High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in Benin
Among the barriers to accessing adequate treatment and high-level monitoring for malaria febrile patients is the lack of effective prognostic markers. Neopterin, which is a marker of monocyte/macrophage activation, was found have increased during severe malaria. In this study, we used quantitative E...
| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/3/528 |
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| author | Samuel Odarkwei Blankson Lauriane Rietmeyer Patrick Tettey Liliane Dikroh Bernard Tornyigah Rafiou Adamou Azizath Moussiliou Caroline Padounou Annick Amoussou Benedicta Ayiedu Mensah Maroufou J. Alao Gordon Awandare Nicaise Tuikue Ndam Christian Roussilhon Rachida Tahar |
| author_facet | Samuel Odarkwei Blankson Lauriane Rietmeyer Patrick Tettey Liliane Dikroh Bernard Tornyigah Rafiou Adamou Azizath Moussiliou Caroline Padounou Annick Amoussou Benedicta Ayiedu Mensah Maroufou J. Alao Gordon Awandare Nicaise Tuikue Ndam Christian Roussilhon Rachida Tahar |
| author_sort | Samuel Odarkwei Blankson |
| collection | DOAJ |
| description | Among the barriers to accessing adequate treatment and high-level monitoring for malaria febrile patients is the lack of effective prognostic markers. Neopterin, which is a marker of monocyte/macrophage activation, was found have increased during severe malaria. In this study, we used quantitative ELISA in order to assess the levels of plasma soluble neopterin in 151 patients from a cohort of Beninese children with severe malaria. We evaluated the prognostic accuracy of this molecule in order to predict the outcome of the disease. Our results show that neopterin levels were not significantly different between patients with different forms of severe malaria, including severe non-cerebral malaria (SNCM) and cerebral malaria (CM). However, the levels of this molecule were found to be higher in patients with severe malarial anemia (SMA) among both CM and SNCM cases (<i>p</i>-value = 0.02). Additionally, the levels of this molecule were found to be higher in patients who died from these pathologies compared to those who survived among the two clinical groups (<i>p</i>-value < 0.0001) and within the same group (<i>p</i>-value < 0.0001 for the CM group, <i>p</i>-value = 0.0046 for the SNCM group). The AUC-ROC for fatality among all the severe cases was 0.77 with a 95%CI of (0.69–0.85). These results suggest that plasma neopterin levels constitute a potential biomarker for predicting fatality among severe falciparum malaria patients. |
| first_indexed | 2024-03-11T09:47:52Z |
| format | Article |
| id | doaj.art-32fd4faa6130449ea2033b9e9373847f |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T09:47:52Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-32fd4faa6130449ea2033b9e9373847f2023-11-16T16:26:05ZengMDPI AGDiagnostics2075-44182023-01-0113352810.3390/diagnostics13030528High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in BeninSamuel Odarkwei Blankson0Lauriane Rietmeyer1Patrick Tettey2Liliane Dikroh3Bernard Tornyigah4Rafiou Adamou5Azizath Moussiliou6Caroline Padounou7Annick Amoussou8Benedicta Ayiedu Mensah9Maroufou J. Alao10Gordon Awandare11Nicaise Tuikue Ndam12Christian Roussilhon13Rachida Tahar14MERIT, IRD, Université de Paris Cité, 75006 Paris, FranceMERIT, IRD, Université de Paris Cité, 75006 Paris, FranceDepartment of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra LG 54, GhanaDepartment of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra LG 54, GhanaMERIT, IRD, Université de Paris Cité, 75006 Paris, FranceInstitut de Recherche Clinique du Benin (IRCB), Calavi, BeninInstitut de Recherche Clinique du Benin (IRCB), Calavi, BeninCentre Hospitalier Universitaire de l’Oueme, Porto-Novo, BeninService de Pédiatrie, Centre Hospitalo-Universitaire, Suruléré (CHU-Suruléré), Cotonou, BeninDepartment of Immunology, Noguchi Memorial Institute for Medical Research, University of Ghana, Accra LG 54, GhanaDépartement de Pédiatrie, Hôpital Mère-Enfant la Lagune (CHUMEL), Cotonou, BeninWest African Centre for Cell Biology of Infectious Pathogens, Department of Biochemistry, Cell and Molecular Biology, College of Basic and Applied Sciences, University of Ghana, Accra LG 54, GhanaMERIT, IRD, Université de Paris Cité, 75006 Paris, FranceInstitut Pasteur, 28 Rue du Docteur Roux, 75015 Paris, FranceMERIT, IRD, Université de Paris Cité, 75006 Paris, FranceAmong the barriers to accessing adequate treatment and high-level monitoring for malaria febrile patients is the lack of effective prognostic markers. Neopterin, which is a marker of monocyte/macrophage activation, was found have increased during severe malaria. In this study, we used quantitative ELISA in order to assess the levels of plasma soluble neopterin in 151 patients from a cohort of Beninese children with severe malaria. We evaluated the prognostic accuracy of this molecule in order to predict the outcome of the disease. Our results show that neopterin levels were not significantly different between patients with different forms of severe malaria, including severe non-cerebral malaria (SNCM) and cerebral malaria (CM). However, the levels of this molecule were found to be higher in patients with severe malarial anemia (SMA) among both CM and SNCM cases (<i>p</i>-value = 0.02). Additionally, the levels of this molecule were found to be higher in patients who died from these pathologies compared to those who survived among the two clinical groups (<i>p</i>-value < 0.0001) and within the same group (<i>p</i>-value < 0.0001 for the CM group, <i>p</i>-value = 0.0046 for the SNCM group). The AUC-ROC for fatality among all the severe cases was 0.77 with a 95%CI of (0.69–0.85). These results suggest that plasma neopterin levels constitute a potential biomarker for predicting fatality among severe falciparum malaria patients.https://www.mdpi.com/2075-4418/13/3/528neopterinsevere malariacerebral malariaanemia<i>Plasmodium falciparum</i>Benin |
| spellingShingle | Samuel Odarkwei Blankson Lauriane Rietmeyer Patrick Tettey Liliane Dikroh Bernard Tornyigah Rafiou Adamou Azizath Moussiliou Caroline Padounou Annick Amoussou Benedicta Ayiedu Mensah Maroufou J. Alao Gordon Awandare Nicaise Tuikue Ndam Christian Roussilhon Rachida Tahar High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in Benin Diagnostics neopterin severe malaria cerebral malaria anemia <i>Plasmodium falciparum</i> Benin |
| title | High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in Benin |
| title_full | High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in Benin |
| title_fullStr | High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in Benin |
| title_full_unstemmed | High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in Benin |
| title_short | High Plasma Levels of Neopterin Are Associated with Increased Mortality among Children with Severe Malaria in Benin |
| title_sort | high plasma levels of neopterin are associated with increased mortality among children with severe malaria in benin |
| topic | neopterin severe malaria cerebral malaria anemia <i>Plasmodium falciparum</i> Benin |
| url | https://www.mdpi.com/2075-4418/13/3/528 |
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