Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture
Most Pb and Cd neurotoxicity studies investigate exposure to either of the toxic metals alone, while data on co-exposure are scarce. The aim of our study was to fill that gap by investigating acute combined effects of Pb and Cd on redox and essential metal status in the brain of Wistar rats. Animals...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Sciendo
2020-09-01
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| Series: | Arhiv za Higijenu Rada i Toksikologiju |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/aiht-2020-71-3425 |
| _version_ | 1818744766854070272 |
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| author | Javorac Dragana Đorđević Aleksandra Buha Anđelković Milena Tatović Simona Baralić Katarina Antonijević Evica Kotur-Stevuljević Jelena Đukić-Ćosić Danijela Antonijević Biljana Bulat Zorica |
| author_facet | Javorac Dragana Đorđević Aleksandra Buha Anđelković Milena Tatović Simona Baralić Katarina Antonijević Evica Kotur-Stevuljević Jelena Đukić-Ćosić Danijela Antonijević Biljana Bulat Zorica |
| author_sort | Javorac Dragana |
| collection | DOAJ |
| description | Most Pb and Cd neurotoxicity studies investigate exposure to either of the toxic metals alone, while data on co-exposure are scarce. The aim of our study was to fill that gap by investigating acute combined effects of Pb and Cd on redox and essential metal status in the brain of Wistar rats. Animals were randomised in four groups of six to eight rats, which received 15 or 30 mg/kg of Cd, 150 mg/kg of Pb, or 150 mg/kg of Pb + 15 mg/kg of Cd by gavage. The fifth, control, group received distilled water only. Co-treatment with Pb and Cd induced significant increase in malondialdehyde (MDA) and thiobarbituric acid-reactive substances (TBARS) compared to control and groups receiving either metal alone. This is of special importance, as MDA presence in the brain has been implicated in many neurodegenerative disorders. The groups did not significantly differ in Zn, Cu, Mn, and Fe brain levels. Our findings highlight the importance of metal mixture studies. Neurotoxicity assessments of single chemicals do not provide a real insight into exposure to mixtures in real life. Further research should look into interactions between these metals to reveal complex molecular mechanisms of their neurotoxicity. |
| first_indexed | 2024-12-18T02:49:32Z |
| format | Article |
| id | doaj.art-32f4e8a347b94781acce7cdae6a43c47 |
| institution | Directory Open Access Journal |
| issn | 1848-6312 |
| language | English |
| last_indexed | 2024-12-18T02:49:32Z |
| publishDate | 2020-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Arhiv za Higijenu Rada i Toksikologiju |
| spelling | doaj.art-32f4e8a347b94781acce7cdae6a43c472022-12-21T21:23:29ZengSciendoArhiv za Higijenu Rada i Toksikologiju1848-63122020-09-0171319720410.2478/aiht-2020-71-3425Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixtureJavorac Dragana0Đorđević Aleksandra Buha1Anđelković Milena2Tatović Simona3Baralić Katarina4Antonijević Evica5Kotur-Stevuljević Jelena6Đukić-Ćosić Danijela7Antonijević Biljana8Bulat Zorica9University of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Biochemistry, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaUniversity of Belgrade Faculty of Pharmacy, Department of Toxicology “Akademik Danilo Soldatović”, Belgrade, SerbiaMost Pb and Cd neurotoxicity studies investigate exposure to either of the toxic metals alone, while data on co-exposure are scarce. The aim of our study was to fill that gap by investigating acute combined effects of Pb and Cd on redox and essential metal status in the brain of Wistar rats. Animals were randomised in four groups of six to eight rats, which received 15 or 30 mg/kg of Cd, 150 mg/kg of Pb, or 150 mg/kg of Pb + 15 mg/kg of Cd by gavage. The fifth, control, group received distilled water only. Co-treatment with Pb and Cd induced significant increase in malondialdehyde (MDA) and thiobarbituric acid-reactive substances (TBARS) compared to control and groups receiving either metal alone. This is of special importance, as MDA presence in the brain has been implicated in many neurodegenerative disorders. The groups did not significantly differ in Zn, Cu, Mn, and Fe brain levels. Our findings highlight the importance of metal mixture studies. Neurotoxicity assessments of single chemicals do not provide a real insight into exposure to mixtures in real life. Further research should look into interactions between these metals to reveal complex molecular mechanisms of their neurotoxicity.https://doi.org/10.2478/aiht-2020-71-3425cdcufemdamnneurotoxicityoxidative stresspbtbarszncdcufemdamnneurotoksičnostoksidacijski strespbtbarszn |
| spellingShingle | Javorac Dragana Đorđević Aleksandra Buha Anđelković Milena Tatović Simona Baralić Katarina Antonijević Evica Kotur-Stevuljević Jelena Đukić-Ćosić Danijela Antonijević Biljana Bulat Zorica Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture Arhiv za Higijenu Rada i Toksikologiju cd cu fe mda mn neurotoxicity oxidative stress pb tbars zn cd cu fe mda mn neurotoksičnost oksidacijski stres pb tbars zn |
| title | Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture |
| title_full | Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture |
| title_fullStr | Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture |
| title_full_unstemmed | Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture |
| title_short | Redox and essential metal status in the brain of Wistar rats acutely exposed to a cadmium and lead mixture |
| title_sort | redox and essential metal status in the brain of wistar rats acutely exposed to a cadmium and lead mixture |
| topic | cd cu fe mda mn neurotoxicity oxidative stress pb tbars zn cd cu fe mda mn neurotoksičnost oksidacijski stres pb tbars zn |
| url | https://doi.org/10.2478/aiht-2020-71-3425 |
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