urciuolo F. Urciuolo F. Urciuolo G. Imparato P. A. Netti P. A. Netti P. A. Netti |
| author_sort | F. Urciuolo |
| collection | DOAJ |
| description | The extracellular microenvironment regulates cell decisions through the accurate presentation at the cell surface of a complex array of biochemical and biophysical signals that are mediated by the structure and composition of the extracellular matrix (ECM). On the one hand, the cells actively remodel the ECM, which on the other hand affects cell functions. This cell–ECM dynamic reciprocity is central in regulating and controlling morphogenetic and histogenetic processes. Misregulation within the extracellular space can cause aberrant bidirectional interactions between cells and ECM, resulting in dysfunctional tissues and pathological states. Therefore, tissue engineering approaches, aiming at reproducing organs and tissues in vitro, should realistically recapitulate the native cell–microenvironment crosstalk that is central for the correct functionality of tissue-engineered constructs. In this review, we will describe the most updated bioengineering approaches to recapitulate the native cell microenvironment and reproduce functional tissues and organs in vitro. We have highlighted the limitations of the use of exogenous scaffolds in recapitulating the regulatory/instructive and signal repository role of the native cell microenvironment. By contrast, strategies to reproduce human tissues and organs by inducing cells to synthetize their own ECM acting as a provisional scaffold to control and guide further tissue development and maturation hold the potential to allow the engineering of fully functional histologically competent three-dimensional (3D) tissues. |
| first_indexed | 2024-03-13T03:13:45Z |
| format | Article |
| id | doaj.art-32e4773fd474451584db74571ea5580a |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-03-13T03:13:45Z |
| publishDate | 2023-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-32e4773fd474451584db74571ea5580a2023-06-26T05:19:24ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-06-011110.3389/fbioe.2023.11970751197075In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture modelsF. Urciuolo0F. Urciuolo1F. Urciuolo2G. Imparato3P. A. Netti4P. A. Netti5P. A. Netti6Interdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Naples, ItalyDepartment of Chemical Materials and Industrial Production (DICMAPI), University of Naples Federico II, Naples, ItalyCenter for Advanced Biomaterials for HealthCare@CRIB, Istituto Italiano di Tecnologia, Naples, ItalyCenter for Advanced Biomaterials for HealthCare@CRIB, Istituto Italiano di Tecnologia, Naples, ItalyInterdisciplinary Research Centre on Biomaterials (CRIB), University of Naples Federico II, Naples, ItalyDepartment of Chemical Materials and Industrial Production (DICMAPI), University of Naples Federico II, Naples, ItalyCenter for Advanced Biomaterials for HealthCare@CRIB, Istituto Italiano di Tecnologia, Naples, ItalyThe extracellular microenvironment regulates cell decisions through the accurate presentation at the cell surface of a complex array of biochemical and biophysical signals that are mediated by the structure and composition of the extracellular matrix (ECM). On the one hand, the cells actively remodel the ECM, which on the other hand affects cell functions. This cell–ECM dynamic reciprocity is central in regulating and controlling morphogenetic and histogenetic processes. Misregulation within the extracellular space can cause aberrant bidirectional interactions between cells and ECM, resulting in dysfunctional tissues and pathological states. Therefore, tissue engineering approaches, aiming at reproducing organs and tissues in vitro, should realistically recapitulate the native cell–microenvironment crosstalk that is central for the correct functionality of tissue-engineered constructs. In this review, we will describe the most updated bioengineering approaches to recapitulate the native cell microenvironment and reproduce functional tissues and organs in vitro. We have highlighted the limitations of the use of exogenous scaffolds in recapitulating the regulatory/instructive and signal repository role of the native cell microenvironment. By contrast, strategies to reproduce human tissues and organs by inducing cells to synthetize their own ECM acting as a provisional scaffold to control and guide further tissue development and maturation hold the potential to allow the engineering of fully functional histologically competent three-dimensional (3D) tissues.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1197075/full3D tissue modelsextracellular matrixmorphogeneisdynamic reciprocitycell–ECM interaction |
| spellingShingle | F. Urciuolo F. Urciuolo F. Urciuolo G. Imparato P. A. Netti P. A. Netti P. A. Netti In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models Frontiers in Bioengineering and Biotechnology 3D tissue models extracellular matrix morphogeneis dynamic reciprocity cell–ECM interaction |
| title | In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models |
| title_full | In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models |
| title_fullStr | In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models |
| title_full_unstemmed | In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models |
| title_short | In vitro strategies for mimicking dynamic cell–ECM reciprocity in 3D culture models |
| title_sort | in vitro strategies for mimicking dynamic cell ecm reciprocity in 3d culture models |
| topic | 3D tissue models extracellular matrix morphogeneis dynamic reciprocity cell–ECM interaction |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1197075/full |
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