Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation
Brown rot fungi degrade wood in a two-step process in which enzymatic hydrolysis is preceded by an oxidative degradation phase. While a detailed understanding of the molecular processes during brown rot decay is mandatory for being able to better protect wooden products from this type of degradation...
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Frontiers Media S.A.
2021-09-01
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| Series: | Frontiers in Fungal Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/ffunb.2021.701579/full |
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| author | Martina Kölle Maria Augusta Crivelente Horta J. Philipp Benz J. Philipp Benz Annica Pilgård Annica Pilgård |
| author_facet | Martina Kölle Maria Augusta Crivelente Horta J. Philipp Benz J. Philipp Benz Annica Pilgård Annica Pilgård |
| author_sort | Martina Kölle |
| collection | DOAJ |
| description | Brown rot fungi degrade wood in a two-step process in which enzymatic hydrolysis is preceded by an oxidative degradation phase. While a detailed understanding of the molecular processes during brown rot decay is mandatory for being able to better protect wooden products from this type of degradation, the underlying mechanisms are still not fully understood. This is particularly true for wood that has been treated to increase its resistance against rot. In the present study, the two degradation phases were separated to study the impact of wood acetylation on the behavior of three brown rot fungi commonly used in wood durability testing. Transcriptomic data from two strains of Rhodonia placenta (FPRL280 and MAD-698) and Gloeophyllum trabeum were recorded to elucidate differences between the respective decay strategies. Clear differences were found between the two decay stages in all fungi. Moreover, strategies varied not only between species but also between the two strains of the same species. The responses to wood acetylation showed that decay is generally delayed and that parts of the process are attenuated. By hierarchical clustering, we could localize several transcription factors within gene clusters that were heavily affected by acetylation, especially in G. trabeum. The results suggest that regulatory circuits evolve rapidly and are probably the major cause behind the different decay strategies as observed even between the two strains of R. placenta. Identifying key genes in these processes can help in decay detection and identification of the fungi by biomarker selection, and also be informative for other fields, such as fiber modification by biocatalysts and the generation of biochemical platform chemicals for biorefinery applications. |
| first_indexed | 2024-12-22T12:41:29Z |
| format | Article |
| id | doaj.art-330e1aed140a420ea49f37e672df51a9 |
| institution | Directory Open Access Journal |
| issn | 2673-6128 |
| language | English |
| last_indexed | 2024-12-22T12:41:29Z |
| publishDate | 2021-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Fungal Biology |
| spelling | doaj.art-330e1aed140a420ea49f37e672df51a92022-12-21T18:25:25ZengFrontiers Media S.A.Frontiers in Fungal Biology2673-61282021-09-01210.3389/ffunb.2021.701579701579Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood AcetylationMartina Kölle0Maria Augusta Crivelente Horta1J. Philipp Benz2J. Philipp Benz3Annica Pilgård4Annica Pilgård5Chair of Wood Science, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, GermanyProfessorship of Fungal Biotechnology in Wood Science, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, GermanyProfessorship of Fungal Biotechnology in Wood Science, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, GermanyInstitute of Advanced Study, Technical University of Munich, Munich, GermanyChair of Wood Science, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Munich, GermanyBiobased Materials, Bioeconomy, RISE Research Institutes of Sweden, Borås, SwedenBrown rot fungi degrade wood in a two-step process in which enzymatic hydrolysis is preceded by an oxidative degradation phase. While a detailed understanding of the molecular processes during brown rot decay is mandatory for being able to better protect wooden products from this type of degradation, the underlying mechanisms are still not fully understood. This is particularly true for wood that has been treated to increase its resistance against rot. In the present study, the two degradation phases were separated to study the impact of wood acetylation on the behavior of three brown rot fungi commonly used in wood durability testing. Transcriptomic data from two strains of Rhodonia placenta (FPRL280 and MAD-698) and Gloeophyllum trabeum were recorded to elucidate differences between the respective decay strategies. Clear differences were found between the two decay stages in all fungi. Moreover, strategies varied not only between species but also between the two strains of the same species. The responses to wood acetylation showed that decay is generally delayed and that parts of the process are attenuated. By hierarchical clustering, we could localize several transcription factors within gene clusters that were heavily affected by acetylation, especially in G. trabeum. The results suggest that regulatory circuits evolve rapidly and are probably the major cause behind the different decay strategies as observed even between the two strains of R. placenta. Identifying key genes in these processes can help in decay detection and identification of the fungi by biomarker selection, and also be informative for other fields, such as fiber modification by biocatalysts and the generation of biochemical platform chemicals for biorefinery applications.https://www.frontiersin.org/articles/10.3389/ffunb.2021.701579/fullRhodonia placentaPostia placentaGloeophyllum trabeumbrown rot decaytranscriptome comparisonwood degradation and deterioration |
| spellingShingle | Martina Kölle Maria Augusta Crivelente Horta J. Philipp Benz J. Philipp Benz Annica Pilgård Annica Pilgård Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation Frontiers in Fungal Biology Rhodonia placenta Postia placenta Gloeophyllum trabeum brown rot decay transcriptome comparison wood degradation and deterioration |
| title | Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation |
| title_full | Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation |
| title_fullStr | Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation |
| title_full_unstemmed | Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation |
| title_short | Comparative Transcriptomics During Brown Rot Decay in Three Fungi Reveals Strain-Specific Degradative Strategies and Responses to Wood Acetylation |
| title_sort | comparative transcriptomics during brown rot decay in three fungi reveals strain specific degradative strategies and responses to wood acetylation |
| topic | Rhodonia placenta Postia placenta Gloeophyllum trabeum brown rot decay transcriptome comparison wood degradation and deterioration |
| url | https://www.frontiersin.org/articles/10.3389/ffunb.2021.701579/full |
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