Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)
Abstract Background In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice (Oryza sativa). To...
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BMC
2020-03-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://link.springer.com/article/10.1186/s12870-020-2298-5 |
| _version_ | 1818821832911880192 |
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| author | Eui-Jung Kim Sung-Wook Park Woo-Jong Hong Jeniffer Silva Wanqi Liang Dabing Zhang Ki-Hong Jung Yu-Jin Kim |
| author_facet | Eui-Jung Kim Sung-Wook Park Woo-Jong Hong Jeniffer Silva Wanqi Liang Dabing Zhang Ki-Hong Jung Yu-Jin Kim |
| author_sort | Eui-Jung Kim |
| collection | DOAJ |
| description | Abstract Background In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice (Oryza sativa). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice. Results Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis thaliana. In subcellular localization analysis of the four RopGEFs through tobacco (Nicotiana benthamiana) infiltration, four proteins were predominantly identified in plasma membrane. Moreover, double mutants of RopGEF2/8 exhibited reduced pollen germination, causing partial male sterility. These genes possess unique cis-acting elements in their promoters compared with the other RopGEF genes. Conclusions In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the expression pattern, promoter analysis, and evolutionary relationship of the RopGEF family were studied compared with Arabidopsis. Our study indicated that four RopGEF genes might function during pollen germination in distinct subcellular localization. Our study could provide valuable information on the functional study of RopGEF in rice. |
| first_indexed | 2024-12-18T23:14:28Z |
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| id | doaj.art-330e245e49ce4159b7db2538b6f9f9f0 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-12-18T23:14:28Z |
| publishDate | 2020-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-330e245e49ce4159b7db2538b6f9f9f02022-12-21T20:48:16ZengBMCBMC Plant Biology1471-22292020-03-0120111510.1186/s12870-020-2298-5Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa)Eui-Jung Kim0Sung-Wook Park1Woo-Jong Hong2Jeniffer Silva3Wanqi Liang4Dabing Zhang5Ki-Hong Jung6Yu-Jin Kim7Graduate School of Biotechnology and Crop Biotech Institute, Kyung Hee UniversityGraduate School of Biotechnology and Crop Biotech Institute, Kyung Hee UniversityGraduate School of Biotechnology and Crop Biotech Institute, Kyung Hee UniversityGraduate School of Biotechnology and Crop Biotech Institute, Kyung Hee UniversityJoint International Research Laboratory of Metabolic and Developmental Sciences, State Key Laboratory of Hybrid Rice, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University–University of Adelaide Joint Centre for Agriculture and Health, Shanghai Jiao Tong UniversityGraduate School of Biotechnology and Crop Biotech Institute, Kyung Hee UniversityGraduate School of Biotechnology and Crop Biotech Institute, Kyung Hee UniversityGraduate School of Biotechnology and Crop Biotech Institute, Kyung Hee UniversityAbstract Background In plants, the key roles played by RopGEF-mediated ROP signaling in diverse processes, including polar tip growth, have been identified. Despite their important roles in reproduction, a comprehensive analysis of RopGEF members has not yet been performed in rice (Oryza sativa). To determine whether RopGEF regulators are involved in rice pollen tube growth, we performed genome-wide analysis of this family in rice. Results Phylogenomic and meta-expression analysis of eleven RopGEFs in rice showed that four genes were preferentially expressed in mature pollen. These four genes contain the plant-specific Rop nucleotide exchanger (PRONE) domain and possible phosphorylated residues, suggesting a conserved role in polar tip growth with Arabidopsis thaliana. In subcellular localization analysis of the four RopGEFs through tobacco (Nicotiana benthamiana) infiltration, four proteins were predominantly identified in plasma membrane. Moreover, double mutants of RopGEF2/8 exhibited reduced pollen germination, causing partial male sterility. These genes possess unique cis-acting elements in their promoters compared with the other RopGEF genes. Conclusions In this study, four RopGEF genes were identified as pollen-specific gene in eleven members of rice, and the expression pattern, promoter analysis, and evolutionary relationship of the RopGEF family were studied compared with Arabidopsis. Our study indicated that four RopGEF genes might function during pollen germination in distinct subcellular localization. Our study could provide valuable information on the functional study of RopGEF in rice.http://link.springer.com/article/10.1186/s12870-020-2298-5RopGEFROP/RacPollenOryza sativaGene family |
| spellingShingle | Eui-Jung Kim Sung-Wook Park Woo-Jong Hong Jeniffer Silva Wanqi Liang Dabing Zhang Ki-Hong Jung Yu-Jin Kim Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa) BMC Plant Biology RopGEF ROP/Rac Pollen Oryza sativa Gene family |
| title | Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa) |
| title_full | Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa) |
| title_fullStr | Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa) |
| title_full_unstemmed | Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa) |
| title_short | Genome-wide analysis of RopGEF gene family to identify genes contributing to pollen tube growth in rice (Oryza sativa) |
| title_sort | genome wide analysis of ropgef gene family to identify genes contributing to pollen tube growth in rice oryza sativa |
| topic | RopGEF ROP/Rac Pollen Oryza sativa Gene family |
| url | http://link.springer.com/article/10.1186/s12870-020-2298-5 |
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