Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly
Larval diets used for artificial rearing can have a significant effect on insect biology. The Queensland fruit fly (aka “Qfly”), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), is one of the greatest challenges for fruit growers in Australia. The sterile insect technique (SIT) is being develope...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-09-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.576156/full |
| _version_ | 1818878670999126016 |
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| author | Rajib Majumder Rajib Majumder Brodie Sutcliffe Saleh Mohammad Adnan Saleh Mohammad Adnan Bishwo Mainali Bernard C. Dominiak Phillip W. Taylor Toni A. Chapman Toni A. Chapman |
| author_facet | Rajib Majumder Rajib Majumder Brodie Sutcliffe Saleh Mohammad Adnan Saleh Mohammad Adnan Bishwo Mainali Bernard C. Dominiak Phillip W. Taylor Toni A. Chapman Toni A. Chapman |
| author_sort | Rajib Majumder |
| collection | DOAJ |
| description | Larval diets used for artificial rearing can have a significant effect on insect biology. The Queensland fruit fly (aka “Qfly”), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), is one of the greatest challenges for fruit growers in Australia. The sterile insect technique (SIT) is being developed to manage outbreaks in regions that remain free of Qfly and to reduce populations in regions where this species is endemic. Factory scale rearing is essential for SIT; however, artificial larval diets are known to affect the microbiome of Qfly, which may then affect fly performance. In this study, high-throughput Illumina sequencing was used to assess the Qfly microbiome in colonies reared, for five generations from nature, on two common artificial diets (carrot and gel). At generation five (G5), the microbiome was assessed in larvae, pupae, adult males and adult females and standard fly quality control parameters were assessed together with additional performance measures of mating propensity and survival under nutritional stress. At the genus level, bacterial communities were significantly different between the colonies reared on the two larval diets. However, communities converged at Phyla to family taxonomic levels. Bacterial genera of Morganella, Citrobacter, Providencia, and Burkholderia were highly abundant in all developmental stages of Qfly reared on the gel diet, when compared to the carrot diet. Despite abundance of these genera, a greater percentage of egg hatching, heavier pupal weight and a higher percentage of fliers were found in the Qfly reared on the gel diet. Mating propensity and survival under nutritional stress was similar for adult Qfly that had been reared on the two larval diets. Overall, our findings demonstrate that the artificial larval diet strongly influences the microbiome and quality control measures of Qfly, with likely downstream effects on performance of flies released in SIT programs. |
| first_indexed | 2024-12-19T14:17:53Z |
| format | Article |
| id | doaj.art-32f8a3aa2cc34f43a3d1ae5d7914e8f0 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T14:17:53Z |
| publishDate | 2020-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-32f8a3aa2cc34f43a3d1ae5d7914e8f02022-12-21T20:17:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-09-011110.3389/fmicb.2020.576156576156Artificial Larval Diet Mediates the Microbiome of Queensland Fruit FlyRajib Majumder0Rajib Majumder1Brodie Sutcliffe2Saleh Mohammad Adnan3Saleh Mohammad Adnan4Bishwo Mainali5Bernard C. Dominiak6Phillip W. Taylor7Toni A. Chapman8Toni A. Chapman9Applied BioSciences, Macquarie University, North Ryde, NSW, AustraliaBiosecurity and Food Safety, NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute (EMAI), Menangle, NSW, AustraliaBiosecurity and Food Safety, NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute (EMAI), Menangle, NSW, AustraliaApplied BioSciences, Macquarie University, North Ryde, NSW, AustraliaDepartment of Entomology, Bangladesh Agricultural University, Mymensingh, BangladeshApplied BioSciences, Macquarie University, North Ryde, NSW, AustraliaBiosecurity and Food Safety, NSW Department of Primary Industries, Orange, NSW, AustraliaApplied BioSciences, Macquarie University, North Ryde, NSW, AustraliaApplied BioSciences, Macquarie University, North Ryde, NSW, AustraliaBiosecurity and Food Safety, NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute (EMAI), Menangle, NSW, AustraliaLarval diets used for artificial rearing can have a significant effect on insect biology. The Queensland fruit fly (aka “Qfly”), Bactrocera tryoni (Froggatt) (Diptera: Tephritidae), is one of the greatest challenges for fruit growers in Australia. The sterile insect technique (SIT) is being developed to manage outbreaks in regions that remain free of Qfly and to reduce populations in regions where this species is endemic. Factory scale rearing is essential for SIT; however, artificial larval diets are known to affect the microbiome of Qfly, which may then affect fly performance. In this study, high-throughput Illumina sequencing was used to assess the Qfly microbiome in colonies reared, for five generations from nature, on two common artificial diets (carrot and gel). At generation five (G5), the microbiome was assessed in larvae, pupae, adult males and adult females and standard fly quality control parameters were assessed together with additional performance measures of mating propensity and survival under nutritional stress. At the genus level, bacterial communities were significantly different between the colonies reared on the two larval diets. However, communities converged at Phyla to family taxonomic levels. Bacterial genera of Morganella, Citrobacter, Providencia, and Burkholderia were highly abundant in all developmental stages of Qfly reared on the gel diet, when compared to the carrot diet. Despite abundance of these genera, a greater percentage of egg hatching, heavier pupal weight and a higher percentage of fliers were found in the Qfly reared on the gel diet. Mating propensity and survival under nutritional stress was similar for adult Qfly that had been reared on the two larval diets. Overall, our findings demonstrate that the artificial larval diet strongly influences the microbiome and quality control measures of Qfly, with likely downstream effects on performance of flies released in SIT programs.https://www.frontiersin.org/article/10.3389/fmicb.2020.576156/fullTephritidaegut bacteriaIllumina sequencingdevelopmentdomesticationmating |
| spellingShingle | Rajib Majumder Rajib Majumder Brodie Sutcliffe Saleh Mohammad Adnan Saleh Mohammad Adnan Bishwo Mainali Bernard C. Dominiak Phillip W. Taylor Toni A. Chapman Toni A. Chapman Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly Frontiers in Microbiology Tephritidae gut bacteria Illumina sequencing development domestication mating |
| title | Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly |
| title_full | Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly |
| title_fullStr | Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly |
| title_full_unstemmed | Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly |
| title_short | Artificial Larval Diet Mediates the Microbiome of Queensland Fruit Fly |
| title_sort | artificial larval diet mediates the microbiome of queensland fruit fly |
| topic | Tephritidae gut bacteria Illumina sequencing development domestication mating |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.576156/full |
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