UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels
Peripheral nerves are basic communication structures guiding motor and sensory information from the central nervous system to receptor units. Severed peripheral nerve injuries represent a large clinical problem with relevant challenges to successful synthetic nerve repair scaffolds as substitutes to...
| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/12/4/971 |
| _version_ | 1797570005219409920 |
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| author | Ruth Diez-Ahedo Xabier Mendibil Mari Carmen Márquez-Posadas Iban Quintana Francisco González Francisco Javier Rodríguez Leyla Zilic Colin Sherborne Adam Glen Caroline S. Taylor Frederik Claeyssens John W. Haycock Wandert Schaafsma Eva González Begoña Castro Santos Merino |
| author_facet | Ruth Diez-Ahedo Xabier Mendibil Mari Carmen Márquez-Posadas Iban Quintana Francisco González Francisco Javier Rodríguez Leyla Zilic Colin Sherborne Adam Glen Caroline S. Taylor Frederik Claeyssens John W. Haycock Wandert Schaafsma Eva González Begoña Castro Santos Merino |
| author_sort | Ruth Diez-Ahedo |
| collection | DOAJ |
| description | Peripheral nerves are basic communication structures guiding motor and sensory information from the central nervous system to receptor units. Severed peripheral nerve injuries represent a large clinical problem with relevant challenges to successful synthetic nerve repair scaffolds as substitutes to autologous nerve grafting. Numerous studies reported the use of hollow tubes made of synthetic polymers sutured between severed nerve stumps to promote nerve regeneration while providing protection for external factors, such as scar tissue formation and inflammation. Few approaches have described the potential use of a lumen structure comprised of microchannels or microfibers to provide axon growth avoiding misdirection and fostering proper healing. Here, we report the use of a 3D porous microchannel-based structure made of a photocurable methacrylated polycaprolactone, whose mechanical properties are comparable to native nerves. The neuro-regenerative properties of the polymer were assessed in vitro, prior to the implantation of the 3D porous structure, in a 6-mm rat sciatic nerve gap injury. The manufactured implants were biocompatible and able to be resorbed by the host’s body at a suitable rate, allowing the complete healing of the nerve. The innovative design of the highly porous structure with the axon guiding microchannels, along with the observation of myelinated axons and Schwann cells in the in vivo tests, led to a significant progress towards the standardized use of synthetic 3D multichannel-based structures in peripheral nerve surgery. |
| first_indexed | 2024-03-10T20:19:05Z |
| format | Article |
| id | doaj.art-32f1a51c078c4d68820d72a21b9d5906 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T20:19:05Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-32f1a51c078c4d68820d72a21b9d59062023-11-19T22:20:14ZengMDPI AGPolymers2073-43602020-04-0112497110.3390/polym12040971UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned MicrochannelsRuth Diez-Ahedo0Xabier Mendibil1Mari Carmen Márquez-Posadas2Iban Quintana3Francisco González4Francisco Javier Rodríguez5Leyla Zilic6Colin Sherborne7Adam Glen8Caroline S. Taylor9Frederik Claeyssens10John W. Haycock11Wandert Schaafsma12Eva González13Begoña Castro14Santos Merino15Tekniker, C/Iñaki Goenaga 5, 20600 Eibar, SpainTekniker, C/Iñaki Goenaga 5, 20600 Eibar, SpainTekniker, C/Iñaki Goenaga 5, 20600 Eibar, SpainTekniker, C/Iñaki Goenaga 5, 20600 Eibar, SpainLaboratory of Molecular Neurology, Hospital Nacional de Parapléjicos, Finca. la Peraleda s/n, 45071 Toledo, SpainLaboratory of Molecular Neurology, Hospital Nacional de Parapléjicos, Finca. la Peraleda s/n, 45071 Toledo, SpainDepartment of Materials Science & Engineering, University of Sheffield, Sheffield S3 7HQ, UKDepartment of Materials Science & Engineering, University of Sheffield, Sheffield S3 7HQ, UKDepartment of Materials Science & Engineering, University of Sheffield, Sheffield S3 7HQ, UKDepartment of Materials Science & Engineering, University of Sheffield, Sheffield S3 7HQ, UKDepartment of Materials Science & Engineering, University of Sheffield, Sheffield S3 7HQ, UKDepartment of Materials Science & Engineering, University of Sheffield, Sheffield S3 7HQ, UKHistocell S.L., Parque Tecnológico de Bizkaia, 801 A, 2, 48160 Derio, SpainHistocell S.L., Parque Tecnológico de Bizkaia, 801 A, 2, 48160 Derio, SpainHistocell S.L., Parque Tecnológico de Bizkaia, 801 A, 2, 48160 Derio, SpainTekniker, C/Iñaki Goenaga 5, 20600 Eibar, SpainPeripheral nerves are basic communication structures guiding motor and sensory information from the central nervous system to receptor units. Severed peripheral nerve injuries represent a large clinical problem with relevant challenges to successful synthetic nerve repair scaffolds as substitutes to autologous nerve grafting. Numerous studies reported the use of hollow tubes made of synthetic polymers sutured between severed nerve stumps to promote nerve regeneration while providing protection for external factors, such as scar tissue formation and inflammation. Few approaches have described the potential use of a lumen structure comprised of microchannels or microfibers to provide axon growth avoiding misdirection and fostering proper healing. Here, we report the use of a 3D porous microchannel-based structure made of a photocurable methacrylated polycaprolactone, whose mechanical properties are comparable to native nerves. The neuro-regenerative properties of the polymer were assessed in vitro, prior to the implantation of the 3D porous structure, in a 6-mm rat sciatic nerve gap injury. The manufactured implants were biocompatible and able to be resorbed by the host’s body at a suitable rate, allowing the complete healing of the nerve. The innovative design of the highly porous structure with the axon guiding microchannels, along with the observation of myelinated axons and Schwann cells in the in vivo tests, led to a significant progress towards the standardized use of synthetic 3D multichannel-based structures in peripheral nerve surgery.https://www.mdpi.com/2073-4360/12/4/971peripheral nervebiopolymerscaffoldmicrochannelsporositypolycaprolactone |
| spellingShingle | Ruth Diez-Ahedo Xabier Mendibil Mari Carmen Márquez-Posadas Iban Quintana Francisco González Francisco Javier Rodríguez Leyla Zilic Colin Sherborne Adam Glen Caroline S. Taylor Frederik Claeyssens John W. Haycock Wandert Schaafsma Eva González Begoña Castro Santos Merino UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels Polymers peripheral nerve biopolymer scaffold microchannels porosity polycaprolactone |
| title | UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels |
| title_full | UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels |
| title_fullStr | UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels |
| title_full_unstemmed | UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels |
| title_short | UV-Casting on Methacrylated PCL for the Production of a Peripheral Nerve Implant Containing an Array of Porous Aligned Microchannels |
| title_sort | uv casting on methacrylated pcl for the production of a peripheral nerve implant containing an array of porous aligned microchannels |
| topic | peripheral nerve biopolymer scaffold microchannels porosity polycaprolactone |
| url | https://www.mdpi.com/2073-4360/12/4/971 |
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