Engineering intelligent chassis cells via recombinase-based MEMORY circuits
Abstract Synthetic biologists seek to engineer intelligent living systems capable of decision-making, communication, and memory. Separate technologies exist for each tenet of intelligence; however, the unification of all three properties in a living system has not been achieved. Here, we engineer co...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
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Nature Portfolio
2024-03-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-024-46755-1 |
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author | Brian D. Huang Dowan Kim Yongjoon Yu Corey J. Wilson |
author_facet | Brian D. Huang Dowan Kim Yongjoon Yu Corey J. Wilson |
author_sort | Brian D. Huang |
collection | DOAJ |
description | Abstract Synthetic biologists seek to engineer intelligent living systems capable of decision-making, communication, and memory. Separate technologies exist for each tenet of intelligence; however, the unification of all three properties in a living system has not been achieved. Here, we engineer completely intelligent Escherichia coli strains that harbor six orthogonal and inducible genome-integrated recombinases, forming Molecularly Encoded Memory via an Orthogonal Recombinase arraY (MEMORY). MEMORY chassis cells facilitate intelligence via the discrete multi-input regulation of recombinase functions enabling inheritable DNA inversions, deletions, and genomic insertions. MEMORY cells can achieve programmable and permanent gain (or loss) of functions extrachromosomally or from a specific genomic locus, without the loss or modification of the MEMORY platform – enabling the sequential programming and reprogramming of DNA circuits within the cell. We demonstrate all three tenets of intelligence via a probiotic (Nissle 1917) MEMORY strain capable of information exchange with the gastrointestinal commensal Bacteroides thetaiotaomicron. |
first_indexed | 2024-04-24T19:54:42Z |
format | Article |
id | doaj.art-32f676996b1c4a7d9964cfa5c4d5e394 |
institution | Directory Open Access Journal |
issn | 2041-1723 |
language | English |
last_indexed | 2024-04-24T19:54:42Z |
publishDate | 2024-03-01 |
publisher | Nature Portfolio |
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series | Nature Communications |
spelling | doaj.art-32f676996b1c4a7d9964cfa5c4d5e3942024-03-24T12:25:26ZengNature PortfolioNature Communications2041-17232024-03-0115111710.1038/s41467-024-46755-1Engineering intelligent chassis cells via recombinase-based MEMORY circuitsBrian D. Huang0Dowan Kim1Yongjoon Yu2Corey J. Wilson3Georgia Institute of Technology, School of Chemical & Biomolecular EngineeringGeorgia Institute of Technology, School of Chemical & Biomolecular EngineeringGeorgia Institute of Technology, School of Chemical & Biomolecular EngineeringGeorgia Institute of Technology, School of Chemical & Biomolecular EngineeringAbstract Synthetic biologists seek to engineer intelligent living systems capable of decision-making, communication, and memory. Separate technologies exist for each tenet of intelligence; however, the unification of all three properties in a living system has not been achieved. Here, we engineer completely intelligent Escherichia coli strains that harbor six orthogonal and inducible genome-integrated recombinases, forming Molecularly Encoded Memory via an Orthogonal Recombinase arraY (MEMORY). MEMORY chassis cells facilitate intelligence via the discrete multi-input regulation of recombinase functions enabling inheritable DNA inversions, deletions, and genomic insertions. MEMORY cells can achieve programmable and permanent gain (or loss) of functions extrachromosomally or from a specific genomic locus, without the loss or modification of the MEMORY platform – enabling the sequential programming and reprogramming of DNA circuits within the cell. We demonstrate all three tenets of intelligence via a probiotic (Nissle 1917) MEMORY strain capable of information exchange with the gastrointestinal commensal Bacteroides thetaiotaomicron.https://doi.org/10.1038/s41467-024-46755-1 |
spellingShingle | Brian D. Huang Dowan Kim Yongjoon Yu Corey J. Wilson Engineering intelligent chassis cells via recombinase-based MEMORY circuits Nature Communications |
title | Engineering intelligent chassis cells via recombinase-based MEMORY circuits |
title_full | Engineering intelligent chassis cells via recombinase-based MEMORY circuits |
title_fullStr | Engineering intelligent chassis cells via recombinase-based MEMORY circuits |
title_full_unstemmed | Engineering intelligent chassis cells via recombinase-based MEMORY circuits |
title_short | Engineering intelligent chassis cells via recombinase-based MEMORY circuits |
title_sort | engineering intelligent chassis cells via recombinase based memory circuits |
url | https://doi.org/10.1038/s41467-024-46755-1 |
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