Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patients
Abstract Background Severe community-acquired pneumonia (S-CAP) is a public health threat, making it essential to identify novel biomarkers and investigate the underlying mechanisms of disease severity. Methods Here, we profiled host responses to S-CAP through proteomics analysis of plasma samples f...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
|
| Series: | Respiratory Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12931-024-02669-6 |
| _version_ | 1797349702078824448 |
|---|---|
| author | Jieqiong Li Yawen Wang Weichao Zhao Tingyu Yang Qianyu Zhang Huqin Yang Xuyan Li Zhaohui Tong |
| author_facet | Jieqiong Li Yawen Wang Weichao Zhao Tingyu Yang Qianyu Zhang Huqin Yang Xuyan Li Zhaohui Tong |
| author_sort | Jieqiong Li |
| collection | DOAJ |
| description | Abstract Background Severe community-acquired pneumonia (S-CAP) is a public health threat, making it essential to identify novel biomarkers and investigate the underlying mechanisms of disease severity. Methods Here, we profiled host responses to S-CAP through proteomics analysis of plasma samples from a cohort of S-CAP patients, non-severe (NS)-CAP patients, diseases controls (DCs), and healthy controls (HCs). Then, typical differentially expressed proteins were then validated by ELISA in an independent cohort. Metabolomics analysis was further performed on both the cohort 1 and cohort 2. Then, the proteomic and metabolomic signatures were compared between the adult and child cohorts to explore the characteristics of severe pneumonia patients. Results There were clear differences between CAP patients and controls, as well as substantial differences between the S-CAP and NS-CAP. Pathway analysis of changes revealed excessive inflammation, suppressed immunity, and lipid metabolic disorders in S-CAP cases. Interestingly, comparing these signatures between the adult and child cohorts confirmed that overactive inflammation and dysregulated lipid metabolism were common features of S-CAP patients, independent of age. The change proportion of glycerophospholipids, glycerolipids, and sphingolipids were obviously different in the adult and child S-CAP cases. Conclusion The plasma multi-omics profiling revealed that excessive inflammation, suppressed humoral immunity, and disordered metabolism are involved in S-CAP pathogenesis. |
| first_indexed | 2024-03-08T12:35:11Z |
| format | Article |
| id | doaj.art-32f3d2eb9e5548e695b58489e8e98bb7 |
| institution | Directory Open Access Journal |
| issn | 1465-993X |
| language | English |
| last_indexed | 2024-03-08T12:35:11Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Respiratory Research |
| spelling | doaj.art-32f3d2eb9e5548e695b58489e8e98bb72024-01-21T12:31:33ZengBMCRespiratory Research1465-993X2024-01-0125111510.1186/s12931-024-02669-6Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patientsJieqiong Li0Yawen Wang1Weichao Zhao2Tingyu Yang3Qianyu Zhang4Huqin Yang5Xuyan Li6Zhaohui Tong7Medical Research Center, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical UniversityDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical UniversityDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical UniversityMedical Research Center, Beijing Institute of Respiratory Medicine and Beijing Chao-Yang Hospital, Capital Medical UniversityDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical UniversityDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical UniversityDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical UniversityDepartment of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical UniversityAbstract Background Severe community-acquired pneumonia (S-CAP) is a public health threat, making it essential to identify novel biomarkers and investigate the underlying mechanisms of disease severity. Methods Here, we profiled host responses to S-CAP through proteomics analysis of plasma samples from a cohort of S-CAP patients, non-severe (NS)-CAP patients, diseases controls (DCs), and healthy controls (HCs). Then, typical differentially expressed proteins were then validated by ELISA in an independent cohort. Metabolomics analysis was further performed on both the cohort 1 and cohort 2. Then, the proteomic and metabolomic signatures were compared between the adult and child cohorts to explore the characteristics of severe pneumonia patients. Results There were clear differences between CAP patients and controls, as well as substantial differences between the S-CAP and NS-CAP. Pathway analysis of changes revealed excessive inflammation, suppressed immunity, and lipid metabolic disorders in S-CAP cases. Interestingly, comparing these signatures between the adult and child cohorts confirmed that overactive inflammation and dysregulated lipid metabolism were common features of S-CAP patients, independent of age. The change proportion of glycerophospholipids, glycerolipids, and sphingolipids were obviously different in the adult and child S-CAP cases. Conclusion The plasma multi-omics profiling revealed that excessive inflammation, suppressed humoral immunity, and disordered metabolism are involved in S-CAP pathogenesis.https://doi.org/10.1186/s12931-024-02669-6Severe community-acquired pneumoniaProteomicsMetabolomicsBiomarkerPathogenesis |
| spellingShingle | Jieqiong Li Yawen Wang Weichao Zhao Tingyu Yang Qianyu Zhang Huqin Yang Xuyan Li Zhaohui Tong Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patients Respiratory Research Severe community-acquired pneumonia Proteomics Metabolomics Biomarker Pathogenesis |
| title | Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patients |
| title_full | Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patients |
| title_fullStr | Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patients |
| title_full_unstemmed | Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patients |
| title_short | Multi-omics analysis reveals overactive inflammation and dysregulated metabolism in severe community-acquired pneumonia patients |
| title_sort | multi omics analysis reveals overactive inflammation and dysregulated metabolism in severe community acquired pneumonia patients |
| topic | Severe community-acquired pneumonia Proteomics Metabolomics Biomarker Pathogenesis |
| url | https://doi.org/10.1186/s12931-024-02669-6 |
| work_keys_str_mv | AT jieqiongli multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients AT yawenwang multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients AT weichaozhao multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients AT tingyuyang multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients AT qianyuzhang multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients AT huqinyang multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients AT xuyanli multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients AT zhaohuitong multiomicsanalysisrevealsoveractiveinflammationanddysregulatedmetabolisminseverecommunityacquiredpneumoniapatients |