Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming
This study investigated changes in meat and subcutaneous fat metabolomes and possible metabolic pathways related to prenatal nutrition in beef cattle. For this purpose, 18 Nellore bulls were used for meat sampling and 15 for fat sampling. The nutritional treatments during the gestation were: NP—not...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/14/1/9 |
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| author | Arícia Christofaro Fernandes Guilherme Henrique Gebim Polizel Roberta Cavalcante Cracco Fernando Augusto Correia Queiroz Cançado Geovana Camila Baldin Mirele Daiana Poleti José Bento Sterman Ferraz Miguel Henrique de Almeida Santana |
| author_facet | Arícia Christofaro Fernandes Guilherme Henrique Gebim Polizel Roberta Cavalcante Cracco Fernando Augusto Correia Queiroz Cançado Geovana Camila Baldin Mirele Daiana Poleti José Bento Sterman Ferraz Miguel Henrique de Almeida Santana |
| author_sort | Arícia Christofaro Fernandes |
| collection | DOAJ |
| description | This study investigated changes in meat and subcutaneous fat metabolomes and possible metabolic pathways related to prenatal nutrition in beef cattle. For this purpose, 18 Nellore bulls were used for meat sampling and 15 for fat sampling. The nutritional treatments during the gestation were: NP—not programmed or control, without protein-energy supplementation; PP—partially programmed, with protein-energy supplementation (0.3% of body weight (BW)) only in the final third of pregnancy; and FP—full programming, with protein-energy supplementation (0.3% of BW) during the entire pregnancy. The meat and fat samples were collected individually 24 h after slaughter, and the metabolites were extracted using a combination of chemical reagents and mechanical processes and subsequently quantified using liquid chromatography or flow injection coupled to mass spectrometry. The data obtained were submitted to principal component analysis (PCA), analysis of variance (ANOVA), and functional enrichment analysis, with a significance level of 5%. The PCA showed an overlap between the treatments for both meat and fat. In meat, 25 metabolites were statistically different between treatments (<i>p</i> ≤ 0.05), belonging to four classes (glycerophospholipids, amino acids, sphingolipids, and biogenic amine). In fat, 10 significant metabolites (<i>p</i> ≤ 0.05) were obtained in two classes (phosphatidylcholine and lysophosphatidylcholine). The functional enrichment analysis showed alterations in the aminoacyl-tRNA pathway in meat (<i>p</i> = 0.030); however, there was no pathway enriched for fat. Fetal programming influenced the meat and fat metabolomes and the aminoacyl-tRNA metabolic pathway, which is an important candidate for the biological process linked to meat quality and related to fetal programming in beef cattle. |
| first_indexed | 2024-03-08T09:50:42Z |
| format | Article |
| id | doaj.art-32fe0de4299c495d8b27d1c0ee220f22 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-08T09:50:42Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-32fe0de4299c495d8b27d1c0ee220f222024-01-29T14:04:33ZengMDPI AGMetabolites2218-19892023-12-01141910.3390/metabo14010009Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal ProgrammingArícia Christofaro Fernandes0Guilherme Henrique Gebim Polizel1Roberta Cavalcante Cracco2Fernando Augusto Correia Queiroz Cançado3Geovana Camila Baldin4Mirele Daiana Poleti5José Bento Sterman Ferraz6Miguel Henrique de Almeida Santana7Department of Animal Science, College of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Veterinary Medicine, Faculty of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilDepartment of Animal Science, College of Animal Science and Food Engineering, University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga 13635-900, SP, BrazilThis study investigated changes in meat and subcutaneous fat metabolomes and possible metabolic pathways related to prenatal nutrition in beef cattle. For this purpose, 18 Nellore bulls were used for meat sampling and 15 for fat sampling. The nutritional treatments during the gestation were: NP—not programmed or control, without protein-energy supplementation; PP—partially programmed, with protein-energy supplementation (0.3% of body weight (BW)) only in the final third of pregnancy; and FP—full programming, with protein-energy supplementation (0.3% of BW) during the entire pregnancy. The meat and fat samples were collected individually 24 h after slaughter, and the metabolites were extracted using a combination of chemical reagents and mechanical processes and subsequently quantified using liquid chromatography or flow injection coupled to mass spectrometry. The data obtained were submitted to principal component analysis (PCA), analysis of variance (ANOVA), and functional enrichment analysis, with a significance level of 5%. The PCA showed an overlap between the treatments for both meat and fat. In meat, 25 metabolites were statistically different between treatments (<i>p</i> ≤ 0.05), belonging to four classes (glycerophospholipids, amino acids, sphingolipids, and biogenic amine). In fat, 10 significant metabolites (<i>p</i> ≤ 0.05) were obtained in two classes (phosphatidylcholine and lysophosphatidylcholine). The functional enrichment analysis showed alterations in the aminoacyl-tRNA pathway in meat (<i>p</i> = 0.030); however, there was no pathway enriched for fat. Fetal programming influenced the meat and fat metabolomes and the aminoacyl-tRNA metabolic pathway, which is an important candidate for the biological process linked to meat quality and related to fetal programming in beef cattle.https://www.mdpi.com/2218-1989/14/1/9meat qualitymetabolitesmuscular and adipose developmentpregnancyprenatal supplementation |
| spellingShingle | Arícia Christofaro Fernandes Guilherme Henrique Gebim Polizel Roberta Cavalcante Cracco Fernando Augusto Correia Queiroz Cançado Geovana Camila Baldin Mirele Daiana Poleti José Bento Sterman Ferraz Miguel Henrique de Almeida Santana Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming Metabolites meat quality metabolites muscular and adipose development pregnancy prenatal supplementation |
| title | Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming |
| title_full | Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming |
| title_fullStr | Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming |
| title_full_unstemmed | Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming |
| title_short | Metabolomics Changes in Meat and Subcutaneous Fat of Male Cattle Submitted to Fetal Programming |
| title_sort | metabolomics changes in meat and subcutaneous fat of male cattle submitted to fetal programming |
| topic | meat quality metabolites muscular and adipose development pregnancy prenatal supplementation |
| url | https://www.mdpi.com/2218-1989/14/1/9 |
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