Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells
Lectins (carbohydrate-binding proteins) are able to distinguish different patterns of glycosylation on cell surfaces. This study investigated the effects of lectins from <i>Alpinia purpurata</i> inflorescence (ApuL) and <i>Schinus terebinthifolia</i> leaf (SteLL) on the viabi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Macromol |
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| Online Access: | https://www.mdpi.com/2673-6209/3/2/18 |
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| author | Jéssica de Santana Brito Amanda de Oliveira Marinho Leydianne Leite de Siqueira Patriota Wyndly Daniel Cardoso Gaião Diego José Lira Torres Patrícia Maria Guedes Paiva Virgínia Maria Barros de Lorena Cláudio Gabriel Rodrigues Márcia Bezerra da Silva Thiago Henrique Napoleão |
| author_facet | Jéssica de Santana Brito Amanda de Oliveira Marinho Leydianne Leite de Siqueira Patriota Wyndly Daniel Cardoso Gaião Diego José Lira Torres Patrícia Maria Guedes Paiva Virgínia Maria Barros de Lorena Cláudio Gabriel Rodrigues Márcia Bezerra da Silva Thiago Henrique Napoleão |
| author_sort | Jéssica de Santana Brito |
| collection | DOAJ |
| description | Lectins (carbohydrate-binding proteins) are able to distinguish different patterns of glycosylation on cell surfaces. This study investigated the effects of lectins from <i>Alpinia purpurata</i> inflorescence (ApuL) and <i>Schinus terebinthifolia</i> leaf (SteLL) on the viability of human leukemia cells (K562, chronic myeloid leukemia; JURKAT, acute lymphoblastic leukemia) and mesenchymal stem cells (MSCs) from human umbilical cords. In addition, possible immunomodulatory effects of ApuL and SteLL on MSCs were assessed by determining cytokine levels in cultures. ApuL reduced the viability of JURKAT cells (IC<sub>50</sub>: 12.5 μg/mL), inducing both apoptosis and necrosis. For K562 cells, ApuL at 50 µg/mL caused a decrease in viability, but of only 8.8%. Conversely, SteLL exerted a cytotoxic effect on K562 (IC<sub>50</sub>: 6.0 μg/mL), inducing apoptosis, while it was not cytotoxic to JURKAT. ApuL and SteLL (0.19–100 μg/mL) did not decrease MSCs viability. Treatment with ApuL strongly suppressed (99.5% reduction) the release of IL-6 by MSCs. SteLL also reduced the levels of this cytokine in culture supernatant. In conclusion, ApuL and SteLL showed potential to reduce the viability of leukemia cells, as well as immunomodulatory effect on MSCs without being toxic to them. These biological properties can be explored biomedically and biotechnologically in the future. |
| first_indexed | 2024-03-11T02:13:15Z |
| format | Article |
| id | doaj.art-3301370f386d4d69bc13f533a39ecaec |
| institution | Directory Open Access Journal |
| issn | 2673-6209 |
| language | English |
| last_indexed | 2024-03-11T02:13:15Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Macromol |
| spelling | doaj.art-3301370f386d4d69bc13f533a39ecaec2023-11-18T11:21:31ZengMDPI AGMacromol2673-62092023-05-013229030210.3390/macromol3020018Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem CellsJéssica de Santana Brito0Amanda de Oliveira Marinho1Leydianne Leite de Siqueira Patriota2Wyndly Daniel Cardoso Gaião3Diego José Lira Torres4Patrícia Maria Guedes Paiva5Virgínia Maria Barros de Lorena6Cláudio Gabriel Rodrigues7Márcia Bezerra da Silva8Thiago Henrique Napoleão9Departamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Imunologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50740-465, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Imunologia, Instituto Aggeu Magalhães, Fundação Oswaldo Cruz, Recife 50740-465, Pernambuco, BrazilDepartamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Biofísica e Radiobiologia, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilDepartamento de Bioquímica, Centro de Biociências, Universidade Federal de Pernambuco, Recife 50670-901, Pernambuco, BrazilLectins (carbohydrate-binding proteins) are able to distinguish different patterns of glycosylation on cell surfaces. This study investigated the effects of lectins from <i>Alpinia purpurata</i> inflorescence (ApuL) and <i>Schinus terebinthifolia</i> leaf (SteLL) on the viability of human leukemia cells (K562, chronic myeloid leukemia; JURKAT, acute lymphoblastic leukemia) and mesenchymal stem cells (MSCs) from human umbilical cords. In addition, possible immunomodulatory effects of ApuL and SteLL on MSCs were assessed by determining cytokine levels in cultures. ApuL reduced the viability of JURKAT cells (IC<sub>50</sub>: 12.5 μg/mL), inducing both apoptosis and necrosis. For K562 cells, ApuL at 50 µg/mL caused a decrease in viability, but of only 8.8%. Conversely, SteLL exerted a cytotoxic effect on K562 (IC<sub>50</sub>: 6.0 μg/mL), inducing apoptosis, while it was not cytotoxic to JURKAT. ApuL and SteLL (0.19–100 μg/mL) did not decrease MSCs viability. Treatment with ApuL strongly suppressed (99.5% reduction) the release of IL-6 by MSCs. SteLL also reduced the levels of this cytokine in culture supernatant. In conclusion, ApuL and SteLL showed potential to reduce the viability of leukemia cells, as well as immunomodulatory effect on MSCs without being toxic to them. These biological properties can be explored biomedically and biotechnologically in the future.https://www.mdpi.com/2673-6209/3/2/18anticancerstem cellscarbohydrate-binding proteinsBrazilian pepper treered ginger |
| spellingShingle | Jéssica de Santana Brito Amanda de Oliveira Marinho Leydianne Leite de Siqueira Patriota Wyndly Daniel Cardoso Gaião Diego José Lira Torres Patrícia Maria Guedes Paiva Virgínia Maria Barros de Lorena Cláudio Gabriel Rodrigues Márcia Bezerra da Silva Thiago Henrique Napoleão Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells Macromol anticancer stem cells carbohydrate-binding proteins Brazilian pepper tree red ginger |
| title | Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells |
| title_full | Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells |
| title_fullStr | Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells |
| title_full_unstemmed | Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells |
| title_short | Effects of Lectins from <i>Alpinia purpurata</i> Inflorescence (ApuL) and <i>Schinus terebinthifolia</i> Leaf (SteLL) on Human Leukemic Cell Lines and Mesenchymal Stem Cells |
| title_sort | effects of lectins from i alpinia purpurata i inflorescence apul and i schinus terebinthifolia i leaf stell on human leukemic cell lines and mesenchymal stem cells |
| topic | anticancer stem cells carbohydrate-binding proteins Brazilian pepper tree red ginger |
| url | https://www.mdpi.com/2673-6209/3/2/18 |
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