Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues
Background: Leucine zipper protein 2 (LUZP2) is a vital gene encoding leucine zipper protein. It is of great importance in the incidence and progress of several human cancers. However, little is known about the role and clinical effects of LUZP2 in prostate cancer (PCa). Therefore, it is crucial to...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-09-01
|
| Series: | Electronic Journal of Biotechnology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345822000239 |
| _version_ | 1811210367371575296 |
|---|---|
| author | Sheng-Hua Li Yuan-Ping Yang Rong-Quan He Juan He Xiao Feng Xiao-Xiang Yu Yu-Xuan Yao Guan-Lan Zhang Jie Li Ji-Wen Cheng Gang Chen Zhi-Guang Huang |
| author_facet | Sheng-Hua Li Yuan-Ping Yang Rong-Quan He Juan He Xiao Feng Xiao-Xiang Yu Yu-Xuan Yao Guan-Lan Zhang Jie Li Ji-Wen Cheng Gang Chen Zhi-Guang Huang |
| author_sort | Sheng-Hua Li |
| collection | DOAJ |
| description | Background: Leucine zipper protein 2 (LUZP2) is a vital gene encoding leucine zipper protein. It is of great importance in the incidence and progress of several human cancers. However, little is known about the role and clinical effects of LUZP2 in prostate cancer (PCa). Therefore, it is crucial to unravel the clinicopathological value of LUZP2 in PCa. In all, 1467 PCa and 549 non-prostate cancer (non-PCa) prostate samples were collected from mRNA chip and RNA-sequencing datasets. The protein levels of LUZP2 were verified in 91 prostate gland tissues by in-house immunohistochemistry (IHC). The standardized mean difference (SMD) was calculated to analyze LUZP2 expression. Survival analysis was also conducted to explore the prognostic significance of LUZP2 in PCa. R software was employed to identify the upregulated differently expressed genes (up-DEGs) and coexpressed genes (CEGs) of LUZP2. Additionally, we explored the prospective molecular mechanism of CEGs of LUZP2 through GO and KEGG pathway analyses. Results: Compared with non-PCa, LUZP2 showed predominantly higher expression in PCa (SMD = 1.05, AUC = 0.88). IHC indicated the protein expression level of LUZP2 was consistently upregulated in PCa tissues (SMD = 2.23, 95%CI: 1.67–2.79). LUZP2 upregulation had an AUC of 0.88 (95%CI: 0.85–0.90) to distinguish PCa from non-PCa tissues. KEGG pathway analysis showed that the pathways of amino sugar and nucleoside sugar metabolism were chiefly enriched with the LUZP2 CEGs in PCa. Conclusion:: LUZP2 upregulation might play a promoting function in the occurrence of PCa.How to cite: Li S-H, Yang Y-P, He R-Q, et al. Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.06.001 |
| first_indexed | 2024-04-12T04:54:25Z |
| format | Article |
| id | doaj.art-330290dee27a40ebba176a238d738e37 |
| institution | Directory Open Access Journal |
| issn | 0717-3458 |
| language | English |
| last_indexed | 2024-04-12T04:54:25Z |
| publishDate | 2022-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj.art-330290dee27a40ebba176a238d738e372022-12-22T03:47:11ZengElsevierElectronic Journal of Biotechnology0717-34582022-09-0159112Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissuesSheng-Hua Li0Yuan-Ping Yang1Rong-Quan He2Juan He3Xiao Feng4Xiao-Xiang Yu5Yu-Xuan Yao6Guan-Lan Zhang7Jie Li8Ji-Wen Cheng9Gang Chen10Zhi-Guang Huang11Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Medical Oncology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Radiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Urology, The 923rd Hospital of Chinese People's Liberation Army Joint Logistics Support Force, Nanning, PR ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR ChinaDepartment of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, PR China; Corresponding author.Background: Leucine zipper protein 2 (LUZP2) is a vital gene encoding leucine zipper protein. It is of great importance in the incidence and progress of several human cancers. However, little is known about the role and clinical effects of LUZP2 in prostate cancer (PCa). Therefore, it is crucial to unravel the clinicopathological value of LUZP2 in PCa. In all, 1467 PCa and 549 non-prostate cancer (non-PCa) prostate samples were collected from mRNA chip and RNA-sequencing datasets. The protein levels of LUZP2 were verified in 91 prostate gland tissues by in-house immunohistochemistry (IHC). The standardized mean difference (SMD) was calculated to analyze LUZP2 expression. Survival analysis was also conducted to explore the prognostic significance of LUZP2 in PCa. R software was employed to identify the upregulated differently expressed genes (up-DEGs) and coexpressed genes (CEGs) of LUZP2. Additionally, we explored the prospective molecular mechanism of CEGs of LUZP2 through GO and KEGG pathway analyses. Results: Compared with non-PCa, LUZP2 showed predominantly higher expression in PCa (SMD = 1.05, AUC = 0.88). IHC indicated the protein expression level of LUZP2 was consistently upregulated in PCa tissues (SMD = 2.23, 95%CI: 1.67–2.79). LUZP2 upregulation had an AUC of 0.88 (95%CI: 0.85–0.90) to distinguish PCa from non-PCa tissues. KEGG pathway analysis showed that the pathways of amino sugar and nucleoside sugar metabolism were chiefly enriched with the LUZP2 CEGs in PCa. Conclusion:: LUZP2 upregulation might play a promoting function in the occurrence of PCa.How to cite: Li S-H, Yang Y-P, He R-Q, et al. Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues. Electron J Biotechnol 2022;59. https://doi.org/10.1016/j.ejbt.2022.06.001http://www.sciencedirect.com/science/article/pii/S0717345822000239CancerData integrationFunctional enrichment analysisGene chipLeucine zipper protein 2Mutation analysis |
| spellingShingle | Sheng-Hua Li Yuan-Ping Yang Rong-Quan He Juan He Xiao Feng Xiao-Xiang Yu Yu-Xuan Yao Guan-Lan Zhang Jie Li Ji-Wen Cheng Gang Chen Zhi-Guang Huang Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues Electronic Journal of Biotechnology Cancer Data integration Functional enrichment analysis Gene chip Leucine zipper protein 2 Mutation analysis |
| title | Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues |
| title_full | Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues |
| title_fullStr | Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues |
| title_full_unstemmed | Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues |
| title_short | Comprehensive expression analysis reveals upregulated LUZP2 in prostate cancer tissues |
| title_sort | comprehensive expression analysis reveals upregulated luzp2 in prostate cancer tissues |
| topic | Cancer Data integration Functional enrichment analysis Gene chip Leucine zipper protein 2 Mutation analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0717345822000239 |
| work_keys_str_mv | AT shenghuali comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT yuanpingyang comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT rongquanhe comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT juanhe comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT xiaofeng comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT xiaoxiangyu comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT yuxuanyao comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT guanlanzhang comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT jieli comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT jiwencheng comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT gangchen comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues AT zhiguanghuang comprehensiveexpressionanalysisrevealsupregulatedluzp2inprostatecancertissues |