Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>)
Rainbow trout with gene editing-induced reductions in serum insulin-like growth factor binding protein (IGFBP)-2b exhibit similar growth performance compared to fish without IGFBP-2b gene disruption. The objective of this study is to determine how the components of the insulin-like growth factor (IG...
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| Format: | Article |
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MDPI AG
2020-12-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/12/1488 |
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| author | Beth M. Cleveland Shiori Habara Jin Oikawa Lisa M. Radler Munetaka Shimizu |
| author_facet | Beth M. Cleveland Shiori Habara Jin Oikawa Lisa M. Radler Munetaka Shimizu |
| author_sort | Beth M. Cleveland |
| collection | DOAJ |
| description | Rainbow trout with gene editing-induced reductions in serum insulin-like growth factor binding protein (IGFBP)-2b exhibit similar growth performance compared to fish without IGFBP-2b gene disruption. The objective of this study is to determine how the components of the insulin-like growth factor (IGF)/IGFBP system respond to a reduction in serum IGFBP-2b abundance. Editing the IGFBP-2b genes in rainbow trout resulted in an 83% decrease in serum IGFBP-2b in mutants. This resulted in a 35% reduction in serum IGF-I, which was offset by reduced expression of hepatic <i>igfbp-1a2</i> and increased muscle <i>igfr-1a</i>; these responses suggest that an increased IGF-I signaling capacity offset reductions in serum IGF-I. During feed deprivation, the differential expression of <i>igfbp</i> genes supports the attenuation of the growth inhibitory response, likely due to the further reduction in serum IGF-I that alleviated the need for an IGF-inhibitory response. Unique <i>igfbp</i> expression patterns occurred during refeeding, suggesting an enhanced IGF-I signaling capacity in controls. Collectively, these findings support that the role of IGFBP-2b is to regulate serum IGF-I concentrations. The compensatory regulation of IGF/IGFBP system genes indicates that adjustments in other IGFBP, both circulating and at the local level, maintain IGF-I signaling at a level appropriate for the nutritional state of the fish. |
| first_indexed | 2024-03-10T14:09:33Z |
| format | Article |
| id | doaj.art-33052e0e28af4cbabd2ba140aca5d983 |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T14:09:33Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-33052e0e28af4cbabd2ba140aca5d9832023-11-21T00:16:34ZengMDPI AGGenes2073-44252020-12-011112148810.3390/genes11121488Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>)Beth M. Cleveland0Shiori Habara1Jin Oikawa2Lisa M. Radler3Munetaka Shimizu4National Center for Cool and Cold Water Aquaculture, United States Department of Agriculture/Agricultural Research Service, Leetown, WV 25430, USAGraduate School of Environmental Science, Hokkaido University, Sapporo 060-0808, JapanGraduate School of Environmental Science, Hokkaido University, Sapporo 060-0808, JapanNational Center for Cool and Cold Water Aquaculture, United States Department of Agriculture/Agricultural Research Service, Leetown, WV 25430, USAFaculty of Fisheries Sciences, Hokkaido University, Hakodate 041-8611, JapanRainbow trout with gene editing-induced reductions in serum insulin-like growth factor binding protein (IGFBP)-2b exhibit similar growth performance compared to fish without IGFBP-2b gene disruption. The objective of this study is to determine how the components of the insulin-like growth factor (IGF)/IGFBP system respond to a reduction in serum IGFBP-2b abundance. Editing the IGFBP-2b genes in rainbow trout resulted in an 83% decrease in serum IGFBP-2b in mutants. This resulted in a 35% reduction in serum IGF-I, which was offset by reduced expression of hepatic <i>igfbp-1a2</i> and increased muscle <i>igfr-1a</i>; these responses suggest that an increased IGF-I signaling capacity offset reductions in serum IGF-I. During feed deprivation, the differential expression of <i>igfbp</i> genes supports the attenuation of the growth inhibitory response, likely due to the further reduction in serum IGF-I that alleviated the need for an IGF-inhibitory response. Unique <i>igfbp</i> expression patterns occurred during refeeding, suggesting an enhanced IGF-I signaling capacity in controls. Collectively, these findings support that the role of IGFBP-2b is to regulate serum IGF-I concentrations. The compensatory regulation of IGF/IGFBP system genes indicates that adjustments in other IGFBP, both circulating and at the local level, maintain IGF-I signaling at a level appropriate for the nutritional state of the fish.https://www.mdpi.com/2073-4425/11/12/1488gene editingCRISPR/Cas9IGFIGFBPrainbow troutfeed deprivation |
| spellingShingle | Beth M. Cleveland Shiori Habara Jin Oikawa Lisa M. Radler Munetaka Shimizu Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>) Genes gene editing CRISPR/Cas9 IGF IGFBP rainbow trout feed deprivation |
| title | Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full | Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_fullStr | Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_full_unstemmed | Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_short | Compensatory Response of the Somatotropic Axis from IGFBP-2b Gene Editing in Rainbow Trout (<i>Oncorhynchus mykiss</i>) |
| title_sort | compensatory response of the somatotropic axis from igfbp 2b gene editing in rainbow trout i oncorhynchus mykiss i |
| topic | gene editing CRISPR/Cas9 IGF IGFBP rainbow trout feed deprivation |
| url | https://www.mdpi.com/2073-4425/11/12/1488 |
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