Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF
Ovarian tissue transplantation methods using cooled and cryopreserved samples have been attractive options for fertility preservation in animal models and humans. The aim of this study was to evaluate the impact of previous exposure to cooling, cryopreservation, and VEGF on the overall efficiency of...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Bioscientifica
2021-10-01
|
| Series: | Reproduction and Fertility |
| Subjects: | |
| Online Access: | https://raf.bioscientifica.com/view/journals/raf/2/4/RAF-21-0008.xml |
| _version_ | 1818994548322336768 |
|---|---|
| author | Samara Silva Souza Francisco Leo Nascimento Aguiar Benner Geraldo Alves Kele Amaral Alves Fabiana Aparecida Santilli Brandão Danielle Cristina Calado Brito Ramon da Silva Raposo Melba Oliveira Gastal Ana Paula Ribeiro Rodrigues José Ricardo Figueiredo Dárcio Ítalo Alves Teixeira Eduardo Leite Gastal |
| author_facet | Samara Silva Souza Francisco Leo Nascimento Aguiar Benner Geraldo Alves Kele Amaral Alves Fabiana Aparecida Santilli Brandão Danielle Cristina Calado Brito Ramon da Silva Raposo Melba Oliveira Gastal Ana Paula Ribeiro Rodrigues José Ricardo Figueiredo Dárcio Ítalo Alves Teixeira Eduardo Leite Gastal |
| author_sort | Samara Silva Souza |
| collection | DOAJ |
| description | Ovarian tissue transplantation methods using cooled and cryopreserved samples have been attractive options for fertility preservation in animal models and humans. The aim of this study was to evaluate the impact of previous exposure to cooling, cryopreservation, and VEGF on the overall efficiency of equine ovarian tissue after heterotopic xenotransplantation in mice. The endpoints evaluated were follicular morphology and development, follicular and stromal cell densities, angiogenesis (i.e. the density of new and mature blood vessels), collagen types I and III fiber densities, and total fibrosis. Ovaries of adult mares were harvested after ovariectomy, and ovarian fragments were xenografted in the i.p. wall of BALB nude mice. Ten types of treatments involving different combinations of cooling, cryopreservation, xenografting procedures, and VEGF exposure were compared. The novel aspect of this study was the use of equine ovarian tissue xenotransplantation in mice, challenging the fragments with different combinations of treatments. The main findings were (i) cooling but not cryopreservation was effective in preserving the follicular morphology, (ii) a greater percentage of developing follicles but lower follicular and stromal cell densities were observed after ovarian tissue engraftment, (iii) exposure to VEGF increased new and mature vessels in cryopreserved-transplanted tissue, and (iv) an appropriate balance in the collagen types I and III fiber ratio in cooling-transplanted tissue was observed after exposure to VEGF. This study contributes to advancing knowledge in the preservation of ovarian tissue after cooling-cryopreservation and transplantation aiming to be applied to genetically superior/valuable horses, livestock, endangered animals, and, possibly, humans. |
| first_indexed | 2024-12-20T20:59:42Z |
| format | Article |
| id | doaj.art-32e90087d92f4e26a474eb5a01dec84b |
| institution | Directory Open Access Journal |
| issn | 2633-8386 |
| language | English |
| last_indexed | 2024-12-20T20:59:42Z |
| publishDate | 2021-10-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | Reproduction and Fertility |
| spelling | doaj.art-32e90087d92f4e26a474eb5a01dec84b2022-12-21T19:26:44ZengBioscientificaReproduction and Fertility2633-83862021-10-0124251266https://doi.org/10.1530/RAF-21-0008Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGFSamara Silva Souza0Francisco Leo Nascimento Aguiar1Benner Geraldo Alves2Kele Amaral Alves3Fabiana Aparecida Santilli Brandão4Danielle Cristina Calado Brito5Ramon da Silva Raposo6Melba Oliveira Gastal7Ana Paula Ribeiro Rodrigues8José Ricardo Figueiredo9Dárcio Ítalo Alves Teixeira10Eduardo Leite Gastal11Laboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilDepartment of Veterinary Medicine, Sousa Campus, Federal Institute of Education, Science and Technology of Paraíba, Sousa, Paraíba, BrazilLaboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilLaboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilLaboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilLaboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilNucleus of Experimental Biology, University of Fortaleza, Fortaleza, Ceara, BrazilAnimal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, Illinois, USALaboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilLaboratory of Manipulation of Oocytes and Preantral Follicles, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilLaboratory of Diagnostic Imaging Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceara, Fortaleza, Ceara, BrazilAnimal Science, School of Agricultural Sciences, Southern Illinois University, Carbondale, Illinois, USAOvarian tissue transplantation methods using cooled and cryopreserved samples have been attractive options for fertility preservation in animal models and humans. The aim of this study was to evaluate the impact of previous exposure to cooling, cryopreservation, and VEGF on the overall efficiency of equine ovarian tissue after heterotopic xenotransplantation in mice. The endpoints evaluated were follicular morphology and development, follicular and stromal cell densities, angiogenesis (i.e. the density of new and mature blood vessels), collagen types I and III fiber densities, and total fibrosis. Ovaries of adult mares were harvested after ovariectomy, and ovarian fragments were xenografted in the i.p. wall of BALB nude mice. Ten types of treatments involving different combinations of cooling, cryopreservation, xenografting procedures, and VEGF exposure were compared. The novel aspect of this study was the use of equine ovarian tissue xenotransplantation in mice, challenging the fragments with different combinations of treatments. The main findings were (i) cooling but not cryopreservation was effective in preserving the follicular morphology, (ii) a greater percentage of developing follicles but lower follicular and stromal cell densities were observed after ovarian tissue engraftment, (iii) exposure to VEGF increased new and mature vessels in cryopreserved-transplanted tissue, and (iv) an appropriate balance in the collagen types I and III fiber ratio in cooling-transplanted tissue was observed after exposure to VEGF. This study contributes to advancing knowledge in the preservation of ovarian tissue after cooling-cryopreservation and transplantation aiming to be applied to genetically superior/valuable horses, livestock, endangered animals, and, possibly, humans.https://raf.bioscientifica.com/view/journals/raf/2/4/RAF-21-0008.xmlangiogenesismare ovarypreantral folliclestissue transplantationxenograft |
| spellingShingle | Samara Silva Souza Francisco Leo Nascimento Aguiar Benner Geraldo Alves Kele Amaral Alves Fabiana Aparecida Santilli Brandão Danielle Cristina Calado Brito Ramon da Silva Raposo Melba Oliveira Gastal Ana Paula Ribeiro Rodrigues José Ricardo Figueiredo Dárcio Ítalo Alves Teixeira Eduardo Leite Gastal Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF Reproduction and Fertility angiogenesis mare ovary preantral follicles tissue transplantation xenograft |
| title | Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF |
| title_full | Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF |
| title_fullStr | Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF |
| title_full_unstemmed | Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF |
| title_short | Equine ovarian tissue xenografting: impacts of cooling, vitrification, and VEGF |
| title_sort | equine ovarian tissue xenografting impacts of cooling vitrification and vegf |
| topic | angiogenesis mare ovary preantral follicles tissue transplantation xenograft |
| url | https://raf.bioscientifica.com/view/journals/raf/2/4/RAF-21-0008.xml |
| work_keys_str_mv | AT samarasilvasouza equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT franciscoleonascimentoaguiar equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT bennergeraldoalves equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT keleamaralalves equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT fabianaaparecidasantillibrandao equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT daniellecristinacaladobrito equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT ramondasilvaraposo equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT melbaoliveiragastal equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT anapaularibeirorodrigues equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT josericardofigueiredo equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT darcioitaloalvesteixeira equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf AT eduardoleitegastal equineovariantissuexenograftingimpactsofcoolingvitrificationandvegf |