Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>)
Asia Minor bluegrass (<i>Polypogon fugax</i> Nees ex Steud.) is a problematic grass weed of winter crops in China, where some populations have become resistant to herbicides. Previously, we identified a <i>P. fugax</i> population QS exhibiting target-site-based resistance (TS...
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MDPI AG
2022-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/12/2998 |
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| author | Fengyan Zhou Ziwei Meng Wei Tang Yunjing Han Yong Zhang |
| author_facet | Fengyan Zhou Ziwei Meng Wei Tang Yunjing Han Yong Zhang |
| author_sort | Fengyan Zhou |
| collection | DOAJ |
| description | Asia Minor bluegrass (<i>Polypogon fugax</i> Nees ex Steud.) is a problematic grass weed of winter crops in China, where some populations have become resistant to herbicides. Previously, we identified a <i>P. fugax</i> population QS exhibiting target-site-based resistance (TSR) and non-target-site-based resistance (NTSR) to clodinafop-propargyl. This study aims to understand the metabolic resistance to clodinafop-propargyl between susceptible (XC) and resistant (QS) populations of <i>P. fugax</i> in the seedling and tillering stage, separately. Several differentially expressed candidate genes in the seedling and tillering stages were identified by RNA-Seq, including three P450 family genes, one glutathione S-transferase (GST) gene, and two ATP-binding cassette transporters. Additionally, we discovered a GST gene that was significantly differentially expressed in the resistant population during the seedling stage, as well as three peroxidase genes that were presumed to be related to NTSR metabolism. Three other peroxidase genes and one esterase were presumed to be related to NTSR metabolism during the tillering stage of the resistant population. Overexpression of the three randomly selected candidate genes can enhance herbicide-resistance in Arabidopsis transgenic plants. This study provided a novel insight into herbicide metabolism regulation genes during the different growth stages of resistant <i>P. fugax</i> population. |
| first_indexed | 2024-03-09T17:25:47Z |
| format | Article |
| id | doaj.art-32ec34d33a63453e84fde809c7e5b026 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T17:25:47Z |
| publishDate | 2022-11-01 |
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| series | Agronomy |
| spelling | doaj.art-32ec34d33a63453e84fde809c7e5b0262023-11-24T12:44:42ZengMDPI AGAgronomy2073-43952022-11-011212299810.3390/agronomy12122998Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>)Fengyan Zhou0Ziwei Meng1Wei Tang2Yunjing Han3Yong Zhang4Institute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230001, ChinaInstitute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230001, ChinaState Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 311400, ChinaInstitute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230001, ChinaInstitute of Plant Protection and Agro-Products Safety, Anhui Academy of Agricultural Sciences, Hefei 230001, ChinaAsia Minor bluegrass (<i>Polypogon fugax</i> Nees ex Steud.) is a problematic grass weed of winter crops in China, where some populations have become resistant to herbicides. Previously, we identified a <i>P. fugax</i> population QS exhibiting target-site-based resistance (TSR) and non-target-site-based resistance (NTSR) to clodinafop-propargyl. This study aims to understand the metabolic resistance to clodinafop-propargyl between susceptible (XC) and resistant (QS) populations of <i>P. fugax</i> in the seedling and tillering stage, separately. Several differentially expressed candidate genes in the seedling and tillering stages were identified by RNA-Seq, including three P450 family genes, one glutathione S-transferase (GST) gene, and two ATP-binding cassette transporters. Additionally, we discovered a GST gene that was significantly differentially expressed in the resistant population during the seedling stage, as well as three peroxidase genes that were presumed to be related to NTSR metabolism. Three other peroxidase genes and one esterase were presumed to be related to NTSR metabolism during the tillering stage of the resistant population. Overexpression of the three randomly selected candidate genes can enhance herbicide-resistance in Arabidopsis transgenic plants. This study provided a novel insight into herbicide metabolism regulation genes during the different growth stages of resistant <i>P. fugax</i> population.https://www.mdpi.com/2073-4395/12/12/2998<i>Polypogon fugax</i>ACCase herbicide resistantnon-target-site-based resistance (NTSR)transcriptome analysisseedling stagetillering stage |
| spellingShingle | Fengyan Zhou Ziwei Meng Wei Tang Yunjing Han Yong Zhang Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>) Agronomy <i>Polypogon fugax</i> ACCase herbicide resistant non-target-site-based resistance (NTSR) transcriptome analysis seedling stage tillering stage |
| title | Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>) |
| title_full | Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>) |
| title_fullStr | Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>) |
| title_full_unstemmed | Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>) |
| title_short | Transcriptome Profiling to Identify Genes Involved in Non-Target-Site-Based Resistance to Clodinafop-Propargyl in Asia Minor Bluegrass (<i>Polypogon fugax</i>) |
| title_sort | transcriptome profiling to identify genes involved in non target site based resistance to clodinafop propargyl in asia minor bluegrass i polypogon fugax i |
| topic | <i>Polypogon fugax</i> ACCase herbicide resistant non-target-site-based resistance (NTSR) transcriptome analysis seedling stage tillering stage |
| url | https://www.mdpi.com/2073-4395/12/12/2998 |
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